Comprehensive description of light scattering by simultaneous measurement of angular, spectral, and polarization dependence for characterization of tissue macroachitecture in normal and precancerous states

2003 ◽  
Author(s):  
Young L. Kim ◽  
Yang Liu ◽  
Ramesh K. Wali ◽  
Hemant J. Roy ◽  
Michael J. Goldberg ◽  
...  
Keyword(s):  
Light Scattering ◽  
Simultaneous Measurement ◽  
Polarization Dependence ◽  
Comprehensive Description

scholarly journals Characterization of gelification of chitosan solutions by dynamic light scattering

2012 ◽  
Vol 87 (4) ◽  
pp. 2376-2380 ◽  
Author(s):  
W.A. de Morais ◽  
M.R. Pereira ◽  
J.L.C. Fonseca
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering

scholarly journals Characterization of Recombinant Adeno-Associated Viruses (rAAVs) for Gene Therapy Using Orthogonal Techniques

Pharmaceutics ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 586
Author(s):  
Liam Cole ◽  
Diogo Fernandes ◽  
Maryam T. Hussain ◽  
Michael Kaszuba ◽  
John Stenson ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Viral Vector ◽  
Structural Characteristics ◽  
Genetic Material ◽  
Label Free ◽  
Gene Therapies ◽  
Multiple Challenges

Viruses are increasingly used as vectors for delivery of genetic material for gene therapy and vaccine applications. Recombinant adeno-associated viruses (rAAVs) are a class of viral vector that is being investigated intensively in the development of gene therapies. To develop efficient rAAV therapies produced through controlled and economical manufacturing processes, multiple challenges need to be addressed starting from viral capsid design through identification of optimal process and formulation conditions to comprehensive quality control. Addressing these challenges requires fit-for-purpose analytics for extensive characterization of rAAV samples including measurements of capsid or particle titer, percentage of full rAAV particles, particle size, aggregate formation, thermal stability, genome release, and capsid charge, all of which may impact critical quality attributes of the final product. Importantly, there is a need for rapid analytical solutions not relying on the use of dedicated reagents and costly reference standards. In this study, we evaluate the capabilities of dynamic light scattering, multiangle dynamic light scattering, and SEC–MALS for analyses of rAAV5 samples in a broad range of viral concentrations (titers) at different levels of genome loading, sample heterogeneity, and sample conditions. The study shows that DLS and MADLS® can be used to determine the size of full and empty rAAV5 (27 ± 0.3 and 33 ± 0.4 nm, respectively). A linear range for rAAV5 size and titer determination with MADLS was established to be 4.4 × 1011–8.7 × 1013 cp/mL for the nominally full rAAV5 samples and 3.4 × 1011–7 × 1013 cp/mL for the nominally empty rAAV5 samples with 3–8% and 10–37% CV for the full and empty rAAV5 samples, respectively. The structural stability and viral load release were also inferred from a combination of DLS, SEC–MALS, and DSC. The structural characteristics of the rAAV5 start to change from 40 °C onward, with increasing aggregation observed. With this study, we explored and demonstrated the applicability and value of orthogonal and complementary label-free technologies for enhanced serotype-independent characterization of key properties and stability profiles of rAAV5 samples.

Polarized light-scattering profile-advanced characterization of nonspherical particles with scanning flow cytometry

2011 ◽  
Vol 79A (7) ◽  
pp. 570-579 ◽  
Author(s):  
Dmitry I. Strokotov ◽  
Alexander E. Moskalensky ◽  
Vyacheslav M. Nekrasov ◽  
Valeri P. Maltsev
Keyword(s):  
Flow Cytometry ◽  
Light Scattering ◽  
Polarized Light ◽  
Advanced Characterization ◽  
Nonspherical Particles
Sign in / Sign up

Export Citation Format

Share Document