Automatic detection of intracellular nitric oxide concentration by confocal laser scanning microscopy images analysis

2003 ◽  
Author(s):  
John Kutor ◽  
Gang-min Ning ◽  
Di-hui Hong ◽  
Xiaoxiang Zheng
Keyword(s):  
Nitric Oxide ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Automatic Detection ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Oxide Concentration ◽  
Nitric Oxide Concentration ◽  
Microscopy Images

Synchronous detection of vascular tension and nitric oxide release in pulmonary artery: A combined application of confocal wire myograph with confocal laser scanning microscopy

Vascular ◽  
2020 ◽  
Vol 28 (5) ◽  
pp. 619-628
Author(s):  
Xiao-Ling Zhuang ◽  
Zhuang-Li Zhu ◽  
Jie-Ling Zhu ◽  
Su-Mei Lai ◽  
Long-Xin Gui ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Pulmonary Artery ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Combined Application ◽  
M 10 ◽  
No Release

Objectives To detect the vascular tension and nitric oxide (NO) release synchronously in mice pulmonary artery, we perform two experiments and present a novel application of confocal wire myograph coupled with the confocal laser scanning microscopy. Methods In the first experiment, viable endothelium-intact mouse pulmonary artery (outer diameter 100–300 μM) rings underwent a one-hour preincubation with a NO-specific fluorescent dye, 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate Calbiochem (2.5 μM), and then precontracted with phenylephrine (Phen, 10−6 M), and subsequently dilated in acetylcholine (ACh, 10−6 M – 10−4 M). The endothelium-dependent vasorelaxation and NO generation in pulmonary artery rings were simultaneously recorded. In the second experiment, after 30-min incubation with the former NO fluorescent dye, the qualified pulmonary artery rings were co-incubated for another 30 min with a nitric oxide synthase inhibitor, 10−4 M Nω-nitro-L-arginine-methyl-ester (L-NAME), and then pretreated with Phen (10−6 M) followed by ACh (10−5 M). The Ach-induced vasodilation and NO release were recorded simultaneously. Results ACh (10−6 M – 10−4 M) promoted pulmonary artery relaxation and intracellular NO release in a dose-dependent manner. Additionally, L-NAME (10−4 M) significantly attenuated the vasodilatation and the intracellular NO release. Conclusions This combined application visually confirms that the synchronous changes in Ach induced vasodilation and NO release, which provides a new method for cardiovascular research.

Estimating the motion of plant root cells from in vivo confocal laser scanning microscopy images

2009 ◽  
Vol 21 (6) ◽  
pp. 921-939 ◽  
Author(s):  
Timothy J. Roberts ◽  
Stephen J. McKenna ◽  
Cheng-Jin Du ◽  
Nathalie Wuyts ◽  
Tracy A. Valentine ◽  
...  
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Plant Root ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Root Cells ◽  
Microscopy Images
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