Detection of in-situ hybridization to human metaphase chromosomes by atomic force microscopy

2000 ◽  
Author(s):  
Naoaki Okamoto ◽  
Mitsuru Ishikawa
Keyword(s):  
Atomic Force Microscopy ◽  
In Situ Hybridization ◽  
Metaphase Chromosomes ◽  
Force Microscopy ◽  
Atomic Force

Analysis off cereal chromosomes by atomic force microscopy

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 439-444 ◽  
Author(s):  
Terence J. McMaster ◽  
Mervyn J. Miles ◽  
Mark O. Winfield ◽  
Angela Karp
Keyword(s):  
Atomic Force Microscopy ◽  
In Situ Hybridization ◽  
Optical Signal ◽  
3 Dimensional ◽  
Force Microscopy ◽  
C Banding ◽  
Plant Chromosomes ◽  
Atomic Force ◽  
High Resolution Images

Atomic force microscopy has been applied to the study of plant chromosomes from cereal grasses Triticum aestivum (bread wheat), Triticum tauschii, and Hordeum vulgare (barley). Using standard mitotic metaphase squashes, high resolution images have been obtained of untreated chromosomes and also of chromosomes after C-banding, N-banding, and in situ hybridization. The true 3-dimensional nature of the images permits detailed analysis of the surface structure and, on untreated uncoated chromosomes, surface features on a length scale consistent with nucleosome structures have been observed. C+ and N+ regions are manifest as areas of high relief on a slightly collapsed chromosome structure. In situ hybridization leads to a more severe degradation of the native structure, although it is still possible to correlate the optical signal with the topography of the hybridized chromosome. Key words : atomic force microscope, AFM, chromosomes, C-banding, in situ hybridization.

scholarly journals Imaging of RNA in situ hybridization by atomic force microscopy

1996 ◽  
Vol 182 (3) ◽  
pp. 192-199 ◽  
Author(s):  
W. H. J. KALLE ◽  
M. V. E. MACVILLE ◽  
M. P. C. VAN DE CORPUT ◽  
B. G. DE GROOTH ◽  
H. J. TANKE ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
In Situ Hybridization ◽  
Force Microscopy ◽  
Atomic Force ◽  
Rna In Situ Hybridization

Scanning Near-field Optical/Atomic Force Microscopy detection of fluorescence in situ hybridization signals beyond the optical limit

2003 ◽  
Vol 289 (2) ◽  
pp. 237-244 ◽  
Author(s):  
Daisuke Fukushi ◽  
Motoharu Shichiri ◽  
Shigeru Sugiyama ◽  
Tomoyuki Yoshino ◽  
Shoji Hagiwara ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Near Field ◽  
Force Microscopy ◽  
Atomic Force ◽  
Optical Limit

Atomic force microscopy of in situ deformed nickel thin films

1999 ◽  
Vol 353 (1-2) ◽  
pp. 194-200 ◽  
Author(s):  
C. Coupeau ◽  
J.F. Naud ◽  
F. Cleymand ◽  
P. Goudeau ◽  
J. Grilhé
Keyword(s):  
Thin Films ◽  
Atomic Force Microscopy ◽  
Force Microscopy ◽  
Nickel Thin Films ◽  
Atomic Force

Use of in-situ atomic force microscopy to monitor the biodegradation of polyhydroxyalkanoates (PHAs)

2001 ◽  
Vol 167 (1) ◽  
pp. 139-151 ◽  
Author(s):  
Connie J. Rossini ◽  
Justinn F. Arceo ◽  
Evan R. McCarney ◽  
Brian H. Augustine ◽  
Douglas E. Dennis ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Force Microscopy ◽  
Atomic Force

Surface morphology of As-deposited and laser-damaged dielectric mirror coatings studied in-situ by atomic force microscopy

1992 ◽  
Author(s):  
Mark R. Kozlowski ◽  
Michael C. Staggs ◽  
Mehdi Balooch ◽  
Robert J. Tench ◽  
Wigbert J. Siekhaus
Keyword(s):  
Atomic Force Microscopy ◽  
Surface Morphology ◽  
Force Microscopy ◽  
Dielectric Mirror ◽  
Atomic Force
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