A study for the influence of semen extenders IMV-buffer, HIA- 1 and AU on
sperm motion characteristics of Muscovy drake spermatozoa was carried out.
The semen from each male (n=6) was divided into three equal parts and diluted
in ratio 1:3 (semen:extender) with the IMV-buffer, HIA-1 and AU respectively,
and then was stored at temperature 0-4C? for 6 hours. Sperm motion parameters
- velocity of spermatozoa (rapid, medium, slow and statistic), VCL, VSL, VAP,
LIN, STR, WOB were measured using a Sperm Class Analizer (Micropticum,
Spain). Computer-assisted sperm motion analysis indicated that Muscovy
spermatozoa preserved a rapid and medium sperm velocity after 6 hours in
vitro storage in three examination extenders. The total VCL, VSL and VAP of
spermatozoa in the semen diluted with AU extender were 110.47?7.44 ?m/s,
29.42?2.02 ?m/s and 57.39?3.73 ?m/s, in the semen diluted with IMV-buffer
were 94.93?11.10 ?m/s, 27.57?2.45 ?m/s and 51.35?4.98 ?m/s, and in the semen
diluted with HIA-1 were 68.48?12.74 ?m/s, 20.08?4.18 ?m/s and 37.75?7.65
?m/s, as the differences were significant between AU and HIA-1 - (P<0.05).
About LIN, STR, WOB there were no significant differences for the influence
of the extenders.