Compact two-photon laser-scanning microscope made from minimally modified commercial components

2002 ◽  
Author(s):  
Vijay Iyer ◽  
Tycho Hoogland ◽  
Bradley E. Losavio ◽  
A. R. McQuiston ◽  
Peter Saggau
Keyword(s):  
Laser Scanning ◽  
Scanning Microscope ◽  
Laser Scanning Microscope ◽  
Two Photon

Nondegenerate two-photon absorption properties of a newly synthesized carbazole derivative

2017 ◽  
Vol 5 (2) ◽  
pp. 470-475 ◽  
Author(s):  
Shu Chen ◽  
Yong-Chao Zheng ◽  
Mei-Ling Zheng ◽  
Xian-Zi Dong ◽  
Feng Jin ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Photon Absorption ◽  
Scanning Microscope ◽  
Absorption Properties ◽  
Carbazole Derivative ◽  
Laser Scanning Microscope ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Potential Use

Nondegenerate two-photon absorption of a newly carbazole derivative is reported, demonstrating its potential use in a two-color two-photon laser scanning microscope.

A custom confocal and two-photon digital laser scanning microscope

2000 ◽  
Vol 278 (6) ◽  
pp. H2150-H2156 ◽  
Author(s):  
W. Gil Wier ◽  
C. William Balke ◽  
Jeffrey A. Michael ◽  
Joseph R. H. Mauban
Keyword(s):  
Laser Scanning ◽  
Photon Counting ◽  
Objective Lens ◽  
Scanning Microscope ◽  
Fluorescence Detector ◽  
Laser Scanning Microscope ◽  
Ti:Sapphire Laser ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

We describe a custom one-photon (confocal) and two-photon all-digital (photon counting) laser scanning microscope. The confocal component uses two avalanche photodiodes (APDs) as the fluorescence detector to achieve high sensitivity and to overcome the limited photon counting rate of a single APD (∼5 MHz). The confocal component is approximately nine times more efficient than our commercial confocal microscope (fluorophore fluo 4). Switching from one-photon to two-photon excitation mode (Ti:sapphire laser) is accomplished by moving a single mirror beneath the objective lens. The pulse from the Ti:sapphire laser is 109 fs in duration at the specimen plane, and average power is ∼5 mW. Two-photon excited fluorescence is detected by a fast photomultiplier tube. With a ×63 1.4 NA oil-immersion objective, the resolution of the confocal system is 0.25 μm laterally and 0.52 μm axially. For the two-photon system, the corresponding values are 0.28 and 0.82 μm. The system is advantageous when excitation intensity must be limited, when fluorescence is low, or when thick, scattering specimens are being studied (with two-photon excitation).

Second harmonic imaging of chloroplasts using the two-photon laser scanning microscope

Micron ◽  
2009 ◽  
Vol 40 (3) ◽  
pp. 378-385 ◽  
Author(s):  
Ali Hussain Reshak ◽  
Vassilios Sarafis ◽  
Rainer Heintzmann
Keyword(s):  
Laser Scanning ◽  
Second Harmonic ◽  
Harmonic Imaging ◽  
Scanning Microscope ◽  
Laser Scanning Microscope ◽  
Two Photon

scholarly journals Method to quantify accuracy of position feedback signals of a three-dimensional two-photon laser-scanning microscope

2015 ◽  
Vol 6 (10) ◽  
pp. 3678 ◽  
Author(s):  
Michael Kummer ◽  
Knut Kirmse ◽  
Otto W. Witte ◽  
Jens Haueisen ◽  
Knut Holthoff
Keyword(s):  
Laser Scanning ◽  
Three Dimensional ◽  
Scanning Microscope ◽  
Laser Scanning Microscope ◽  
Two Photon ◽  
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