Measurement of cell surface protein dynamics by two-photon image correlation spectroscopy and image cross-correlation spectroscopy

2002 ◽  
Author(s):  
Paul W. Wiseman ◽  
Jeffrey A. Squier
Keyword(s):  
Cell Surface ◽  
Protein Dynamics ◽  
Cross Correlation ◽  
Surface Protein ◽  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Cell Surface Protein ◽  
Two Photon ◽  
Image Correlation Spectroscopy

Two-photon image correlation spectroscopy and image cross-correlation spectroscopy

2000 ◽  
Vol 200 (1) ◽  
pp. 14-25 ◽  
Author(s):  
P. W. Wiseman ◽  
J. A. Squier ◽  
M. H. Ellisman ◽  
K. R. Wilson
Keyword(s):  
Cross Correlation ◽  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Two Photon ◽  
Image Correlation Spectroscopy

Immunogold and immunoblot studies on a cell surface protein found in primary mesenchyme cells and in the cortical secretory vesicles of the sea urchin egg

1987 ◽  
Vol 45 ◽  
pp. 832-833
Author(s):  
G.L. Decker ◽  
M.C. Valdizan
Keyword(s):  
Cell Surface ◽  
Sea Urchin ◽  
Surface Protein ◽  
Egg Cell ◽  
Secretory Vesicles ◽  
Cell Surface Protein ◽  
Surface Complexes ◽  
Mesenchyme Cells ◽  
Lowicryl K4m ◽  
Primary Mesenchyme Cells

A monoclonal antibody designated MAb 1223 has been used to show that primary mesenchyme cells of the sea urchin embryo express a 130-kDa cell surface protein that may be directly involved in Ca2+ uptake required for growth of skeletal spicules. Other studies from this laboratory have shown that the 1223 antigen, although in relatively low abundance, is also expressed on the cell surfaces of unfertilized eggs and on the majority of blastomeres formed prior to differentiation of the primary mesenchyme cells.We have studied the distribution of 1223 antigen in S. purpuratus eggs and embryos and in isolated egg cell surface complexes that contain the cortical secretory vesicles. Specimens were fixed in 1.0% paraformaldehyde and 1.0% glutaraldehyde and embedded in Lowicryl K4M as previously reported. Colloidal gold (8nm diameter) was prepared by the method of Mulpfordt.

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