Fabrication of microstamps used for oligonucleotide array synthesis

2001 ◽  
Author(s):  
Pengfeng Xiao ◽  
Nongyao He ◽  
Zhengchun Liu ◽  
Quanguo He ◽  
Jiqing Xu ◽  
...  
Keyword(s):  
Oligonucleotide Array

scholarly journals Oligonucleotide array discovery of polymorphisms in cultivated tomato (Solanum lycopersicum L.) reveals patterns of SNP variation associated with breeding

BMC Genomics ◽  
2009 ◽  
Vol 10 (1) ◽  
pp. 466 ◽  
Author(s):  
Sung-Chur Sim ◽  
Matthew D Robbins ◽  
Charles Chilcott ◽  
Tong Zhu ◽  
David M Francis
Keyword(s):  
Solanum Lycopersicum ◽  
Oligonucleotide Array ◽  
Solanum Lycopersicum L

Abstract 347: Identification and Characterization of a miRNA Cohort Initiated Transitional Program That Controls Cell Cycle Arrest of the Perinatal Heart

2015 ◽  
Vol 117 (suppl_1) ◽  
Author(s):  
Patrick G Burgon ◽  
Jonathan J Weldrick
Keyword(s):  
Cell Cycle ◽  
Empirical Bayes ◽  
Molecular Mechanisms ◽  
Fetal Heart ◽  
Micro Rna ◽  
Bayes Estimation ◽  
Oligonucleotide Array ◽  
Array Analysis ◽  
Adult Heart ◽  
Perinatal Heart

During fetal and early perinatal development the myocardium undergoes a period of hyperplastic growth, which results in an exponential increase in the number of cardiomyocytes (CM) that will constitute the adult heart. Soon after birth, CMs proceed through a final round of cell division in the absence cytokinesis that results in binucleation of >95% of adult CMs. Fetal heart genes are re-activated with the onset of pathological hypertrophic or dilated cardiomyopathies, yet there is no evidence of CM re-entry into the cell cycle. Despite the importance of this phenomenon, little is known about the molecular basis for the transition from hyperplastic to hypertrophic-based myocardial growth. Hypothesis: A perinatal heart gene program is necessary for the normal transition from a fetal heart gene program to an adult heart gene program. To identify the molecular mechanisms and pathways involved in CM differentiation during the perinatal transition, RNA was isolated from E18, and 1, 3, 5, 7, 10 and 35d old mouse hearts. CM gene expression and micro-RNA profiles (n=3 arrays/time point) were determined by oligonucleotide array analysis. The raw array data was normalized by Robust Multi-array analysis. Empirical Bayes estimation of gene-specific variances was performed between each of the time points in order to identify genes that are transiently and significantly changed at days 3 and 5 as compare to E18 and 10d post-birth. The analysis identified 2,799 genes (E18 v 5d) and 3,347 genes (5d v 10d) that were then clustered to determine significant pathway enrichment (p<0.05) with Ingenuity Pathway Analysis. Our analysis confirmed previous observations of a down regulation of glucose oxidative metabolism (p=0.02) with an up-regulation of fatty acid metabolism (p=0.0001) between E18 and 5d post-birth. Also, 63 cell cycle genes are collectively down regulated (p=4.3x10-4) between 5d and 10d post-birth. We identified 131 genes that are transiently up regulated at 5d compared to E18 and 10d and this transition was proceeded by a specific cohort of miRNAs. The data generated from this study provide new insight into the molecular mechanisms by which CMs regulate and permanently exit from the cell cycle.

scholarly journals Application of custom-designed oligonucleotide array CGH in 145 patients with autistic spectrum disorders

2012 ◽  
Vol 21 (6) ◽  
pp. 620-625 ◽  
Author(s):  
Barbara Wiśniowiecka-Kowalnik ◽  
Monika Kastory-Bronowska ◽  
Magdalena Bartnik ◽  
Katarzyna Derwińska ◽  
Wanda Dymczak-Domini ◽  
...  
Keyword(s):  
Array Cgh ◽  
Oligonucleotide Array ◽  
Autistic Spectrum Disorders ◽  
Autistic Spectrum ◽  
Spectrum Disorders

Oligonucleotide Array Technologies for Gene Expression Profiling

2001 ◽  
Author(s):  
David Dorris ◽  
Ramesh Ramakrishnan ◽  
Tim Sendera ◽  
Scott Magnuson ◽  
Abhijit Mazumder
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Oligonucleotide Array

A novel 4p16.3 microduplication distal to WHSC1 and WHSC2 characterized by oligonucleotide array with new phenotypic features

2011 ◽  
Vol 155 (9) ◽  
pp. 2224-2228 ◽  
Author(s):  
Andrew B. Cyr ◽  
Manjunath Nimmakayalu ◽  
Susannah Q. Longmuir ◽  
Shivanand R. Patil ◽  
Kim M. Keppler-Noreuil ◽  
...  
Keyword(s):  
Oligonucleotide Array ◽  
Phenotypic Features
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