Characterization of fluorescence-labeled DNA by time-resolved fluorescence spectroscopy

1991 ◽  
Author(s):  
Claus A. M. Seidel ◽  
K. Rittinger ◽  
John A. Cortes ◽  
R. S. Goody ◽  
Malte Koellner ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Time Resolved Fluorescence ◽  
Time Resolved

Characterization of Plasma-Modified Fluoropolymer Surfaces Using Steady-State and Time-Resolved Fluorescence Spectroscopy

1994 ◽  
Vol 48 (5) ◽  
pp. 630-637 ◽  
Author(s):  
Ming Li ◽  
Michaeleen L. Pacholski ◽  
Frank V. Bright
Keyword(s):  
Steady State ◽  
Fluorescence Spectroscopy ◽  
Sulfonyl Chloride ◽  
Sodium Dodecylsulfate ◽  
Covalent Immobilization ◽  
Ambient Conditions ◽  
Time Resolved Fluorescence ◽  
Decay Kinetics ◽  
Time Resolved

Poly(hexafluoropropylene-co-tetrafluoroethylene) (FEP) has been widely used in biotechnology because of its unique surface properties and biocompatibility. Recent work from our group has shown that plasma discharge-modified FEP can be used as the substratum for development of a very stable immunosensor. This result has prompted us to study further this new surface under ambient conditions. In this paper, we report on the covalent immobilization of a pyrene residue (-Py) onto FEP-APS (FEP-aminopropyl silane) surfaces and the characterization of FEP-APS-Py using steady-state and time-resolved fluorescence spectroscopy. Among the immobilization schemes tested, we found that the covalent coupling of pyrene-sulfonyl chloride to FEP-APS is the easiest and yields the most photostable FEP-APS-Py derivative. Steady-state emission spectra of FEP-APS-Py in contact with H2O, β-cyclodextrin (β-CD), and sodium dodecylsulfate (SDS) aqueous solutions differ considerably from those of Py-SO3 in solution. Time-resolved fluorescence spectroscopy of FEP-APS-Py demonstrates that the decay kinetics are strongly affected by the presence of ionic quenchers and molecular oxygen, as well as β-CD and SDS. The results are consistent with the suggestion that the APS-Py moiety undergoes a slow time-dependent reconfiguration at the FEP/APS interface.

scholarly journals The study of polyplex formation and stability by time-resolved fluorescence spectroscopy of SYBR Green I-stained DNA

2014 ◽  
Vol 13 (12) ◽  
pp. 1680-1689 ◽  
Author(s):  
Cosimo D'Andrea ◽  
Daniele Pezzoli ◽  
Chiara Malloggi ◽  
Alessia Candeo ◽  
Giulio Capelli ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Sybr Green ◽  
Sybr Green I ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Reliable Tool ◽  
Dna Vector

Time-resolved fluorescence spectroscopy in combination with SYBR Green I-DNA dye is a reliable tool for the characterization of DNA/vector complexation state.

Characterization of the lipid and protein organization in HBsAg viral particles by steady-state and time-resolved fluorescence spectroscopy

Biochimie ◽  
2010 ◽  
Vol 92 (8) ◽  
pp. 994-1002 ◽  
Author(s):  
Vanille J. Greiner ◽  
Caroline Egelé ◽  
Sule Oncul ◽  
Frédéric Ronzon ◽  
Catherine Manin ◽  
...  
Keyword(s):  
Steady State ◽  
Fluorescence Spectroscopy ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Viral Particles
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