Perturbations of the local environment of the single tyrosine residue in neurophysin upon ligand binding as determined by steady-state and time-resolved fluorescence quenching

1992 ◽  
Author(s):  
Carol A. Hasselbacher ◽  
Zhen H. Shen ◽  
William R. Laws
Keyword(s):  
Steady State ◽  
Fluorescence Quenching ◽  
Ligand Binding ◽  
Local Environment ◽  
Tyrosine Residue ◽  
Time Resolved Fluorescence ◽  
Time Resolved

scholarly journals Analysis of Fluorescence Quenching of BPBD by Aniline in Toluene

2013 ◽  
Vol 12 (1) ◽  
pp. 87-98
Author(s):  
J Thipperudrappa ◽  
S M Hanagodimath
Keyword(s):  
Steady State ◽  
Fluorescence Quenching ◽  
Room Temperature ◽  
Static Quenching ◽  
Approximation Model ◽  
Positive Deviation ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Steady State Method ◽  
Volmer Plot

Fluorescence quenching of 2-(4'-t-Butylphenyl)-5-(4"-biphenylyl)-1,3,4-oxadiazole (BPBD) by aniline in toluene has been carried out at room temperature by steady state and time resolved fluorescence spectroscopy. The Stern-Volmer plot by steady state method has been found to be non-linear showing a positive deviation, whereas by time-resolved method it is linear. In order to interpret these results we have used the ground state complex and sphere of action static quenching models. Using these models various rate parameters have been determined. Based on these models, with finite sink approximation model, we conclude that positive deviation Stern-Volmer plot is due to the simultaneous presence of dynamic and static quenching processes.

scholarly journals Binding of Calcium and Magnesium Ions to Terrestrial Chromophoric Dissolved Organic Matter (CDOM): A Combination of Steady-State and Time-Resolved Fluorescence Study

Water ◽  
2021 ◽  
Vol 13 (16) ◽  
pp. 2182
Author(s):  
Juan Liu ◽  
Ruiya Zhou ◽  
Xu Zhang
Keyword(s):  
Organic Matter ◽  
Steady State ◽  
Fluorescence Quenching ◽  
Dissolved Organic Matter ◽  
Fluorescence Decay ◽  
Chromophoric Dissolved Organic Matter ◽  
Time Resolved Fluorescence ◽  
High Concentration ◽  
Time Resolved ◽  
Order Of Magnitude

Revealing the binding properties of calcium ion (Ca2+) and magnesium ion (Mg2+) to terrestrial chromophoric dissolved organic matter (CDOM) facilities understanding the effect of natural water components on the photophysics of dissolved organic matter. Steady-state and time-resolved fluorescence spectrometry, and dynamic light scattering were applied to investigate the fluorescence quenching process of CDOM by Ca2+ and Mg2+. Due to a remarkable decrease of the steady-state fluorescence intensity and a slight decrease of fluorescence lifetime, the fluorescence quenching of CDOM by cations mainly occurred through a static process. The fluorescence quenching was profound under longer excitation and emission wavelengths. The binding constant (K, L/mol) for Ca2+ to CDOM ranged from 4.29 to 5.09 (lgK), which was approximately one order of magnitude higher than that of Mg2+ to CDOM (3.86 to 4.56). Nevertheless, the efficiency of CDOM fluorescence quenching by Ca2+, Mg2+ was much lower than that by Cu2+. Fluorescence decay became faster in the presence of a high concentration of Ca2+ (>20 mg/L) and Mg2+ (>50 mg/L). In the presence of these two metal ions, particularly for Ca2+, the lifetime of CDOM excited states shifted to the relatively small value side, indicating fluorescence quenching of CDOM mainly occurred through the interaction of Ca2+/Mg2+ with relatively long-lived fluorophores.

Probing electron transfer dynamics of pyranine with reduced graphene oxide

2014 ◽  
Vol 16 (38) ◽  
pp. 20878-20886 ◽  
Author(s):  
M. Asha Jhonsi ◽  
C. Nithya ◽  
A. Kathiravan
Keyword(s):  
Electron Transfer ◽  
Graphene Oxide ◽  
Steady State ◽  
Fluorescence Quenching ◽  
Reduced Graphene Oxide ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Reduced Graphene ◽  
Fluorescence Quenching Method ◽  
Quenching Method

Electron transfer dynamics of POH with rGO has been investigated by using steady state and time-resolved fluorescence quenching method.

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