Imaging ellipsometry combined with surface plasmon resonance for real-time biospecific interaction analysis

2005 ◽  
Author(s):  
Won Chegal ◽  
Hyun Mo Cho ◽  
Yong Jai Cho ◽  
Young Pil Kim ◽  
Hak Sung Kim
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Interaction Analysis ◽  
Imaging Ellipsometry ◽  
Biospecific Interaction

scholarly journals Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance

2012 ◽  
Vol 2012 ◽  
pp. 1-19 ◽  
Author(s):  
Markus Ritzefeld ◽  
Norbert Sewald
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Dna Sequences ◽  
Plasmon Resonance ◽  
Interaction Analysis ◽  
Physical Phenomenon ◽  
Dna Interactions ◽  
Protein Dna Interactions ◽  
Rna Interaction

Several proteins, like transcription factors, bind to certain DNA sequences, thereby regulating biochemical pathways that determine the fate of the corresponding cell. Due to these key positions, it is indispensable to analyze protein-DNA interactions and to identify their mode of action. Surface plasmon resonance is a label-free method that facilitates the elucidation of real-time kinetics of biomolecular interactions. In this article, we focus on this biosensor-based method and provide a detailed guide how SPR can be utilized to study binding of proteins to oligonucleotides. After a description of the physical phenomenon and the instrumental realization including fiber-optic-based SPR and SPR imaging, we will continue with a survey of immobilization methods. Subsequently, we will focus on the optimization of the experiment, expose pitfalls, and introduce how data should be analyzed and published. Finally, we summarize several interesting publications of the last decades dealing with protein-DNA and RNA interaction analysis by SPR.

scholarly journals Surface Plasmon Resonance for Detection of Genetically Modified Organisms in the Food Supply

2006 ◽  
Vol 89 (3) ◽  
pp. 893-897 ◽  
Author(s):  
Roberto Gambari ◽  
Giordana Feriotto
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Genetically Modified Organisms ◽  
Interaction Analysis ◽  
Genetically Modified ◽  
Research Field ◽  
Pcr Products ◽  
Value Determination ◽  
Biospecific Interaction

Abstract A review is presented demonstrating that biospecific interaction analysis, using surface plasmon resonance (SPR) and biosensor technologies is a simple, rapid, and automatable approach to detect genetically modified organisms (GMOs). Using SPR, we were able to monitor in real-time the hybridization between oligonucleotide or polymerase chain reaction (PCR)-generated probes and target single-stranded PCR products obtained by using as substrates DNA isolated from normal or transgenic soybean and maize. This procedure allows a one-step, nonradioactive detection of GMOs. PCR-generated probes are far more efficient in detecting GMOs than are oligodeoxyribonucleotide probes. This is expected to be a very important parameter, because information on low percentage of GMOs is of great value. Determination of the ability of SPR-based analysis to quantify GMOs should be considered a major research field for future studies, especially for the analyses of food supplies.

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