Ex-vivo testing of crystalline lens substitutes: a pilot study

2000 ◽  
Author(s):  
Marie Hamaoui ◽  
Jean-Marie A. Parel ◽  
Fabrice Manns ◽  
Hassan Tahi ◽  
Arthur Ho ◽  
...  
Keyword(s):  
Pilot Study ◽  
Ex Vivo ◽  
Crystalline Lens ◽  
Ex Vivo Testing

scholarly journals Endoscopic Ex Vivo Evaluation of Bile Concentrations by Narrow Band Imaging: A Pilot Study

2015 ◽  
Vol 2015 ◽  
pp. 1-3
Author(s):  
Roberta Maselli ◽  
Haruhiro Inoue ◽  
Haruo Ikeda ◽  
Manabu Onimaru ◽  
Akira Yoshida ◽  
...  
Keyword(s):  
Pilot Study ◽  
White Light ◽  
Potential Role ◽  
Narrow Band ◽  
Ex Vivo ◽  
Narrow Band Imaging ◽  
Initial Study ◽  
Bile Concentration ◽  
Duodenogastroesophageal Reflux

Background. Bile juice plays a major role in duodenogastroesophageal reflux (DGERD). Several devices to directly measure the bile concentration have been proposed. We aimed to ex-vivo evaluate the bile concentration by narrow band imaging (NBI).Method. From six surgical cholecystectomies, the content of the gallbladders was aspirated and the total biliary acid (TBA) concentration was evaluated. 2 mL was employed for serial twofold dilutions. Each dilution was scoped. Images on white light (WL) and NBI were captured and grouped accordingly to NBI-appearance and TBA-concentration.Results. Nondiluted bile had a TBA-concentration of 61965 ± 32989 μmol/L. Final dilution (1 : 4096) had 1.16 μmol/L. NBI and correspondent WL images were grouped into seven groups, and an NBI/Bile scale was created.Conclusion. The scale showed that not only NBI scale but also white light scale could be useful to predict the bile concentration. This initial study shows that NBI has a potential role in the detection of DGERD and further investigation is warranted to distinguish the presence and the concentration of bile, especially at very low TBA concentrations.

About Puncture Testing Applied for Mechanical Characterization of Fetal Membranes

2014 ◽  
Vol 136 (11) ◽  
Author(s):  
Wilfried Bürzle ◽  
Edoardo Mazza ◽  
John J. Moore
Keyword(s):  
Deformation Behavior ◽  
Mechanical Characterization ◽  
Ex Vivo ◽  
Fetal Membrane ◽  
Membrane Rupture ◽  
Resistance To Deformation ◽  
Ex Vivo Testing ◽  
Insight Into

Puncture testing has been applied in several studies for the mechanical characterization of human fetal membrane (FM) tissue, and significant knowledge has been gained from these investigations. When comparing results of mechanical testing (puncture, inflation, and uniaxial tension), we have observed discrepancies in the rupture sequence of FM tissue and significant differences in the deformation behavior. This study was undertaken to clarify these discrepancies. Puncture experiments on FM samples were performed to reproduce previous findings, and numerical simulations were carried out to rationalize particular aspects of membrane failure. The results demonstrate that both rupture sequence and resistance to deformation depend on the samples' fixation. Soft fixation leads to slippage in the clamping, which reduces mechanical loading of the amnion layer and results in chorion rupturing first. Conversely, the stiffer, stronger, and less extensible amnion layer fails first if tight fixation is used. The results provide a novel insight into the interpretation of ex vivo testing as well as in vivo membrane rupture.

scholarly journals Lipofection of early passages of cell cultures derived from murine adenocarcinomas: In vitro and ex vivo testing of the thymidine kinase/ganciclovir system

2002 ◽  
Vol 9 (1) ◽  
pp. 96-99 ◽  
Author(s):  
Armando L Karara ◽  
Viviana F Bumaschny ◽  
Gabriel L Fiszman ◽  
Cecilia C Casais ◽  
Gerardo C Glikin ◽  
...  
Keyword(s):  
Thymidine Kinase ◽  
Cell Cultures ◽  
Ex Vivo ◽  
Ex Vivo Testing

scholarly journals Encapsulated cyclosporine does not change the composition of the human microbiota when assessed ex vivo and in vivo

2020 ◽  
Vol 69 (6) ◽  
pp. 854-863
Author(s):  
Catherine O'Reilly ◽  
Órla O’Sullivan ◽  
Paul D. Cotter ◽  
Paula M. O’Connor ◽  
Fergus Shanahan ◽  
...  
Keyword(s):  
Pilot Study ◽  
Gut Microbiota ◽  
Targeted Delivery ◽  
Healthy Subjects ◽  
Ex Vivo ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Microbiota Composition ◽  
Faecal Samples

Introduction. Management of steroid-refractory ulcerative colitis has predominantly involved treatment with systemic cyclosporine A (CyA) and infliximab. Aim. The purpose of this study was to assess the effect of using a colon-targeted delivery system CyA formulation on the composition and functionality of the gut microbiota. Methodology. Ex vivo faecal fermentations from six healthy control subjects were treated with coated minispheres (SmPill) with (+) or without (−) CyA and compared with a non-treated control in a model colon system. In addition, the in vivo effect of the SmPill+CyA formulation was investigated by analysing the gut microbiota in faecal samples collected before the administration of SmPill+CyA and after 7 consecutive days of administration from eight healthy subjects who participated in a pilot study. Results. Analysis of faecal samples by 16S rRNA gene sequencing indicated little variation in the diversity or relative abundance of the microbiota composition before or after treatment with SmPill minispheres with or without CyA ex vivo or with CyA in vivo. Short-chain fatty acid profiles were evaluated using gas chromatography, showing an increase in the concentration of n-butyrate (P=0.02) and acetate (P=0.32) in the faecal fermented samples incubated in the presence of SmPill minispheres with or without CyA. This indicated that increased acetate and butyrate production was attributed to a component of the coated minispheres rather than an effect of CyA on the microbiota. Butyrate and acetate levels also increased significantly (P=0.05 for both) in the faecal samples of healthy individuals following 7 days’ treatment with SmPill+CyA in the pilot study. Conclusion. SmPill minispheres with or without CyA at the clinically relevant doses tested here have negligible direct effects on the gut microbiota composition. Butyrate and acetate production increased, however, in the presence of the beads in an ex vivo model system as well as in vivo in healthy subjects. Importantly, this study also demonstrates the relevance and value of using ex vivo colon models to predict the in vivo impact of colon-targeted drugs directly on the gut microbiota.

scholarly journals Pilot study of pulmonary arterial branch sealing using energy devices in an ex vivo model

2014 ◽  
Vol 148 (6) ◽  
pp. 3219-3223 ◽  
Author(s):  
Moishe Liberman ◽  
Mohamed Khereba ◽  
Eric Goudie ◽  
Jordan Kazakov ◽  
Vicky Thiffault ◽  
...  
Keyword(s):  
Pilot Study ◽  
Ex Vivo ◽  
Ex Vivo Model ◽  
Energy Devices ◽  
Pulmonary Arterial ◽  
Arterial Branch

Pilot Study of the Correlation of Multiphoton Tomography of Ex Vivo Human Testis with Histology

2012 ◽  
Vol 188 (2) ◽  
pp. 538-543 ◽  
Author(s):  
Bobby B. Najari ◽  
Ranjith Ramasamy ◽  
Joshua Sterling ◽  
Amit Aggarwal ◽  
Seema Sheth ◽  
...  
Keyword(s):  
Pilot Study ◽  
Ex Vivo ◽  
Human Testis ◽  
Multiphoton Tomography

scholarly journals Design of a Multifunctional Forceps for Use in Endoscopic Surgery

2010 ◽  
Vol 4 (2) ◽  
Author(s):  
Andrew Rau ◽  
Mary Frecker ◽  
Abraham Mathew ◽  
Eric Pauli
Keyword(s):  
Minimally Invasive ◽  
Endoscopic Surgery ◽  
Minimally Invasive Surgical Procedures ◽  
Ex Vivo ◽  
Catastrophic Failure ◽  
Minimally Invasive Surgical ◽  
Design Characteristics ◽  
Fail Safe ◽  
Ex Vivo Testing ◽  
Good Agreement

This paper presents a 3.0 mm diameter endoscopic forceps design for use in minimally invasive surgical procedures, which require significant grasping and spreading forces. Models of the proposed design predict considerable improvements in the opening range (140%) and force application (87%) for both grasping and spreading when compared with currently used endoscopic forceps. Several of the tool’s design characteristics promote fail-safe malfunctions, including locking before catastrophic failure and the decreased likelihood in detached parts. Initial benchtop testing showed good agreement between prototype performance and model prediction. Frictional losses experienced during testing were found to depend on load orientation. A surgical prototype is currently being manufactured for ex vivo testing.

scholarly journals A Pilot Study of Wet Lung Using Lung Ultrasound Surface Wave Elastography in an Ex Vivo Swine Lung Model

2019 ◽  
Vol 9 (18) ◽  
pp. 3923 ◽  
Author(s):  
Xiaoming Zhang ◽  
Boran Zhou ◽  
Alex X. Zhang
Keyword(s):  
Surface Wave ◽  
Pilot Study ◽  
Wave Speed ◽  
Lung Ultrasound ◽  
Ex Vivo ◽  
Lung Model ◽  
Water Volume ◽  
Lung Water ◽  
Wave Speeds ◽  
Lung Sample

Extravascular lung water (EVLW) is a basic symptom of congestive heart failure and other conditions. Computed tomography (CT) is standard method used to assess EVLW, but it requires ionizing radiation and radiology facilities. Lung ultrasound reverberation artifacts called B-lines have been used to assess EVLW. However, analysis of B-line artifacts depends on expert interpretation and is subjective. Lung ultrasound surface wave elastography (LUSWE) was developed to measure lung surface wave speed. This pilot study aimed at measureing lung surface wave speed due to lung water in an ex vivo swine lung model. The surface wave speeds of a fresh ex vivo swine lung were measured at 100 Hz, 200 Hz, 300 Hz, and 400 Hz. An amount of water was then filled into the lung through its trachea. Ultrasound imaging was used to guide the water filling until significant changes were visible on the imaging. The lung surface wave speeds were measured again. It was found that the lung surface wave speed increases with frequency and decreases with water volume. These findings are confirmed by experimental results on an additional ex vivo swine lung sample.

scholarly journals Salivary lubricity (ex vivo) enhances upon moderate exercise: A pilot study

2020 ◽  
Vol 116 ◽  
pp. 104743
Author(s):  
Mark Hopkins ◽  
Christine Boesch ◽  
Matthew Lansdall ◽  
Conor Mullen ◽  
Alan Mighell ◽  
...  
Keyword(s):  
Pilot Study ◽  
Ex Vivo ◽  
Moderate Exercise

scholarly journals A pilot study of ex-vivo MRI-PDFF of donor livers for assessment of steatosis and predicting early graft dysfunction

PLoS ONE ◽  
2020 ◽  
Vol 15 (5) ◽  
pp. e0232006 ◽  
Author(s):  
Sanjaya K. Satapathy ◽  
Humberto C. Gonzalez ◽  
Jason Vanatta ◽  
Andrew Dyer ◽  
Wesley Angel ◽  
...  
Keyword(s):  
Pilot Study ◽  
Ex Vivo ◽  
Graft Dysfunction ◽  
Early Graft ◽  
Early Graft Dysfunction
Sign in / Sign up

Export Citation Format

Share Document