Optimum Ho:YAG laser irradiation condition on percutaneous laser disk decompression (PLDD): in-vitro quantitive evaluation of influence on disk cells using three-dimensional cell culture system

1999 ◽  
Author(s):  
Masato Sato ◽  
Miya Ishihara ◽  
Tsunenori Arai ◽  
Takashi Asazuma ◽  
Toshiyuki Kikuchi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Laser Irradiation ◽  
Culture System ◽  
Three Dimensional ◽  
Irradiation Condition ◽  
Cell Culture System ◽  
Ho:Yag Laser ◽  
Laser Disk ◽  
Dimensional Cell

Ho:YAG laser: intervertebral disk cell interaction using three-dimensional cell culture system

2000 ◽  
Author(s):  
Masato Sato ◽  
Miya Ishihara ◽  
Tsunenori Arai ◽  
Takashi Asazuma ◽  
Toshiyuki Kikuchi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Three Dimensional ◽  
Cell Interaction ◽  
Intervertebral Disk ◽  
Cell Culture System ◽  
Ho:Yag Laser ◽  
Dimensional Cell

A novel in vitro permeability assay using three-dimensional cell culture system

2015 ◽  
Vol 205 ◽  
pp. 93-100 ◽  
Author(s):  
Jong Bong Lee ◽  
Sung Hwa Son ◽  
Min Chul Park ◽  
Tae Hwan Kim ◽  
Min Gi Kim ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Three Dimensional ◽  
Cell Culture System ◽  
Permeability Assay ◽  
Dimensional Cell

scholarly journals Evaluation of RGD functionalization in hybrid hydrogels as 3D neural stem cell culture systems

2018 ◽  
Vol 6 (3) ◽  
pp. 501-510 ◽  
Author(s):  
Emanuele Mauri ◽  
Alessandro Sacchetti ◽  
Nunzio Vicario ◽  
Luca Peruzzotti-Jametti ◽  
Filippo Rossi ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Cell Culture ◽  
Culture System ◽  
Three Dimensional ◽  
Cell Culture System ◽  
Nervous System Diseases ◽  
Central Nervous System Diseases ◽  
Hybrid Hydrogels ◽  
Cell Culture Systems ◽  
Dimensional Cell

The use of neural stem cells (NSCs) in cell therapy has become a powerful tool used for the treatment of central nervous system diseases and the design of a three-dimensional cell culture system to improve NSCs viability is a challenge.

scholarly journals Recent Advances in Three-Dimensional Stem Cell Culture Systems and Applications

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Xiaowen Wu ◽  
Junxiang Su ◽  
Jizhen Wei ◽  
Nan Jiang ◽  
Xuejun Ge
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Three Dimensional ◽  
Culture Method ◽  
Future Research ◽  
Two Dimensional ◽  
Culture Methods ◽  
Three Dimensional Culture ◽  
Dimensional Cell

Cell culture is one of the most core and fundamental techniques employed in the fields of biology and medicine. At present, although the two-dimensional cell culture method is commonly used in vitro, it is quite different from the cell growth microenvironment in vivo. In recent years, the limitations of two-dimensional culture and the advantages of three-dimensional culture have increasingly attracted more and more attentions. Compared to two-dimensional culture, three-dimensional culture system is better to realistically simulate the local microenvironment of cells, promote the exchange of information among cells and the extracellular matrix (ECM), and retain the original biological characteristics of stem cells. In this review, we first present three-dimensional cell culture methods from two aspects: a scaffold-free culture system and a scaffold-based culture system. The culture method and cell characterizations will be summarized. Then the application of three-dimensional cell culture system is further explored, such as in the fields of drug screening, organoids and assembloids. Finally, the directions for future research of three-dimensional cell culture are stated briefly.

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