Simultaneous SPR and electrochemical sensing of an alkane-thiol self-assembled monolayer (SAM): toward an optical biosensor

2004 ◽  
Author(s):  
A. K. Sheridan ◽  
P. Ngamukot ◽  
Philip N. Bartlett ◽  
James S. Wilkinson
Keyword(s):  
Optical Biosensor ◽  
Electrochemical Sensing ◽  
Self Assembled Monolayer ◽  
Alkane Thiol ◽  
Self Assembled

scholarly journals Vapor-phase deposition-based self-assembled monolayer for an electrochemical sensing platform

AIP Advances ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 045213
Author(s):  
Jun Ki Ahn ◽  
Seung Jun Oh ◽  
Hun Park ◽  
Yesol Song ◽  
Seong Jung Kwon ◽  
...  
Keyword(s):  
Vapor Phase ◽  
Electrochemical Sensing ◽  
Self Assembled Monolayer ◽  
Vapor Phase Deposition ◽  
Sensing Platform ◽  
Self Assembled

THE KINETICS OF THE AS GROWN AND ANNEALED SELF-ASSEMBLED MONOLAYER STUDIED BY FORCE SPECTROSCOPY

2018 ◽  
Vol 25 (02) ◽  
pp. 1850054
Author(s):  
HUMA HABIB ◽  
M. YASAR ◽  
S. MEHMOOD ◽  
SAIMA RAFIQUE ◽  
A. S. BHATTI ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Self Assembly ◽  
Surface Coverage ◽  
Work Of Adhesion ◽  
Self Assembled Monolayer ◽  
Force Microscopy ◽  
Atomic Force ◽  
Alkane Thiol ◽  
Self Assembled ◽  
Kinetics Of

The growth of biological systems like DNA, peptides and proteins are accredited to the self-assembly processes from the molecular level to the nanoscale. The flawless immobilization of DNA on any surface is quite an important step to the development of DNA-based biosensors. The present paper reports the use of atomic force microscopy to determine the mechanical properties of the as grown and annealed self-assembled monolayer (SAM) as well as the mutated DNA immobilized on the SAM. The SAM of alkane thiol (16-mercapto-1-hexadecanol) was developed on Au surface, which was then annealed and analyzed for its structural and mechanical properties. The surface coverage, height and monolayer’s order was studied as a function of incubation time and annealing time. Excessive annealing led to the defragmentation and desorption of SAM structures due to breaking of hydrocarbon bonds. AFM was employed to determine the detach separation, pull-off and work of adhesion of the as grown and annealed SAM.

Hemin Conjugated Self Assembled Monolayer on Gold Nanoparticles in Polypyrrole Matrix for Nitrite Electrochemical Sensing

2017 ◽  
Vol 15 (7) ◽  
pp. 608-615
Author(s):  
Paulraj Santharaman ◽  
Arya Aditya ◽  
Chhabra Aastha ◽  
Niroj K. Sethy ◽  
Kalpana Bhargava ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Electrochemical Sensing ◽  
Self Assembled Monolayer ◽  
Self Assembled

scholarly journals Impact of Self-Assembled Monolayer Design and Electrochemical Factors on Impedance-Based Biosensing

Sensors ◽  
2020 ◽  
Vol 20 (8) ◽  
pp. 2246 ◽  
Author(s):  
Michael C. Brothers ◽  
David Moore ◽  
Michael St. Lawrence ◽  
Jonathan Harris ◽  
Ronald M. Joseph ◽  
...  
Keyword(s):  
Charge Density ◽  
Real Time ◽  
Electrostatic Interactions ◽  
Electrochemical Sensing ◽  
Single Frequency ◽  
Self Assembled Monolayers ◽  
Self Assembled Monolayer ◽  
Frequency Measurements ◽  
Assembled Monolayers ◽  
Self Assembled

Real-time sensing of proteins, especially in wearable devices, remains a substantial challenge due to the need to convert a binding event into a measurable signal that is compatible with the chosen analytical instrumentation. Impedance spectroscopy enables real-time detection via either measuring electrostatic interactions or electron transfer reactions while simultaneously being amenable to miniaturization for integration into wearable form-factors. To create a more robust methodology for optimizing impedance-based sensors, additional fundamental studies exploring components influencing the design and implementation of these sensors are needed. This investigation addresses a sub-set of these issues by combining optical and electrochemical characterization to validate impedance-based sensor performance as a function of (1) biorecognition element density, (2) self-assembled monolayer chain length, (3) self-assembled monolayer charge density, (4) the electrochemical sensing mechanism and (5) the redox reporter selection. Using a pre-existing lysozyme aptamer and lysozyme analyte combination, we demonstrate a number of design criteria to advance the state-of-the-art in protein sensing. For this model system we demonstrated the following: First, denser self-assembled monolayers yielded substantially improved sensing results. Second, self-assembled monolayer composition, including both thickness and charge density, changed the observed peak position and peak current. Third, single frequency measurements, while less informative, can be optimized to replace multi-frequency measurements and in some cases (such as that with zwitterionic self-assembled monolayers) are preferred. Finally, various redox reporters traditionally not used in impedance sensing should be further explored. Collectively, these results can help limit bottlenecks associated with device development, enabling realization of next-generation impedance-based biosensing with customize sensor design for the specific application.

Self-Assembled Fluid Phase Floating Membranes with Tuneable Water Interlayers

2019 ◽  
Author(s):  
Luke Clifton ◽  
Nicoló Paracini ◽  
Arwel V. Hughes ◽  
Jeremy H. Lakey ◽  
Nina-Juliane Seinke ◽  
...  
Keyword(s):  
Fluid Phase ◽  
Interlayer Thickness ◽  
Supported Bilayers ◽  
Self Assembled Monolayer ◽  
High Coverage ◽  
Nano Technology ◽  
Surface Distance ◽  
Potential Applications ◽  
Self Assembled ◽  
Coated Surfaces

<p>We present a reliable method for the fabrication of fluid phase unsaturated bilayers which are readily self-assembled on charged self-assembled monolayer (SAM) surfaces producing high coverage floating supported bilayers where the membrane to surface distance could be controlled with nanometer precision. Vesicle fusion was used to deposit the bilayers onto anionic SAM coated surfaces. Upon assembly the bilayer to SAM solution interlayer thickness was 7-10 Å with evidence suggesting that this layer was present due to SAM hydration repulsion of the bilayer from the surface. This distance could be increased using low concentrations of salts which caused the interlayer thickness to enlarge to ~33 Å. Reducing the salt concentration resulted in a return to a shorter bilayer to surface distance. These accessible and controllable membrane models are well suited to a range of potential applications in biophysical studies, bio-sensors and Nano-technology.</p><br>

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