Effective axial superresolution in single-photon 4Pi microscopy by Toraldo rings

2003 ◽  
Author(s):  
Manuel Martinez-Corral ◽  
M. T. Caballero ◽  
Amparo Pons Marti
Keyword(s):  
Single Photon ◽  
4Pi Microscopy ◽  
Axial Superresolution

SUPPRESSION OF SIDELOBES IN SINGLE-PHOTON 4PI CONFOCAL MICROSCOPY BY FÖRSTER RESONANT ENERGY TRANSFER

2012 ◽  
Vol 05 (03) ◽  
pp. 1250016
Author(s):  
JIANFANG CHEN ◽  
YA CHENG
Keyword(s):  
Focal Spot ◽  
Single Photon ◽  
Optical Resolution ◽  
Axial Direction ◽  
Diffraction Limit ◽  
Fluorescent Label ◽  
The Novel ◽  
Dye Molecules ◽  
4Pi Microscopy ◽  
Co Doped

Recently, we theoretically demonstrate that utilization of silica nanobeads co-doped with Cy3 and Cy5 molecules instead of single dye molecules as fluorescent labels can enable optical resolutions far beyond the diffraction-limit. Here, we show that by combining the 4Pi microscopy and the novel fluorescent label, it is possible to completely suppress the sidelobes in 4Pi focal spot and significantly enhance the optical resolution in the axial direction.

Sidelobe decline in single-photon 4Pi microscopy by Toraldo rings

Micron ◽  
2003 ◽  
Vol 34 (6-7) ◽  
pp. 319-325 ◽  
Author(s):  
M. Martı́nez-Corral ◽  
M.T. Caballero ◽  
A. Pons ◽  
P. Andrés
Keyword(s):  
Single Photon ◽  
4Pi Microscopy
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