Laser photodynamic therapy of cancer: the chorioallantoic membrane model for measuring damage to blood vessels in-vivo

1991 ◽  
Author(s):  
Varda Gottfried ◽  
Ella S. Lindenbaum ◽  
Sol Kimel ◽  
Marie J. Hammer-Wilson ◽  
Michael W. Berns
Keyword(s):  
Photodynamic Therapy ◽  
Blood Vessels ◽  
Chorioallantoic Membrane ◽  
Membrane Model ◽  
Photodynamic Therapy Of Cancer

scholarly journals Circulation Index as a Quantitative Indicator of Angiogenesis in Chorioallantoic Membrane of Chicken Broilers

2018 ◽  
Vol 67 (2) ◽  
pp. 164-170
Author(s):  
Zoran Ružić ◽  
Zdenko Kanački ◽  
Dragan Žikić ◽  
Gordana Ušćebrka ◽  
Jovan Mirčeta
Keyword(s):  
Embryonic Development ◽  
Blood Vessels ◽  
Chorioallantoic Membrane ◽  
New Method ◽  
In Vivo Studies ◽  
Circulation Index ◽  
Fast Method ◽  
Angiogenic Response ◽  
Embryonic Life

Summary Chorioallantoic membrane (CAM) is an extraembryonic membrane very frequently used for in vivo studies in various researches. Since researches require a fast method for quantifying the CAM angiogenic response, there is a need to develop a new precise and unbiased method of quantification of angiogenesis in CAM, which would be easy to perform and suitable for analysis of a large number of samples. The objective of this paper is to apply a new method of quantification of angiogenesis in investigation of the development of blood vessels in the CAM, in particular days of embryonic life considered essential for CAM development. The present research included 75 fertilized eggs of heavy hybrid Ross 308. CAM sampling for stereological analyses was in key phases of embryonic development, namely on the 12th, 15th and 19th day. The results of the present investigation show that the increase in embryonic age results in increase in circulation index, which is also an indicator of angiogenic processes developing in CAM. The lowest value of circulation index (0.1952) was recorded on the first sampling day (E12), while the highest value (0.2666) was recorded on the last sampling day (E19). This method may be applied in researching different factors which affect angiogenesis in CAM.

scholarly journals Production of a heparin-binding angiogenesis factor by the embryonic kidney.

1986 ◽  
Vol 103 (3) ◽  
pp. 1101-1107 ◽  
Author(s):  
W Risau ◽  
P Ekblom
Keyword(s):  
Endothelial Cells ◽  
Growth Factor ◽  
Blood Vessels ◽  
Gel Filtration ◽  
Chorioallantoic Membrane ◽  
Heparin Binding ◽  
Rabbit Cornea ◽  
Embryonic Mouse ◽  
Angiogenesis Factor

Embryonic mouse kidneys induce angiogenesis when transplanted on the quail chorioallantoic membrane (Ekblom, P., H. Sariola, M. Karkinen, and L. Saxén, 1982, Cell Differ., 11:35-39). In these experiments all blood vessels were derived from the quail host, suggesting that kidney endothelium is derived from outside blood vessels. We have now analyzed whether kidney angiogenesis is regulated by kidney-derived soluble factors that stimulate the growth of new blood vessels. In the rabbit cornea, 11-d embryonic kidneys induced angiogenesis, whereas uninduced 11-d kidney mesenchymes did not. To characterize and purify this activity from an embryonic organ, we dissected between 600 and 1,000 14-17-d-old embryonic mouse kidneys for each purification experiment. Growth factor activity for capillary endothelial cells was found to bind to heparin-Sepharose and eluted at 0.9-1.1 M sodium chloride. Gel filtration revealed a molecular weight of 16,000-20,000 of this factor. A major 18,000-mol-wt band was seen after gel electrophoresis and silver staining of partially purified growth factor material. The chromatographed factor is mitogenic for endothelial cells but not for smooth muscle cells and stimulates angiogenesis in vivo in the rabbit cornea. Adult kidneys contained two heparin-binding endothelial cell growth factors. The differentiation-dependent production of an angiogenesis factor by the embryonic kidney suggests an important role of angiogenesis in organogenesis.

Sign in / Sign up

Export Citation Format

Share Document