Discrimination between urinary tract tissue and urinary stones by fiber-optic-pulsed photothermal radiometry method in vivo

1991 ◽  
Author(s):  
Yuichiro Daidoh ◽  
Tsunenori Arai ◽  
Akira Suda ◽  
Makoto Kikuchi ◽  
Yukikuni Komine ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Fiber Optic ◽  
Photothermal Radiometry ◽  
Urinary Stones ◽  
Pulsed Photothermal Radiometry

scholarly journals Accurate measurement of blood vessel depth in port wine stained human skin in vivo using pulsed photothermal radiometry

2004 ◽  
Vol 9 (5) ◽  
pp. 961 ◽  
Author(s):  
Bincheng Li ◽  
Boris Majaron ◽  
John A. Viator ◽  
Thomas E. Milner ◽  
Zhongping Chen ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Human Skin ◽  
Photothermal Radiometry ◽  
Port Wine ◽  
Pulsed Photothermal Radiometry

Fiber Optic Pulsed Photothermal Radiometry For Urinary Stone Diagnosis

1989 ◽  
Author(s):  
Yuichiro Daidoh ◽  
Yukikuni Komine ◽  
Hiroshi Nakamura ◽  
Tsunenori Arai ◽  
Masato Nakagawa ◽  
...  
Keyword(s):  
Fiber Optic ◽  
Urinary Stone ◽  
Photothermal Radiometry ◽  
Pulsed Photothermal Radiometry

scholarly journals Influence of Urinary Stones on the Composition of a 24-Hour Urine Sample

2003 ◽  
Vol 49 (2) ◽  
pp. 281-285 ◽  
Author(s):  
Norbert Laube ◽  
Michael Pullmann ◽  
Stefan Hergarten ◽  
Albrecht Hesse
Keyword(s):  
Urinary Tract ◽  
Stone Formation ◽  
Urinary Stones ◽  
Urinary Concentration ◽  
Oxalate Concentration ◽  
Depletion Effect ◽  
Order Of Magnitude ◽  
Stone Growth

Abstract Background: It can be assumed that stones in the urinary tract continuously increase in size by incorporating material from urine. Consequently, urine will exhibit depleted concentrations of lithogenic constituents when urinary stones are present in the patient’s urinary tract. Methods: To calculate the influence of the depletion effect, we considered two different models of stone growth. In the first model, the increase in stone size depends only on the urinary concentration of a lithogenic substance; the second model also considers the surface area of the growing stone. The case of only one kidney being affected by stone formation is considered separately. We discuss example calculations involving the formation of calcium oxalate. Results: The calculated depletion effects are of a nonnegligible order of magnitude. Assuming both a measured oxalate concentration of, e.g., 0.37 mmol/L and a reasonable in vivo stone growing rate of 10 mm3/day, a relative underestimation of the real “in situ” oxalate concentration between ∼21% (model 1) and ∼42% (model 2) occurs. The depletion effect increases markedly with increasing stone growth rate. Conclusions: Metabolic status can be evaluated correctly only in patients who have been declared “stone-free”, e.g., after stone removal. Because the expected stone-related depletion effect in most cases is of high clinical relevance, we recommend estimating the effect of the order of magnitude of the depletion on actual urinary composition.

scholarly journals degS Is Necessary for Virulence and Is among Extraintestinal Escherichia coli Genes Induced in Murine Peritonitis

2003 ◽  
Vol 71 (6) ◽  
pp. 3088-3096 ◽  
Author(s):  
Peter Redford ◽  
Paula L. Roesch ◽  
Rodney A. Welch
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Luria Bertani ◽  
Broth Culture ◽  
Wild Type ◽  
E Coli ◽  
Virulence Attenuation

ABSTRACT Extraintestinal Escherichia coli strains cause meningitis, sepsis, urinary tract infection, and other infections outside the bowel. We examined here extraintestinal E. coli strain CFT073 by differential fluorescence induction. Pools of CFT073 clones carrying a CFT073 genomic fragment library in a promoterless gfp vector were inoculated intraperitoneally into mice; bacteria were recovered by lavage 6 h later and then subjected to fluorescence-activated cell sorting. Eleven promoters were found to be active in the mouse but not in Luria-Bertani (LB) broth culture. Three are linked to genes for enterobactin, aerobactin, and yersiniabactin. Three others are linked to the metabolic genes metA, gltB, and sucA, and another was linked to iha, a possible adhesin. Three lie before open reading frames of unknown function. One promoter is associated with degS, an inner membrane protease. Mutants of the in vivo-induced loci were tested in competition with the wild type in mouse peritonitis. Of the mutants tested, only CFT073 degS was found to be attenuated in peritoneal and in urinary tract infection, with virulence restored by complementation. CFT073 degS shows growth similar to that of the wild type at 37°C but is impaired at 43°C or in 3% ethanol LB broth at 37°C. Compared to the wild type, the mutant shows similar serum survival, motility, hemolysis, erythrocyte agglutination, and tolerance to oxidative stress. It also has the same lipopolysaccharide appearance on a silver-stained gel. The basis for the virulence attenuation is unclear, but because DegS is needed for σE activity, our findings implicate σE and its regulon in E. coli extraintestinal pathogenesis.

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