New methods for CD measurements on photomasks using dark field optical microscopy

2003 ◽  
Author(s):  
Bernd Bodermann ◽  
Winfried Michaelis ◽  
Alexander Diener ◽  
Werner Mirande
Keyword(s):  
Optical Microscopy ◽  
Dark Field ◽  
New Methods

scholarly journals Multiplexed Plasmonic Nano-Labeling for Bioimaging of Cytological Stained Samples

Cancers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 3509
Author(s):  
Paule Marcoux-Valiquette ◽  
Cécile Darviot ◽  
Lu Wang ◽  
Andrée-Anne Grosset ◽  
Morteza Hasanzadeh Kafshgari ◽  
...  
Keyword(s):  
Accurate Determination ◽  
Cell Types ◽  
Fine Needle Biopsy ◽  
Dark Field ◽  
Imaging Method ◽  
New Methods ◽  
Formalin Fixed Paraffin ◽  
Dark Field Microscopy ◽  
Formalin Fixed Paraffin Embedded

Reliable cytopathological diagnosis requires new methods and approaches for the rapid and accurate determination of all cell types. This is especially important when the number of cells is limited, such as in the cytological samples of fine-needle biopsy. Immunoplasmonic-multiplexed- labeling may be one of the emerging solutions to such problems. However, to be accepted and used by the practicing pathologists, new methods must be compatible and complementary with existing cytopathology approaches where counterstaining is central to the correct interpretation of immunolabeling. In addition, the optical detection and imaging setup for immunoplasmonic-multiplexed-labeling must be implemented on the same cytopathological microscope, not interfere with standard H&E imaging, and operate as a second easy-to-use imaging method. In this article, we present multiplex imaging of four types of nanoplasmonic markers on two types of H&E-stained cytological specimens (formalin-fixed paraffin embedded and non-embedded adherent cancer cells) using a specially designed adapter for SI dark-field microscopy. The obtained results confirm the effectiveness of the proposed optical method for quantitative and multiplex identification of various plasmonic NPs, and the possibility of using immunoplasmonic-multiplexed-labeling for cytopathological diagnostics.

Optics, Optical Methods, and Microscopy

2021 ◽  
pp. 345-407
Author(s):  
Kannan M. Krishnan
Keyword(s):  
Optical Microscopy ◽  
Polarization State ◽  
Three Dimensional ◽  
Polarized Light ◽  
Dark Field ◽  
Optical Methods ◽  
Transverse Waves ◽  
Dark Field Imaging ◽  
Propagation Of Light ◽  
Scanning Optical Microscopy

Propagation of light is described as the simple harmonic motion of transverse waves. Combining waves that propagate on orthogonal planes give rise to linear, elliptical, or spherical polarization, depending on their amplitudes and phase differences. Classical experiments of Huygens and Young demonstrated the principle of optical interference and diffraction. Generalization of Fraunhofer diffraction to scattering by a three-dimensional arrangement of atoms in crystals forms the basis of diffraction methods. Fresnel diffraction finds application in the design of zone plates for X-ray microscopy. Optical microscopy, with resolution given by the Rayleigh criterion to be approximately half the wavelength, works best when tailored to the optimal characteristics of the human eye (λ = 550 nm). Lenses suffer from spherical and chromatic aberrations, and astigmatism. Optical microscopes operate in bright-field, oblique, and dark-field imaging conditions, produce interference contrast, and can image with polarized light. Variants include confocal scanning optical microscopy (CSOM). Metallography, widely used to characterize microstructures, requires polished or chemically etched surfaces to provide optimal contrast. Finally, the polarization state of light reflected from the surface of a specimen is utilized in ellipsometry to obtain details of the optical properties and thickness of thin film materials.

Hyperspectral Dark Field Optical Microscopy of Single Silver Nanospheres

2016 ◽  
Vol 120 (13) ◽  
pp. 7295-7298 ◽  
Author(s):  
Patrick Z. El-Khoury ◽  
Alan G. Joly ◽  
Wayne P. Hess
Keyword(s):  
Optical Microscopy ◽  
Dark Field ◽  
Silver Nanospheres

Detection and study of magnetic micro-and nanostructures using dark-field optical microscopy

2003 ◽  
Vol 45 (3) ◽  
pp. 519-528 ◽  
Author(s):  
V. I. Belotelov ◽  
A. S. Logginov ◽  
A. V. Nikolaev
Keyword(s):  
Optical Microscopy ◽  
Dark Field

Evolution of gold nanoparticle clusters in living cells studied by sectional dark-field optical microscopy and chromatic analysis

2015 ◽  
Vol 9 (7) ◽  
pp. 738-749 ◽  
Author(s):  
Sheng-Hann Wang ◽  
Chia-Wei Lee ◽  
Fan-Gang Tseng ◽  
Kuo-Kan Liang ◽  
Pei-Kuen Wei
Keyword(s):  
Optical Microscopy ◽  
Gold Nanoparticle ◽  
Living Cells ◽  
Dark Field ◽  
Nanoparticle Clusters

scholarly journals Application of optical microscopy for the qualitative and quantitative analysis of the solid surface

2020 ◽  
Vol 20 (2) ◽  
pp. 41-64
Author(s):  
A.A. Dedkova ◽  
◽  
M.A. Makhiboroda ◽  
Keyword(s):  
Quantitative Analysis ◽  
Optical Microscopy ◽  
Surface Relief ◽  
Complex Analysis ◽  
Dark Field ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Surface Measurements ◽  
Defects Analysis

The possibilities of optical microscopy for preliminary and complex analysis of structures, which are used mainly in microelectronics and micromechanics, are demonstrated. Specific examples of the use of optical microscopy for qualitative and quantitative analysis, development of technological processes, control of defects, analysis of surface relief, determination of the parameters of structures are given. Surface measurements are performed in reflected and transmitted light, using bright and dark field modes.

scholarly journals Dark-Field Hyperspectral Microscopy for Carbon Nanotubes Bioimaging

2021 ◽  
Vol 11 (24) ◽  
pp. 12132
Author(s):  
Ilnur Ishmukhametov ◽  
Rawil Fakhrullin
Keyword(s):  
Carbon Nanotubes ◽  
Carbon Nanotube ◽  
Optical Microscopy ◽  
Biomedical Applications ◽  
Dark Field ◽  
Chemical Identification ◽  
Nanoscale Particles ◽  
Spectral Identification ◽  
Tyndall Effect ◽  
Dark Field Microscopy

Carbon nanotubes have emerged as a versatile and ubiquitous nanomaterial, finding applications in industry and biomedicine. As a result, biosafety concerns that stimulated the research focused on evaluation of carbon nanotube toxicity. In addition, biomedical applications of carbon nanotubes require their imaging and identification in biological specimens. Among other methods, dark-field microscopy has become a potent tool to visualise and identify carbon nanotubes in cells, tissues, and organisms. Based on the Tyndall effect, dark-field optical microscopy at higher magnification is capable of imaging nanoscale particles in live objects. If reinforced with spectral identification, this technology can be utilised for chemical identification and mapping of carbon nanotubes. In this article we overview the recent advances in dark-field/hyperspectral microscopy for the bioimaging of carbon nanotubes.

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