Laser flash photolysis with real-time mass spectrometry detection

2004 ◽  
Author(s):  
J. Codnia ◽  
Francisco A. Manzano ◽  
M. L. Azcarate
Keyword(s):  
Mass Spectrometry ◽  
Real Time ◽  
Flash Photolysis ◽  
Laser Flash Photolysis ◽  
Laser Flash ◽  
Mass Spectrometry Detection

scholarly journals Measurements of Rate Constants for HO2 + NO and NH2 + NO Reactions by Time-Resolved Photoionization Mass Spectrometry

1995 ◽  
Vol 16 (1) ◽  
pp. 43-51 ◽  
Author(s):  
Takashi Imamura ◽  
Nobuaki Washida
Keyword(s):  
Mass Spectrometry ◽  
Rate Constants ◽  
Flash Photolysis ◽  
Laser Flash Photolysis ◽  
Laser Flash ◽  
Absolute Rate ◽  
Time Resolved ◽  
Photoionization Mass Spectrometry

Absolute rate constants for HO2 + NO and NH2 + NO reactions were measured by a photoionization mass spectrometry coupled with a laser flash photolysis. HO2 and NH2 radicals were photoionized by an Ar resonance lamp and were detected as their parent ions (HO2+ and NH2+). The rate constants were determined to be K(HO2 + NO)=(6.5  ± 2.0) 10−12cm3molecule−1s−1K(NH2 + NO)=(1.9  ± 0.3) 10−11cm3molecule−1s−1 Both rates are consistent with those previously reported.

scholarly journals Intrinsic Buffer Hydroxyl Radical Dosimetry Using Tris(Hydroxymethyl)Aminomethane

2019 ◽  
Author(s):  
Addison E. Roush ◽  
Mohammad Riaz ◽  
Sandeep K. Misra ◽  
Scot R. Weinberger ◽  
Joshua S. Sharp
Keyword(s):  
Hydroxyl Radical ◽  
Real Time ◽  
Hydroxyl Radicals ◽  
Flash Photolysis ◽  
Laser Flash Photolysis ◽  
Laser Flash ◽  
Photochemical Oxidation ◽  
Protein Surfaces ◽  
Radical Production ◽  
Experimental Parameters

AbstractFast Photochemical Oxidation of Proteins (FPOP) is a powerful covalent labeling tool that uses hydroxyl radicals generated by laser flash photolysis of hydrogen peroxide to footprint protein surfaces. Because radical production varies with many experimental parameters, hydroxyl radical dosimeters have been introduced to track the effective radical dosage experienced by the protein analyte. FPOP experiments performed using adenine optical radical dosimetry containing protein in Tris buffer demonstrated unusual dosimetry behavior. We have investigated the behavior of Tris under oxidative conditions in detail. We find that Tris can act as a novel gain-of-signal optical hydroxyl radical dosimeter in FPOP experiments. This new dosimeter is also amenable to inline real-time monitoring thereby allowing real-time adjustments to compensate for differences in samples for their quenching ability.

scholarly journals Early Events of Photosensitized Oxidation of Sulfur-Containing Amino Acids Studied by Laser Flash Photolysis and Mass Spectrometry

2020 ◽  
Vol 124 (35) ◽  
pp. 7564-7573
Author(s):  
Tomasz Pedzinski ◽  
Katarzyna Grzyb ◽  
Franciszek Kaźmierczak ◽  
Rafał Frański ◽  
Piotr Filipiak ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Flash Photolysis ◽  
Laser Flash Photolysis ◽  
Laser Flash ◽  
Photosensitized Oxidation ◽  
Sulfur Containing

Effect of Aggregation on Bacteriochlorin a Triplet-state Formation: A Laser Flash Photolysis Study¶

2002 ◽  
Vol 76 (5) ◽  
pp. 480 ◽  
Author(s):  
Xavier Damoiseau ◽  
Francis Tfibel ◽  
Maryse Hoebeke ◽  
Marie-Pierre Fontaine-Aupart
Keyword(s):  
Triplet State ◽  
State Formation ◽  
Flash Photolysis ◽  
Laser Flash Photolysis ◽  
Laser Flash

A Laser Flash Photolysis and Pulse Radiolysis Study of Primary Photochemical Processes of Flumequine¶

2000 ◽  
Vol 72 (4) ◽  
pp. 451 ◽  
Author(s):  
M. Bazin ◽  
F. Bosca ◽  
M. L. Marin ◽  
M. A. Miranda ◽  
L. K. Patterson ◽  
...  
Keyword(s):  
Pulse Radiolysis ◽  
Flash Photolysis ◽  
Laser Flash Photolysis ◽  
Laser Flash ◽  
Photochemical Processes
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