Three-dimensional superresolution of focus-region of high numerical aperture objective lens: applied for multi-photon-absorption irradiation

2005 ◽  
Author(s):  
Ngoc Diep Lai ◽  
Jian Hung Lin ◽  
Po Wen Chen ◽  
Jaw Luen Tang ◽  
Chia Chen Hsu
Keyword(s):  
Three Dimensional ◽  
Numerical Aperture ◽  
Photon Absorption ◽  
Objective Lens ◽  
High Numerical Aperture

Controlling aspect ratio of focal spots of high numerical aperture objective lens in multi-photon absorption process

2006 ◽  
Vol 258 (2) ◽  
pp. 97-102 ◽  
Author(s):  
Ngoc Diep Lai ◽  
Jian Hung Lin ◽  
Po Wen Chen ◽  
Jaw Luen Tang ◽  
Chia Chen Hsu
Keyword(s):  
Aspect Ratio ◽  
Numerical Aperture ◽  
Photon Absorption ◽  
Objective Lens ◽  
Absorption Process ◽  
High Numerical Aperture

scholarly journals Focal Surface Detection of High Numerical Aperture Objective Lens Based on Differential Astigmatic Method

2021 ◽  
Vol 13 (4) ◽  
pp. 1-8
Author(s):  
Jia-Lin Du ◽  
Wei Yan ◽  
Li-Wei Liu ◽  
Fan-Xing Li ◽  
Fu-Ping Peng ◽  
...  
Keyword(s):  
Numerical Aperture ◽  
Objective Lens ◽  
High Numerical Aperture ◽  
Surface Detection ◽  
Focal Surface

scholarly journals Dynamic three-dimensional multifocal spots in high numerical-aperture objectives

2017 ◽  
Vol 25 (20) ◽  
pp. 24756 ◽  
Author(s):  
Linwei Zhu ◽  
Rui Yang ◽  
Dawei Zhang ◽  
Junjie Yu ◽  
Jiannong Chen
Keyword(s):  
Three Dimensional ◽  
Numerical Aperture ◽  
High Numerical Aperture

Calibration of polarization effect of a high-numerical-aperture objective lens with Mueller matrix polarimetry

2018 ◽  
Vol 30 (2) ◽  
pp. 025201
Author(s):  
Chao Chen ◽  
Xiuguo Chen ◽  
Honggang Gu ◽  
Hao Jiang ◽  
Chuanwei Zhang ◽  
...  
Keyword(s):  
Polarization Effect ◽  
Numerical Aperture ◽  
Mueller Matrix ◽  
Objective Lens ◽  
High Numerical Aperture

Evaluation of Anisotropic Biaxial Stress by Raman Spectroscopy with a High Numerical Aperture Immersion Objective Lens

2010 ◽  
Vol 49 (4) ◽  
pp. 04DA21 ◽  
Author(s):  
Daisuke Kosemura ◽  
Munehisa Takei ◽  
Kohki Nagata ◽  
Hiroaki Akamatsu ◽  
Ryosuke Shimidzu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Numerical Aperture ◽  
Biaxial Stress ◽  
Objective Lens ◽  
High Numerical Aperture

scholarly journals Optical Projection Tomography Using a Commercial Microfluidic System

Micromachines ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 293
Author(s):  
Wenhao Du ◽  
Cheng Fei ◽  
Junliang Liu ◽  
Yongfu Li ◽  
Zhaojun Liu ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Numerical Aperture ◽  
Microfluidic System ◽  
Depth Of Field ◽  
Objective Lens ◽  
Flow Mode ◽  
Wide Field ◽  
Optical Projection Tomography ◽  
Microfluidic Systems ◽  
Optical Projection

Optical projection tomography (OPT) is the direct optical equivalent of X-ray computed tomography (CT). To obtain a larger depth of field, traditional OPT usually decreases the numerical aperture (NA) of the objective lens to decrease the resolution of the image. So, there is a trade-off between sample size and resolution. Commercial microfluidic systems can observe a sample in flow mode. In this paper, an OPT instrument is constructed to observe samples. The OPT instrument is combined with commercial microfluidic systems to obtain a three-dimensional and time (3D + T)/four-dimensional (4D) video of the sample. “Focal plane scanning” is also used to increase the images’ depth of field. A series of two-dimensional (2D) images in different focal planes was observed and compared with images simulated using our program. Our work dynamically monitors 3D OPT images. Commercial microfluidic systems simulate blood flow, which has potential application in blood monitoring and intelligent drug delivery platforms. We design an OPT adaptor to perform OPT on a commercial wide-field inverted microscope (Olympusix81). Images in different focal planes are observed and analyzed. Using a commercial microfluidic system, a video is also acquired to record motion pictures of samples at different flow rates. To our knowledge, this is the first time an OPT setup has been combined with a microfluidic system.

scholarly journals Three-dimensional holographic optical manipulation through a high-numerical-aperture soft-glass multimode fibre

2017 ◽  
Vol 12 (1) ◽  
pp. 33-39 ◽  
Author(s):  
Ivo T. Leite ◽  
Sergey Turtaev ◽  
Xin Jiang ◽  
Martin Šiler ◽  
Alfred Cuschieri ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Numerical Aperture ◽  
Optical Manipulation ◽  
High Numerical Aperture ◽  
Soft Glass
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