Development and use of a novel automated Mueller matrix polarization imaging system for In-vivo imaging of lesions

2002 ◽  
Author(s):  
Jung-Rae Chung ◽  
Justin S. Baba ◽  
Aimee H. DeLaughter ◽  
Gerard L. Cote
Keyword(s):  
In Vivo Imaging ◽  
Imaging System ◽  
Mueller Matrix ◽  
Polarization Imaging

scholarly journals Nanotechnology: from In Vivo Imaging System to Controlled Drug Delivery

2017 ◽  
Vol 12 (1) ◽  
Author(s):  
Maria Mir ◽  
Saba Ishtiaq ◽  
Samreen Rabia ◽  
Maryam Khatoon ◽  
Ahmad Zeb ◽  
...  
Keyword(s):  
Drug Delivery ◽  
In Vivo Imaging ◽  
Imaging System ◽  
Controlled Drug Delivery ◽  
In Vivo Imaging System

scholarly journals Preclinical orthotopic xenograft model of renal pelvis cancer in which cancer growth could be traced by an in vivo imaging system

2018 ◽  
Vol 26 (1) ◽  
pp. 138-139 ◽  
Author(s):  
Teruki Shimizu ◽  
Masatsugu Miyashita ◽  
Atsuko Fujihara ◽  
Fumiya Hongo ◽  
Osamu Ukimura ◽  
...  
Keyword(s):  
Renal Pelvis ◽  
In Vivo Imaging ◽  
Imaging System ◽  
Xenograft Model ◽  
Cancer Growth ◽  
Orthotopic Xenograft ◽  
Orthotopic Xenograft Model ◽  
In Vivo Imaging System

116 INCORPORATION AND DEVELOPMENTAL TOXICITY OF QUANTUM DOT NANOPARTICLES IN AMPHIBIAN LARVAE

2017 ◽  
Vol 29 (1) ◽  
pp. 166 ◽  
Author(s):  
A. R. Julien ◽  
S. B. Park ◽  
C. K. Vance ◽  
P. L. Ryan ◽  
S. T. Willard ◽  
...  
Keyword(s):  
Quantum Dot ◽  
In Vivo Imaging ◽  
Half Life ◽  
Imaging System ◽  
Fluorescence Emission ◽  
Total Flux ◽  
Intestinal Lumen ◽  
Post Exposure ◽  
Amphibian Larvae

The use of nanoparticles both commercially and pharmaceutically has increased over the past decade, including fluorescent quantum dot nanoparticles (QD) in biochemical research for in vivo imaging. Previous studies have reported the toxic effects of nanoparticles, but their effects on larval metamorphosis and animal development and growth have not been thoroughly examined. Additionally, the method of uptake of nanoparticles by larval systems is unknown. Amphibian larvae are an ideal model for assessing toxicity because of their sensitivity to environmental contaminants and rapid and easily observable developmental stages. We used Anaxyrus fowleri tadpoles to investigate QD (≤ 25 nm diameter) integration into larvae and possible deleterious effects on their growth and development. Tadpoles (A. fowleri; n = 5/group) were placed in 24-well plates containing 1 mL of distilled water and increasing concentrations of QD (0, 1, and 2 nM) 72 h post-hatch. The fluorescence emission of QD in wells was detected at various time points (1, 2, 24, 48, and 72 h) using the in vivo imaging system (IVIS). A subset of tadpoles was killed (MS-222) and sectioned for histopathology. Remaining tadpoles were monitored throughout development. Fluorescence emission of QD in sectioned tadpoles was visualised using an EVOS Cell Imaging System. Developmental metrics of living tadpoles were recorded until metamorphosis. Fluorescence intensity between controls and dosage groups were analysed by ANOVA-1, followed by Student’s l.s.d. test to evaluate the effects of QD concentration and exposure time. The threshold of significance was P < 0.05. The rate of incorporation of QD into tadpoles was determined using the equation y = C + Ao*2(–x/t1/2), where t1/2 is the half-life of QD remaining in solution. The IVIS imaging revealed a rapid decrease of QD fluorescence (total flux) signals from the aqueous tadpole environment. Decreases in fluorescence occurred within 1 h post-exposure and appeared dose and time dependent, with signal nearly gone within 48 h. Half-life of total flux (time necessary for tadpoles to absorb half of the QD in solution) is 20.75 h (R2 = 0.92) and 2.54 h (R2 = 0.96) for 1 nm and 2 nm QD in solution, respectively. The EVOS imaging revealed integration of QD and localization into tadpole tissues. Fluorescence was exclusively found within the mouth, gills, and sections of the intestinal lumen of exposed tadpoles within the first hour. Dose-dependent increases in fluorescence within tissue were observed at each time-point. No signal was observed in controls. In remaining live tadpoles, QD treated tadpoles were smaller in size [t(34) = 2.35, P = 0.024] than controls. Findings reveal that (1) A. fowleri tadpoles integrate and accumulate nanoparticles, without detectable excretion within 72 h post-exposure, and (2) nanoparticles impede normal tadpole development. Ongoing studies are determining the effects of QD exposure on complete tadpole metamorphosis. The work was supported by USDA-ARS Biophotonics Initiative grant #58–6402–3-018.

NEMA NU 4-2008 and in vivo imaging performance of RAYCAN trans-PET/CT X5 small animal imaging system

2019 ◽  
Vol 64 (11) ◽  
pp. 115014 ◽  
Author(s):  
J Teuho ◽  
C Han ◽  
L Riehakainen ◽  
A Honkaniemi ◽  
M Tirri ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Imaging System ◽  
Small Animal Imaging ◽  
Small Animal ◽  
Animal Imaging ◽  
Pet Ct ◽  
Imaging Performance

Use of In Vivo Imaging System Technology in Leishmania major BALB/c Mouse Ear Infection Studies

2017 ◽  
Vol 55 (2) ◽  
pp. 429-435 ◽  
Author(s):  
Alicia Cawlfield ◽  
Brian Vesely ◽  
Franklyn Ngundam ◽  
Kirk Butler ◽  
Dylan Nugent ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Leishmania Major ◽  
Imaging System ◽  
System Technology ◽  
Ear Infection ◽  
Mouse Ear ◽  
In Vivo Imaging System

Development and calibration of an automated Mueller matrix polarization imaging system

2002 ◽  
Vol 7 (3) ◽  
pp. 341 ◽  
Author(s):  
Justin S. Baba ◽  
Jung-Rae Chung ◽  
Aimee H. DeLaughter ◽  
Brent D. Cameron ◽  
Gerard L. Coté
Keyword(s):  
Imaging System ◽  
Mueller Matrix ◽  
Polarization Imaging

Abstract P066: Imaging Autophagy in Living Mice

2011 ◽  
Vol 109 (suppl_1) ◽  
Author(s):  
Roberta A Gottlieb ◽  
M R Sayen ◽  
Chengqun Huang ◽  
Jennifer Ramil ◽  
Bruce Ito ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Heavy Chain ◽  
In Vivo Imaging ◽  
Imaging System ◽  
Life Sciences ◽  
In Vivo Imaging System ◽  
First Time ◽  
Homeostatic Response ◽  
Caliper Life

Autophagy is a homeostatic response to cellular stress. It has been shown to be potently upregulated in the heart in response to a variety of interventions. However, to date, it has not been possible to monitor autophagy without sacrificing the animal. Here we report the use of the Caliper Life Sciences Spectrum In Vivo Imaging System (IVIS) to image autophagy in homozygous transgenic mice expressing mCherryLC3 under control of the alpha myosin heavy chain promoter. Autophagy was stimulated by the administration of rapamycin (2mg/kg), and autophagosomal flux was blocked by administration of chloroquine (10mg/kg) ip. Mice were imaged at baseline and four hours later, using a protocol of 3 acquisitions of 15 seconds each. Total flux was 3.19±0.72 before drug administration and 3.93+1.10 after 4 hr, p<;0.01, n=14. These results show for the first time imaging of autophagy in hearts of live mice.

Quantitative analysis of cell tracing by in vivo imaging system

2010 ◽  
Vol 30 (4) ◽  
pp. 541-545 ◽  
Author(s):  
Junmeng Zheng ◽  
Lijun Xu ◽  
Hongmin Zhou ◽  
Weina Zhang ◽  
Zhonghua Chen
Keyword(s):  
Quantitative Analysis ◽  
In Vivo Imaging ◽  
Imaging System ◽  
Cell Tracing ◽  
In Vivo Imaging System
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