Pulsed optoacoustic interaction in suspension of gold nanoparticles: detection sensitivity based on laser-induced nanobubbles

2005 ◽  
Author(s):  
Sergey V. Egerev ◽  
Oleg B. Ovchinnikov ◽  
Andrey V. Fokin ◽  
Vasily V. Klimov ◽  
Dmitri Huzatau ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Detection Sensitivity ◽  
Optoacoustic Interaction ◽  
Nanoparticles Detection

scholarly journals Ultrasensitive and label free electrochemical immunosensor for detection of ROR1 as an oncofetal biomarker using gold nanoparticles assisted LDH/rGO nanocomposite

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rozita Abolhasan ◽  
Balal Khalilzadeh ◽  
Hadi Yousefi ◽  
Sahar Samemaleki ◽  
Forough Chakari-Khiavi ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Flexible Structures ◽  
Modified Electrodes ◽  
Electrochemical Immunosensor ◽  
Lymphocytic Leukemia ◽  
Orphan Receptor ◽  
Detection Sensitivity ◽  
Label Free ◽  
Serum Samples ◽  
Limit Of Quantification

AbstractIn the present article, we developed a highly sensitive label-free electrochemical immunosensor based on NiFe-layered double hydroxides (LDH)/reduced graphene oxide (rGO)/gold nanoparticles modified glassy carbon electrode for the determination of receptor tyrosine kinase-like orphan receptor (ROR)-1. In this electrochemical immunoassay platform, NiFe-LDH/rGO was used due to great electron mobility, high specific surface area and flexible structures, while Au nanoparticles were prepared and coated on the modified electrodes to improve the detection sensitivity and ROR1 antibody immobilizing (ROR1Ab). The modification procedure was approved by using cyclic voltammetry and differential pulse voltammetry based on the response of peak current to the step by step modifications. Under optimum conditions, the experimental results showed that the immunosensor revealed a sensitive response to ROR1 in the range of 0.01–1 pg mL−1, and with a lower limit of quantification of 10 attogram/mL (10 ag mL−1). Furthermore, the designed immunosensor was applied for the analysis of ROR1 in several serum samples of chronic lymphocytic leukemia suffering patients with acceptable results, and it also exhibited good selectivity, reproducibility and stability.

Simulation of Gold Nanoparticles Aggravating MEMS Cantilever Optical Static Detection Biochip

2013 ◽  
Vol 694-697 ◽  
pp. 966-970 ◽  
Author(s):  
Yue Tao Ge ◽  
Xiao Tong Yin
Keyword(s):  
Gold Nanoparticles ◽  
Low Cost ◽  
Mechanical Systems ◽  
Side Wall ◽  
High Sensitivity ◽  
Detection Sensitivity ◽  
Gene Detection ◽  
Micro Electro Mechanical Systems ◽  
Target Dna ◽  
Static Detection

A kind of gene detection biochip model based on biological micro electro mechanical systems (BioMEMS) technology and micro optical electro mechanical systems (MOEMS) technology is designed and simulated. In order to detect whether there are nucleic acid components in the testing samples, the biochip in this study issues horizontal light by laser, then receives and reads the deformation signals of MEMS cantilever by optical detector. The MEMS optical reflecting system can amplify MEMS cantilever deformation signal 22 times by micro reflectors which are set on the side wall of the cantilever free end. In order to improve optical detection sensitivity, gold nanoparticles (GNPs) which are combined with hybridization information is taken to aggravate MEMS cantilever, and employ Au - S chemical bond of GNPs and dithiol HS(CH2)6SH to combine and fix DNA probe, and then employ target DNA which is marked with biotin to combine GNPs by Biotin - Streptavidin combining. The simulation results show that this biochip can detect biological samples fast, high throughput, low cost, high sensitivity and reliably.

Polyelectrolytes-coated gold nanoparticles detection by PEDOT:PSS electrochemical transistors

2012 ◽  
Vol 13 (9) ◽  
pp. 1716-1721 ◽  
Author(s):  
T. Toccoli ◽  
E. Borga ◽  
H. Blond ◽  
D. Maniglio ◽  
L. Minati ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Nanoparticles Detection

Optical Properties and Detection Sensitivity of Noble Metal Nanoparticles

2018 ◽  
Vol 10 (3) ◽  
pp. 346-350
Author(s):  
Hongyan Zhao ◽  
Xin Liu ◽  
Shoubao Zhang ◽  
Xiaoli Liu ◽  
Rende Ma ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Silver Nanoparticles ◽  
Refractive Index ◽  
Extinction Spectrum ◽  
Spectral Characteristics ◽  
Resonance Wavelength ◽  
Detection Sensitivity ◽  
Noble Metal Nanoparticles ◽  
Localized Surface Plasmon ◽  
Refractive Indices

By using the finite difference time domain method, the localized surface plasmon extinction spectrum of different sizes of spherical gold and silver nanoparticles were simulated and analyzed in external environments with various refractive indices. It was observed that the changes in the position of the resonance wavelength were linearly proportional to the external refractive index, while the sensitivity increased with nanoparticle size. The sensitivity of the spherical silver nanoparticles with a radius of 40 nm reached 122 nm/RIU. For the silver and gold nanoparticles of the same size, the sensitivity of the silver nanoparticles was greater than that of the gold nanoparticles. In the experiment, the spectral characteristics of the silver nanoparticles with different sizes under various external refractive indices were measured. The experimental results were consistent with the simulation results. The resonance wavelength and the concentration of the solution showed a good linear relationship. By using the sensitive properties of the nanoparticles such as external refractive index, the amount of sucrose in the solution can be measured.

scholarly journals Gold Nanobeads with Enhanced Absorbance for Improved Sensitivity in Competitive Lateral Flow Immunoassays

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1488
Author(s):  
Xirui Chen ◽  
Xintao Miao ◽  
Tongtong Ma ◽  
Yuankui Leng ◽  
Liangwen Hao ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Detection Limit ◽  
Inhibitory Concentration ◽  
Fumonisin B1 ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Promising Strategy ◽  
Qualitative Detection ◽  
Better Than

Background: Colloidal gold based lateral flow immunoassay (LFIA) commonly suffers from relatively low detection sensitivity due to the insufficient brightness of conventional gold nanoparticles (AuNPs) with the size of 20–40 nm. Methods: Herein, three kinds of gold nanobeads (GNBs) with the size of 94 nm, 129 nm, and 237 nm, were synthesized by encapsulating numerous hydrophobic AuNPs (10 nm) into polymer matrix. The synthesized GNBs exhibited the enhanced colorimetric signal intensity compared with 20–40 nm AuNPs. The effects of the size of GNBs on the sensitivity of LFIA with competitive format were assessed. Results: The results showed that the LFIA using 129 nm GNBs as amplified signal probes exhibits the best sensitivity for fumonisin B1 (FB1) detection with a cut-off limit (for visual qualitative detection) at 125 ng/mL, a half maximal inhibitory concentration at 11.27 ng/mL, and a detection limit at 1.76 ng/mL for detection of real corn samples, which are 8-, 3.82-, and 2.89-fold better than those of conventional AuNP40-based LFIA, respectively. The developed GNB-LFIA exhibited negligible cross-reactions with other common mycotoxins. In addition, the accuracy, precision, reliability, and practicability were demonstrated by determining real corn samples. Conclusions: All in all, the proposed study provides a promising strategy to enhance the sensitivity of competitive LFIA via using the GNBs as amplified signal probes.

scholarly journals Image Digitization of Colorimetric Detection of Acephate Based on Its Complexation with Citrate-Capped Gold Nanoparticles

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Mohd Junaedy Osman ◽  
Wan Md Zin Wan Yunus ◽  
Keat Khim Ong ◽  
Buong Woei Chieng ◽  
Noor Azilah Mohd Kassim ◽  
...  
Keyword(s):  
Image Processing ◽  
Gold Nanoparticles ◽  
Limit Of Detection ◽  
Colorimetric Detection ◽  
Linear Regression Analysis ◽  
Processing Technique ◽  
Detection Sensitivity ◽  
Image Processing Technique ◽  
Detection Accuracy ◽  
Colour Image

Acephate (Ac), an organophosphate (OP) insecticide, is very harmful to human and the environment. Conventional techniques of detection are sensitive and selective but relatively time-consuming, expensive, and require trained personnel. This paper describes the use of an image processing technique to digitize the red values (RVs) of the colour image of Ac-capped gold nanoparticles (Ac-Cit-AuNPs) complex captured using a digital microscope to improve the detection accuracy and precision. The formation of the suspension was characterised using laser test, ultraviolet-visible spectrophotometer (UV-Vis), high-resolution transmission electron microscope (HRTEM), and Fourier Transform infrared spectroscope (FTIR). The linear regression analysis revealed that the detection sensitivity improved as the smaller gold nanoparticles were used. For quantitative measurement using image processing, a good linear relationship (R2 = 0.9905 and 0.9924) for Cit-HAuNPs and Cit-MAuNPs, respectively, between the concentration of Ac and average red values was obtained in the range of 0–8 mM. The limit of detection (LOD) for Ac was found to be 0.3 mM and 0.4 mM for Cit-MAuNPs and Cit-HAuNPs, respectively.

Nanogold Aggravating MEMS Cantilever Capacitance Detection Biochip

2010 ◽  
Vol 159 ◽  
pp. 429-433 ◽  
Author(s):  
Zhong Liang Deng ◽  
Yue Tao Ge ◽  
Wei Guo Guan ◽  
Nai Bo Zhang ◽  
Qi Ke Cao
Keyword(s):  
Gold Nanoparticles ◽  
Integrated Circuit ◽  
Low Cost ◽  
High Sensitivity ◽  
Detection Sensitivity ◽  
Capacitive Pressure Sensor ◽  
Target Dna ◽  
Capacitance Detection ◽  
Before And After ◽  
Hybridization Reaction

A kind of array micro-electromechanical systems (MEMS) cantilever of biochip is designed, which integrated capacitive pressure sensor. Before and after hybridization reaction, by the change of capacitance value, it can measure the capacitance values through integrated circuit (IC) to judge whether the solution containing the cantilever probe genes. In order to improve the detection sensitivity, it aggravate cantilever by gold nanoparticles combining hybridization information, applying Au-S chemical bond of gold nanoparticles and dithiol HS(CH2)6SH to combine and fix DNA probes and applying target DNA marked with biotin to combine gold nanoparticles by Biotin - Streptavidin combining. The results shows that this biochip can detect biological samples fast, high throughput, low cost, high sensitivity and reliably.

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