Exploring the potential of Raman and resonance Raman spectroscopy for quantitative analysis of duplex DNA

2005 ◽  
Author(s):  
H. G. Schulze ◽  
A. Bass ◽  
C. Addison ◽  
C. Hughesman ◽  
A. P. So ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Quantitative Analysis ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Duplex Dna

Quantitative Detection of Alcian Blue 8GX in the Low Concentration Range Using Surface-Enhanced Resonance Raman Spectroscopy

2000 ◽  
Vol 54 (3) ◽  
pp. 384-389 ◽  
Author(s):  
Iqbal T. Shadi ◽  
Babur Z. Chowdhry ◽  
Martin J. Snowden ◽  
Robert Withnall
Keyword(s):  
Raman Spectroscopy ◽  
Quantitative Analysis ◽  
Alcian Blue ◽  
Linear Correlation ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Good Linear ◽  
Surface Enhanced ◽  
Signal Intensities ◽  
Good Linear Correlation

The viability of the application of surface-enhanced resonance Raman spectroscopy (SERRS) to the quantitative analysis of the dye alcian blue 8GX has been examined with the use of citrate- and borohydride-reduced silver colloids. A good linear correlation is observed for the dependence of the SERRS signal intensities at 1344 and 1536 cm−1 ( R = 0.992 and 0.994, respectively) on dye concentrations in the range of 10−9–10−7 M, when using the citrate-reduced silver sol. Likewise, a good linear correlation is also observed for the dependence of the SERRS signal intensities at 1344 and 1536 cm−1 ( R = 0.978 and 0.959, respectively) on dye concentrations in the range 10−10–10−9 M, when using the borohydride-reduced silver sol. At concentrations of dye above 10−7 M, the concentration dependence of the SERRS signals is nonlinear, almost certainly due to the coverage of the surface of the colloidal silver particles by the dye being in excess of a full monolayer. However, at concentrations of alcian blue greater than 10−7 M, resonance Raman spectroscopy (RRS) can be employed for quantitative analysis of the dye. When performing RRS, using methanol as an internal standard, we observed a good linear correlation for the dependence of the signal intensities at 1344 and 1536 cm−1 ( R = 0.995 for both bands) on the dye concentration in the 10−6–10−3 M range. The limits of detection of alcian blue by SERRS using a citrate-reduced silver sol, SERRS using a borohydride-reduced silver sol, RRS, and visible absorption spectroscopy are found to be 26 ppb, 203 ppt, 2 ppm, and 0.7 ppm, respectively.

scholarly journals Structure of the retinal chromophore in sensory rhodopsin I from resonance Raman spectroscopy

1989 ◽  
Vol 264 (31) ◽  
pp. 18280-18283
Author(s):  
S P Fodor ◽  
R Gebhard ◽  
J Lugtenburg ◽  
R A Bogomolni ◽  
R A Mathies
Keyword(s):  
Raman Spectroscopy ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Sensory Rhodopsin ◽  
Retinal Chromophore ◽  
Sensory Rhodopsin I
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