Correlation of conformational heterogeneity of the tryptophyl side chain and time-resolved fluorescence intensity decay kinetics

1992 ◽  
Author(s):  
William R. Laws ◽  
J. B. Alexander Ross
Keyword(s):  
Fluorescence Intensity ◽  
Side Chain ◽  
Conformational Heterogeneity ◽  
Time Resolved Fluorescence ◽  
Decay Kinetics ◽  
Time Resolved ◽  
Fluorescence Intensity Decay ◽  
Intensity Decay

scholarly journals Time-resolved and temperature tuneable measurements of fluorescent intensity using a smartphone fluorimeter

The Analyst ◽  
2017 ◽  
Vol 142 (11) ◽  
pp. 1953-1961 ◽  
Author(s):  
Md Arafat Hossain ◽  
John Canning ◽  
Zhikang Yu ◽  
Sandra Ast ◽  
Peter J. Rutledge ◽  
...  
Keyword(s):  
Steady State ◽  
Fluorescence Intensity ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Fluorescent Intensity ◽  
Intensity Measurements

A smartphone fluorimeter is demonstrated for steady-state and time-resolved fluorescence intensity measurements at tunable temperatures.

Characterization of Plasma-Modified Fluoropolymer Surfaces Using Steady-State and Time-Resolved Fluorescence Spectroscopy

1994 ◽  
Vol 48 (5) ◽  
pp. 630-637 ◽  
Author(s):  
Ming Li ◽  
Michaeleen L. Pacholski ◽  
Frank V. Bright
Keyword(s):  
Steady State ◽  
Fluorescence Spectroscopy ◽  
Sulfonyl Chloride ◽  
Sodium Dodecylsulfate ◽  
Covalent Immobilization ◽  
Ambient Conditions ◽  
Time Resolved Fluorescence ◽  
Decay Kinetics ◽  
Time Resolved

Poly(hexafluoropropylene-co-tetrafluoroethylene) (FEP) has been widely used in biotechnology because of its unique surface properties and biocompatibility. Recent work from our group has shown that plasma discharge-modified FEP can be used as the substratum for development of a very stable immunosensor. This result has prompted us to study further this new surface under ambient conditions. In this paper, we report on the covalent immobilization of a pyrene residue (-Py) onto FEP-APS (FEP-aminopropyl silane) surfaces and the characterization of FEP-APS-Py using steady-state and time-resolved fluorescence spectroscopy. Among the immobilization schemes tested, we found that the covalent coupling of pyrene-sulfonyl chloride to FEP-APS is the easiest and yields the most photostable FEP-APS-Py derivative. Steady-state emission spectra of FEP-APS-Py in contact with H2O, β-cyclodextrin (β-CD), and sodium dodecylsulfate (SDS) aqueous solutions differ considerably from those of Py-SO3 in solution. Time-resolved fluorescence spectroscopy of FEP-APS-Py demonstrates that the decay kinetics are strongly affected by the presence of ionic quenchers and molecular oxygen, as well as β-CD and SDS. The results are consistent with the suggestion that the APS-Py moiety undergoes a slow time-dependent reconfiguration at the FEP/APS interface.

scholarly journals Fluorescence intensity decay shape analysis microscopy (FIDSAM) for quantitative and sensitive live-cell imaging

2010 ◽  
Author(s):  
Sébastien Peter ◽  
Kirstin Elgass ◽  
Marcus Sackrow ◽  
Katharina Caesar ◽  
Anne-Kathrin Born ◽  
...  
Keyword(s):  
Shape Analysis ◽  
Fluorescence Intensity ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Fluorescence Intensity Decay ◽  
Intensity Decay
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