Surface-enhanced Raman spectroscopy in the structural studies of biomolecules: the state of the art

1991 ◽  
Author(s):  
Igor R. Nabiev ◽  
Konstantin V. Sokolov ◽  
R. G. Efremov ◽  
George D. Chumanov
Keyword(s):  
Raman Spectroscopy ◽  
State Of The Art ◽  
Surface Enhanced Raman Spectroscopy ◽  
The State ◽  
Structural Studies ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Nanomedical Applications of Optofluidics

2010 ◽  
Author(s):  
David Erickson
Keyword(s):  
Raman Spectroscopy ◽  
Single Molecule ◽  
State Of The Art ◽  
Sensor Arrays ◽  
Surface Enhanced Raman Spectroscopy ◽  
Detection Sensitivity ◽  
Optical Forces ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Single Molecule Analysis

In this talk I will discuss some of the nanomedical applications of optofluidic systems. As will be demonstrated such devices hold significant promise for improving on the state of the art in detection sensitivity as well as enable entirely new modalities for molecular analysis. Two example platforms will be discussed. The first of these will be our Nanoscale Optofluidic Sensor Arrays which comprise of a series of 1D evanescently coupled linear optical resonators. In addition to demonstrating both nucleic acid (Dengue virus) and immunological detection (Interleukins), I will show how optical forces can be used to increase the functionality of these devices including single molecule analysis. The second platform described is a method for performing surface enhanced raman spectroscopy (SERS) on a chip. A unique ligase detection reaction based assay will be demonstrated to show the unique advantages of the approach.

Bacterial detection and differentiation via direct volatile organic compound sensing with surface enhanced Raman spectroscopy

2017 ◽  
Author(s):  
Caitlin S. DeJong ◽  
David I. Wang ◽  
Aleksandr Polyakov ◽  
Anita Rogacs ◽  
Steven J. Simske ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Direct Detection ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
E Coli ◽  
Recent Emergence ◽  
Surface Enhanced ◽  
Volatile Organic ◽  
Surface Enhanced Raman

Through the direct detection of bacterial volatile organic compounds (VOCs), via surface enhanced Raman spectroscopy (SERS), we report here a reconfigurable assay for the identification and monitoring of bacteria. We demonstrate differentiation between highly clinically relevant organisms: <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Serratia marcescens</i>. This is the first differentiation of bacteria via SERS of bacterial VOC signatures. The assay also detected as few as 10 CFU/ml of <i>E. coli</i> in under 12 hrs, and detected <i>E. coli</i> from whole human blood and human urine in 16 hrs at clinically relevant concentrations of 10<sup>3</sup> CFU/ml and 10<sup>4</sup> CFU/ml, respectively. In addition, the recent emergence of portable Raman spectrometers uniquely allows SERS to bring VOC detection to point-of-care settings for diagnosing bacterial infections.

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