Methods of achieving three-dimensional reconstruction of tissue at the ultrastructural level demonstrating the distribution of melanosomes within retinal pigment epithelium

1999 ◽  
Author(s):  
Cynthia A. Toth ◽  
Ewa Worniallo ◽  
Sarah F. Bailey ◽  
Benjamin A. Rockwell ◽  
Clarence P. Cain
Keyword(s):  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Three Dimensional ◽  
Ultrastructural Level ◽  
Three Dimensional Reconstruction ◽  
Dimensional Reconstruction

scholarly journals Three-dimensional shape analysis of peripapillary retinal pigment epithelium-basement membrane layer based on OCT radial images

2021 ◽  
Vol 10 (1) ◽  
pp. 453-464
Author(s):  
Junfei Tong ◽  
Pengfei Dong ◽  
Sachin Kedar ◽  
Deepta Ghate ◽  
Linxia Gu
Keyword(s):  
Mathematical Model ◽  
Retinal Pigment Epithelium ◽  
Basement Membrane ◽  
Optic Disc ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Three Dimensional ◽  
Full Potential ◽  
3D Morphology ◽  
Potential Index

Abstract The peripapillary retinal pigment epithelium-basement membrane (ppRPE/BM) layer angle was recently proposed as a potential index for estimating intracranial pressure noninvasively. However, the ppRPE/BM layer angle, measured from the optical coherence tomography (OCT) scans, varied across the radial directions of the optic disc. This made the ppRPE/BM layer angle difficult to be utilized in its full potential. In this study, we developed a mathematical model to quantify the ppRPE/BM layer angles across radial scans in relation to the ppRPE/BM 3D morphology in terms of its 3D angle and scanning tilt angles. Results showed that the variations of the ppRPE/BM layer angle across radial scans were well explained by its 3D angle and scanning tilt angles. The ppRPE/BM layer 3D angle was reversely fitted from the measured ppRPE/BM layer angles across radial directions with application to six eyes from four patients, who underwent medically necessary lumbar puncture. The fitted curve from our mathematical model matched well with the experimental measurements (R 2 > 0.9 in most cases). This further validated our mathematical model. The proposed model in this study has elucidated the variations of ppRPE/BM layer angle across 2D radial scans from the perspective of the ppRPE/BM layer 3D morphology. It is expected that the ppRPE/BM layer 3D angle developed in this study could be further exploited as a new biomarker for the optic disc.

Normal and regenerating rabbit retinal pigment epithelium studied by High voltage electron microscopy

1991 ◽  
Vol 49 ◽  
pp. 250-251
Author(s):  
G. E. Korte ◽  
M. J. Song
Keyword(s):  
Electron Microscopy ◽  
Retinal Pigment Epithelium ◽  
High Voltage ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Three Dimensional ◽  
Uranyl Acetate ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron ◽  
High Voltage Electron

During regeneration the retinal pigment epithelium (RPE) undergoes changes in cell shape and the distribution of organelles. To study these changes we used highvoltage electron microscopy (HVEM) to examine normal RPE cells and cells at different stages of maturation during regeneration induced by iv injections of sodium iodate, in rabbits. This chemical poisons much of the RPE, spared cells serving as a source of a new epithelial sheet. Tissue was processed for routine electron microscopy; serial sections 0.25 or 0.50 μm thick were mounted on Formvar coated slot grids, stained with uranyl acetate and lead citrate, and examined with an AEI 1.2 MV high-voltage electron microscope. Cytological details were observed in stereo pictures and in series of sections that were used to make three-dimensional reconstructions.

scholarly journals Three-Dimensional Characterization of Peripapillary Retinal Pigment Epithelium-Basement Membrane Layer in Patients following Lumbar Puncture

2020 ◽  
Vol 10 (5) ◽  
pp. 1559
Author(s):  
Junfei Tong ◽  
Pengfei Dong ◽  
Sachin Kedar ◽  
Deepta Ghate ◽  
Linxia Gu
Keyword(s):  
Optic Nerve ◽  
Retinal Pigment Epithelium ◽  
Linear Regression ◽  
Basement Membrane ◽  
Optic Nerve Head ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Three Dimensional ◽  
Automatic Method ◽  
Before And After

Purpose: To develop and test an innovative semi-automatic method for quantifying the three-dimensional morphology of the peripapillary retinal pigment epithelium-basement membrane (ppRPE/BM) layer, with application to lumbar puncture (LP) patients. Methods: Nineteen patients undergoing LP were recruited. The optic nerve head images of both eyes were acquired in 12 radial directions using optical coherence tomography (OCT) before and after LP. For each OCT image, the ppRPE/BM layer was automatically segmented with manual corrections by independent graders when necessary. The linear regression model of the ppRPE/BM layer was fitted using the least squares approach, and the ppRPE/BM layer angle was measured as the slope of the linear regression line. The Bland–Altman plots and intraclass correlations (ICC) were used to assess the inter-observer reliabilities in measuring the ppRPE/BM layer angle. The repeated measures ANOVA analysis was applied to determine whether the ppRPE/BM layer angle changes following LP varied across the radial directions. Results: The percentages of scans that required manual corrections were 24% and 32% in the right eyes and left eyes, respectively. The Bland–Altman plots and ICC demonstrated excellent inter-observer reliability. The ppRPE/BM layer angle varied significantly across the 12 radial OCT scanning directions both before and after LP. However, the LP-induced changes in the ppRPE/BM layer angle across different radial directions were not statistically significant (p-value > 0.01). Conclusions: The three-dimensional quantifications of the ppRPE/BM layer angles, enabled by the semi-automatic method, provided enhanced information of the optic nerve head structure. For LP patients, the ppRPE/BM layer angle changes following the LP did not vary significantly across various radial directions, indicating that it could be evaluated in any radial direction.

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