Orthogonal polarization spectral (OPS) imaging: a new technique for the visualization and study of microcirculation

2001 ◽  
Author(s):  
A. G. Harris ◽  
I. Sinitsina ◽  
S. Pahernik ◽  
S. Langer ◽  
E. von Dobschuetz ◽  
...  
Keyword(s):  
New Technique ◽  
Orthogonal Polarization ◽  
Orthogonal Polarization Spectral ◽  
Ops Imaging ◽  
A New Technique

Validation of OPS imaging for microvascular measurements during isovolumic hemodilution and low hematocrits

2002 ◽  
Vol 282 (4) ◽  
pp. H1502-H1509 ◽  
Author(s):  
A. G. Harris ◽  
I. Sinitsina ◽  
K. Messmer
Keyword(s):  
Capillary Density ◽  
Vessel Diameter ◽  
Orthogonal Polarization ◽  
Systematic Bias ◽  
Dorsal Skinfold Chamber ◽  
Reflected Light ◽  
Orthogonal Polarization Spectral ◽  
Wide Range ◽  
Ops Imaging ◽  
A New Technique

Orthogonal polarization spectral (OPS) imaging is a new technique that can be used to visualize the microcirculation with reflected light. It uses hemoglobin absorption to visualize the red blood cells (RBCs). Thus the method could fail at low hematocrit (Hct). The aim of this study was to validate OPS imaging for quantitative measurements of diameter and functional capillary density (FCD) under conditions of hemodilution of varying degrees to achieve a wide range of Hcts. The validation was performed in the dorsal skinfold chamber of nine awake Syrian golden hamsters. Measurements of vessel diameter and FCD were performed off-line using Cap-Image on video sequences captured using OPS imaging and standard intravital fluorescence microscopy at baseline, 85, 70, 55, and 40% of the initial Hct. For hemodilution, isovolumic exchange of blood for 6% Dextran 60 was performed. Bland-Altman plots for the vessel diameter and FCD show good agreement between the two methods for both parameters at all studied Hcts. As expected, there was a systematic bias of ∼4 μm in the diameter measurements since the RBC column was measured and not the intravascular diameter. In conclusion, OPS imaging can be used to measure diameter and FCD at a wide range of Hcts.

scholarly journals Comparison of OPS imaging and conventional capillary microscopy to study the human microcirculation

2001 ◽  
Vol 91 (1) ◽  
pp. 74-78 ◽  
Author(s):  
Keshen R. Mathura ◽  
Karlijn C. Vollebregt ◽  
Kees Boer ◽  
Jurgen C. De Graaff ◽  
Dirk T. Ubbink ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Venous Occlusion ◽  
Orthogonal Polarization ◽  
Capillary Microscopy ◽  
Cell Velocity ◽  
Orthogonal Polarization Spectral ◽  
Red Blood Cell Velocity ◽  
Ops Imaging

Orthogonal polarization spectral (OPS) imaging is a new clinical technique for observation of the microcirculation of organ surfaces. For validation purposes, we compared OPS images of the nailfold skin with those obtained from conventional capillary microscopy at rest and during venous occlusion in 10 male volunteers. These images were computer analyzed to provide red blood cell velocity and capillary diameters of the same nailfold capillaries at rest and during venous occlusion. Results showed that OPS images provided similar values for red blood cell velocity and capillary diameter as those obtained from capillary microscopy images. OPS imaging, however, provided significantly better image quality, as shown by comparison of image contrast between OPS imaging and capillary microscopy. This made image analysis better and easier to perform. It is anticipated, therefore, that OPS imaging will become a new and powerful technique in the study of the human microcirculation in vivo because it can be used on human internal organs.

Orthogonal Polarization Spectral Imaging in Microcirculation

2011 ◽  
Vol 216 ◽  
pp. 327-331
Author(s):  
Hong Wang ◽  
Jan Guo Cui ◽  
Yang Yang Liu ◽  
Guo Ming Chen ◽  
Sheng Ping Liu
Keyword(s):  
Quantitative Assessment ◽  
Near Infrared ◽  
Fluorescent Dyes ◽  
Wide Spectrum ◽  
Infrared Light ◽  
Orthogonal Polarization ◽  
Imaging Method ◽  
Great Effort ◽  
Orthogonal Polarization Spectral ◽  
Ops Imaging

The microcirculation plays a crucial role in the interaction between blood and tissues in physiological pathophysiological pharmacological and clinical states. Despite its significant role in numerous diseases including, hypertension diabetes, sepsis or multiple organ failure, visible and near-infrared light, offer a window into human and animal tissues due to reduced scattering and absorption. We review orthogonal polarization spectral (OPS) imaging method for direct visualization and quantitative assessment of human microcirculation at the bedside are limited. OPS imaging is a relatively new noninvasive method for assessment of human microcirculation without using fluorescent dyes. Recent clinical studies using OPS imaging in various pathological states have shown a wide spectrum of different clinical applications with evident impact on the diagnosis, treatment or prognosis assessment. Thus, there is a great effort to validate OPS imaging for various clinical purposes. The principles of OPS imaging, validation studies, its advantages, limitations, methods of quantitative assessment and current experience in clinical practice are also discussed.

Orthogonal Polarization Spectral (OPS) imaging demonstrates microvascular impairment in a porcine model of sepsis

CHEST Journal ◽  
2004 ◽  
Vol 126 (4) ◽  
pp. 864S
Author(s):  
Alexander J. Mathew ◽  
Felicitas Ross ◽  
Jasmeet Bajaj ◽  
S.B. Waheed ◽  
E. Kassas ◽  
...  
Keyword(s):  
Porcine Model ◽  
Orthogonal Polarization ◽  
Orthogonal Polarization Spectral ◽  
Ops Imaging ◽  
Microvascular Impairment

scholarly journals Orthogonal polarization spectral imaging

2007 ◽  
pp. 141-147
Author(s):  
V Černý ◽  
Z Turek ◽  
R Pařízková
Keyword(s):  
Quantitative Assessment ◽  
Fluorescent Dyes ◽  
Wide Spectrum ◽  
Critical Role ◽  
Multiple Organ ◽  
Orthogonal Polarization ◽  
Great Effort ◽  
Orthogonal Polarization Spectral ◽  
Ops Imaging ◽  
Current Experience

The microcirculation plays a crucial role in the interaction between blood and tissues both in physiological and pathophysiological states. Despite its critical role in numerous diseases including diabetes, hypertension, sepsis or multiple organ failure, methods for direct visualization and quantitative assessment of human microcirculation at the bedside are limited. Orthogonal polarization spectral (OPS) imaging is a relatively new noninvasive method for assessment of human microcirculation without using fluorescent dyes. Recent clinical studies using OPS imaging in various pathological states have shown a wide spectrum of different clinical applications with evident impact on the diagnosis, treatment or prognosis assessment. Thus, there is a great effort to validate OPS imaging for various clinical purposes. The principles of OPS imaging, validation studies, its advantages, limitations, methods of quantitative assessment and current experience in clinical practice are discussed.

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