scholarly journals Time-resolved infrared studies of the dynamics of ligand binding to cytochrome c oxidase

1991 ◽  
Author(s):  
R. B. Dyer ◽  
Kristen A. Peterson ◽  
Page O. Stoutland ◽  
Oloef Einarsdottir ◽  
William H. Woodruff
Keyword(s):  
Cytochrome C ◽  
Ligand Binding ◽  
Cytochrome C Oxidase ◽  
Time Resolved ◽  
Infrared Studies

An Intermediate Conformational State during Ligand Binding to Cytochrome c Oxidase Detected by Time-resolved Resonance Raman Analyses of Heme Peripheral Groups

2012 ◽  
Vol 41 (2) ◽  
pp. 178-180 ◽  
Author(s):  
Izumi Ishigami ◽  
Takeshi Nishigaki ◽  
Kyoko Shinzawa-Itoh ◽  
Shinya Yoshikawa ◽  
Satoru Nakashima ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Ligand Binding ◽  
Cytochrome C Oxidase ◽  
Resonance Raman ◽  
Conformational State ◽  
Time Resolved

scholarly journals Probing the Proton-Loading Site of Cytochrome C Oxidase Using Time-Resolved Fourier Transform Infrared Spectroscopy

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3393
Author(s):  
Elena Gorbikova ◽  
Sergey A. Samsonov ◽  
Ruslan Kalendar
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Fourier Transform Infrared Spectroscopy ◽  
Paracoccus Denitrificans ◽  
Fourier Transform Infrared ◽  
Double Difference ◽  
Time Resolved ◽  
Fast Kinetic

Crystal structure analyses at atomic resolution and FTIR spectroscopic studies of cytochrome c oxidase have yet not revealed protonation or deprotonation of key sites of proton transfer in a time-resolved mode. Here, a sensitive technique to detect protolytic transitions is employed. In this work, probing a proton-loading site of cytochrome c oxidase from Paracoccus denitrificans with time-resolved Fourier transform infrared spectroscopy is presented for the first time. For this purpose, variants with single-site mutations of N131V, D124N, and E278Q, the key residues in the D-channel, were studied. The reaction of mutated CcO enzymes with oxygen was monitored and analyzed. Seven infrared bands in the “fast” kinetic spectra were found based on the following three requirements: (1) they are present in the “fast” phases of N131V and D124N mutants, (2) they have reciprocal counterparts in the “slow” kinetic spectra in these mutants, and (3) they are absent in “fast” kinetic spectra of the E278Q mutant. Moreover, the double-difference spectra between the first two mutants and E278Q revealed more IR bands that may belong to the proton-loading site protolytic transitions. From these results, it is assumed that several polar residues and/or water molecule cluster(s) share a proton as a proton-loading site. This site can be propionate itself (holding only a fraction of H+), His403, and/or water cluster(s).

scholarly journals Towards time-resolved structural studies of ba3-type cytochrome c oxidase

2018 ◽  
Vol 1859 ◽  
pp. e72
Author(s):  
Cecilia Safari ◽  
Swagatha Ghosh ◽  
Rebecka Andersson ◽  
Gisela Brändén
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Structural Studies ◽  
Time Resolved

Time-resolved FTIR Spectroscopy on Fully-Reduced CO Bound Cytochrome c Oxidase

2000 ◽  
Vol 28 (5) ◽  
pp. A189-A189
Author(s):  
D. Heitbrink ◽  
J. Heberle ◽  
C. Bolwien ◽  
H. Sigurdson ◽  
P. Brzesinski
Keyword(s):  
Cytochrome C ◽  
Ftir Spectroscopy ◽  
Cytochrome C Oxidase ◽  
Time Resolved

scholarly journals Time-Resolved Surface-Enhanced IR-Absorption Spectroscopy of Direct Electron Transfer to Cytochrome c Oxidase from R. sphaeroides

2013 ◽  
Vol 105 (12) ◽  
pp. 2706-2713 ◽  
Author(s):  
Andreas Schwaighofer ◽  
Christoph Steininger ◽  
David M. Hildenbrandt ◽  
Johannes Srajer ◽  
Christoph Nowak ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Absorption Spectroscopy ◽  
Direct Electron Transfer ◽  
Ir Absorption ◽  
Time Resolved ◽  
Surface Enhanced

scholarly journals A nanosecond time-resolved XFEL analysis of structural changes associated with CO release from cytochrome c oxidase

2017 ◽  
Vol 3 (7) ◽  
pp. e1603042 ◽  
Author(s):  
Atsuhiro Shimada ◽  
Minoru Kubo ◽  
Seiki Baba ◽  
Keitaro Yamashita ◽  
Kunio Hirata ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Structural Changes ◽  
Time Resolved
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