Shape change analysis of confocal microscope images using variational techniques

1992 ◽  
Author(s):  
Keith A. Bartels ◽  
Alan C. Bovik ◽  
Shanti J. Aggarwal ◽  
Kenneth R. Diller
Keyword(s):  
Shape Change ◽  
Confocal Microscope ◽  
Change Analysis ◽  
Variational Techniques ◽  
Microscope Images

scholarly journals Optimal Multiresolution Blending of Confocal Microscope Images

2012 ◽  
Vol 59 (2) ◽  
pp. 531-541 ◽  
Author(s):  
Hao-Chiang Shao ◽  
Wen-Liang Hwang ◽  
Yung-Chang Chen
Keyword(s):  
Confocal Microscope ◽  
Microscope Images

Multiresolution analysis of 2D confocal microscope images

2010 ◽  
Author(s):  
D. Bianchi ◽  
A. Vernes ◽  
G. Vorlaufer ◽  
G. Betz
Keyword(s):  
Multiresolution Analysis ◽  
Confocal Microscope ◽  
Microscope Images

scholarly journals Segmentation of confocal microscope images of cell nuclei in thick tissue sections

1999 ◽  
Vol 193 (3) ◽  
pp. 212-226 ◽  
Author(s):  
C. ORTIZ DE SOLORZANO ◽  
E. GARCIA RODRIGUEZ ◽  
A. JONES ◽  
D. PINKEL ◽  
J. W. GRAY ◽  
...  
Keyword(s):  
Confocal Microscope ◽  
Cell Nuclei ◽  
Tissue Sections ◽  
Microscope Images

scholarly journals Automated image analysis methods for 3-D quantification of the neurovascular unit from multichannel confocal microscope images

2005 ◽  
Vol 66A (1) ◽  
pp. 9-23 ◽  
Author(s):  
Gang Lin ◽  
Chris S. Bjornsson ◽  
Karen L. Smith ◽  
Muhammad-Amri Abdul-Karim ◽  
James N. Turner ◽  
...  
Keyword(s):  
Image Analysis ◽  
Neurovascular Unit ◽  
Confocal Microscope ◽  
Automated Image Analysis ◽  
Analysis Methods ◽  
Microscope Images

scholarly journals Shape change analysis of tensegrity models

2019 ◽  
Vol 16 (7) ◽  
Author(s):  
Chai Lian Oh ◽  
Kok Keong Choong ◽  
Toku Nishimura ◽  
Jae-Yeol Kim ◽  
Omid Hassanshahi
Keyword(s):  
Shape Change ◽  
Change Analysis

scholarly journals Cell migration through three-dimensional confining pores: speed accelerations by deformation and recoil of the nucleus

2019 ◽  
Vol 374 (1779) ◽  
pp. 20180225 ◽  
Author(s):  
Marina Krause ◽  
Feng Wei Yang ◽  
Mariska te Lindert ◽  
Philipp Isermann ◽  
Jan Schepens ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Migration ◽  
Shape Change ◽  
Three Dimensional ◽  
Time Lapse ◽  
Cell Cycle Phase ◽  
Cycle Phase ◽  
Change Analysis ◽  
Shape Adaptation ◽  
Interdisciplinary Approaches

Directional cell migration in dense three-dimensional (3D) environments critically depends upon shape adaptation and is impeded depending on the size and rigidity of the nucleus. Accordingly, the nucleus is primarily understood as a physical obstacle; however, its pro-migratory functions by stepwise deformation and reshaping remain unclear. Using atomic force spectroscopy, time-lapse fluorescence microscopy and shape change analysis tools, we determined the nuclear size, deformability, morphology and shape change of HT1080 fibrosarcoma cells expressing the Fucci cell cycle indicator or being pre-treated with chromatin-decondensating agent TSA. We show oscillating peak accelerations during migration through 3D collagen matrices and microdevices that occur during shape reversion of deformed nuclei (recoil), and increase with confinement. During G1 cell-cycle phase, nucleus stiffness was increased and yielded further increased speed fluctuations together with sustained cell migration rates in confinement when compared to interphase populations or to periods of intrinsic nuclear softening in the S/G2 cell-cycle phase. Likewise, nuclear softening by pharmacological chromatin decondensation or after lamin A/C depletion reduced peak oscillations in confinement. In conclusion, deformation and recoil of the stiff nucleus contributes to saltatory locomotion in dense tissues. This article is part of a discussion meeting issue ‘Forces in cancer: interdisciplinary approaches in tumour mechanobiology’.

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