In-vitro ablation of fibrocartilage by XeCl excimer laser

1991 ◽  
Author(s):  
Martin Buchelt ◽  
Thanassis Papaioannou ◽  
Michael C. Fishbein ◽  
Werner Peters ◽  
Clain Beeder ◽  
...  
HortScience ◽  
1991 ◽  
Vol 26 (6) ◽  
pp. 757B-757 ◽  
Author(s):  
Karim H. Al-Juboory

Shoots of greenhouse-grown Pothos were surface disinfested and explanted on modified Murashige and Skoog (MS) medium. Later they were treated with pulsed XeCl excimer laser radiation for 30 sec. Cultures treated with 12 or 25 pulses of excimer laser radiation showed only 23% and 10% contamination, respectively, versus 75% control. Inaddition, we demonstrated that pulsed XeCl excimer laser radiation affected the subsequent growth and regenerability of in vitro plants. The reason for this increased growth needs further investigation. Both BA and TDZ were important for increasing the number of shoots generated from a microshoot as well as inducing shoot organogenesis from Pothos callus. Of the 50 rooted ex vitro plants from this experiment only 30% were variegated like parental clone. The others were either pure green or albino, suggesting chimeral segregation.


HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1150a-1150
Author(s):  
Karim H. Al-Juboory ◽  
David J. Williams ◽  
M.H. Nayfeh

This study was initiated to investigate the effect of pulsed XeCL excimer laser radiation (308 nm) at 25 and 50 pulses per second at radiation levels of 0, 100 mJ, 200 mJ, and 300 mJ on microorganism contamination of in vitro cultures of Heder a canariensis. Explants were cultured on Murashige and Skoog (MS) medium supplemented with 8 mg/BA and 0.2 mg/NAA. The medium was adjusted to pH 5.7. The cultures were maintained for 16-hours photoperiods at 25.5°C. One day following transfer the explants were treated with pulsed excimer laser radiation. The percentage of contamination was reduced by laser radiation treatments. The effect of 5 0 pulses of radiation was found to increase the petiole length, leaf blade width, and leaf number of explants.


2000 ◽  
Vol 28 (1) ◽  
pp. 24-28 ◽  
Author(s):  
Junichi SHIDA ◽  
Naoyuki KOBAYASHI ◽  
Hideaki KUSAMA

1991 ◽  
Vol 111 (4) ◽  
pp. 46-51 ◽  
Author(s):  
Hisato Okuda ◽  
Kazuhisa Katayama ◽  
Xiaojun Wang ◽  
Katsumi Masugata ◽  
Kiyoshi Yatsui

1998 ◽  
Vol 24 (12) ◽  
pp. 781-785 ◽  
Author(s):  
Matthias Folwaczny ◽  
Tim Liesenhoff ◽  
Norbert Lehn ◽  
Hans-Henning Horch

1994 ◽  
Vol 14 (1) ◽  
pp. 3-6 ◽  
Author(s):  
Andreas Baumbach ◽  
Karl K. Haase ◽  
Carsten Rose ◽  
Martin Oberhoff ◽  
Hartmut Hanke ◽  
...  

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