Two-photon excitation energy transfer microscopy

Author(s):  
Ammasi Periasamy
1997 ◽  
Vol 17 (3) ◽  
pp. 161-174 ◽  
Author(s):  
M. Nickoleit ◽  
A. Uhl ◽  
J. Bendig

The simultaneous two-photon excitation energy transfer (SEET) was demonstrated for the first time using trichromophoric model compounds. Two identical donors (A–antenna) were covalently linked to an energy acceptor unit (T–target) with different energy levels preventing energy transfer of a single photon. At high intensity illumination (laser exposure) of a trichromophoric system A∼T∼A (A–fluorescein, erythrosin; T-Estilbene), sufficient to excite both of the appended donor subunits, population of the target excited state may occur via simultaneous energy transfer of two photons, one from each donor. In order to restrict reverse energy transfer from the higher energy target to the lower energy donor(s) it is necessary that the excited target unit undergoes an efficient photoreaction. In the investigated case this was achieved by photoisomerization of the stilbene unit used for monitoring of the SEET.


2013 ◽  
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pp. 797-804 ◽  
Author(s):  
Daniel Collado ◽  
Patricia Remón ◽  
Yolanda Vida ◽  
Francisco Najera ◽  
Pratik Sen ◽  
...  

2013 ◽  
Vol 13 (2) ◽  
pp. 1368-1371 ◽  
Author(s):  
Limin An ◽  
Kefu Chao ◽  
Qinghui Zeng ◽  
Xueting Han ◽  
Ze Yuan ◽  
...  

2014 ◽  
Vol 20 (52) ◽  
pp. 17242-17242 ◽  
Author(s):  
Elizabeth Baggaley ◽  
Deng-Ke Cao ◽  
Daniel Sykes ◽  
Stanley W. Botchway ◽  
Julia A. Weinstein ◽  
...  

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