Cost-effective real-time multispectral digital video imaging

Author(s):  
David B. Duncan ◽  
Gregory Leeson
Author(s):  
Paul Oehlmann ◽  
Paul Osswald ◽  
Juan Camilo Blanco ◽  
Martin Friedrich ◽  
Dominik Rietzel ◽  
...  

AbstractWith industries pushing towards digitalized production, adaption to expectations and increasing requirements for modern applications, has brought additive manufacturing (AM) to the forefront of Industry 4.0. In fact, AM is a main accelerator for digital production with its possibilities in structural design, such as topology optimization, production flexibility, customization, product development, to name a few. Fused Filament Fabrication (FFF) is a widespread and practical tool for rapid prototyping that also demonstrates the importance of AM technologies through its accessibility to the general public by creating cost effective desktop solutions. An increasing integration of systems in an intelligent production environment also enables the generation of large-scale data to be used for process monitoring and process control. Deep learning as a form of artificial intelligence (AI) and more specifically, a method of machine learning (ML) is ideal for handling big data. This study uses a trained artificial neural network (ANN) model as a digital shadow to predict the force within the nozzle of an FFF printer using filament speed and nozzle temperatures as input data. After the ANN model was tested using data from a theoretical model it was implemented to predict the behavior using real-time printer data. For this purpose, an FFF printer was equipped with sensors that collect real time printer data during the printing process. The ANN model reflected the kinematics of melting and flow predicted by models currently available for various speeds of printing. The model allows for a deeper understanding of the influencing process parameters which ultimately results in the determination of the optimum combination of process speed and print quality.


Chemosensors ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 139
Author(s):  
Wiktoria Blaszczak ◽  
Zhengchu Tan ◽  
Pawel Swietach

A fundamental phenotype of cancer cells is their metabolic profile, which is routinely described in terms of glycolytic and respiratory rates. Various devices and protocols have been designed to quantify glycolysis and respiration from the rates of acid production and oxygen utilization, respectively, but many of these approaches have limitations, including concerns about their cost-ineffectiveness, inadequate normalization procedures, or short probing time-frames. As a result, many methods for measuring metabolism are incompatible with cell culture conditions, particularly in the context of high-throughput applications. Here, we present a simple plate-based approach for real-time measurements of acid production and oxygen depletion under typical culture conditions that enable metabolic monitoring for extended periods of time. Using this approach, it is possible to calculate metabolic fluxes and, uniquely, describe the system at steady-state. By controlling the conditions with respect to pH buffering, O2 diffusion, medium volume, and cell numbers, our workflow can accurately describe the metabolic phenotype of cells in terms of molar fluxes. This direct measure of glycolysis and respiration is conducive for between-runs and even between-laboratory comparisons. To illustrate the utility of this approach, we characterize the phenotype of pancreatic ductal adenocarcinoma cell lines and measure their response to a switch of metabolic substrate and the presence of metabolic inhibitors. In summary, the method can deliver a robust appraisal of metabolism in cell lines, with applications in drug screening and in quantitative studies of metabolic regulation.


2008 ◽  
Vol 3 (1) ◽  
pp. 106-115 ◽  
Author(s):  
Ting Zhang ◽  
Yuanxin Ouyang ◽  
Yang He

The RFID is not only a feasible, novel, and cost-effective candidate for daily object identification but it is also considered as a significant tool to provide traceable visibility along different stages of the aviation supply chain. In the air baggage handing application, the RFID tags are used to enhance the ability for baggage tracking, dispatching and conveyance so as to improve the management efficiency and the users’ satisfaction. We surveyed current related work and introduce the IATA RP1740c protocol used for the standard to recognize the baggage tags. One distributed aviation baggage traceable application is designed based on the RFID networks. We describe the RFID-based baggage tracking experiment in the BCIA (Beijing Capital International Airport). In this experiment the tags are sealed in the printed baggage label and the RFID readers are fixed in the certain interested positions of the BHS in the Terminal 2. We measure the accurate recognition rate and monitor the baggage’s real-time situation on the monitor’s screen. Through the analysis of the measured results within two months we emphasize the advantage of the adoption of RFID tags in this high noisy BHS environment. The economical benefits achieved by the extensive deployment of RFID in the baggage handing system are also outlined.


2000 ◽  
Vol 58 (2B) ◽  
pp. 424-427 ◽  
Author(s):  
PAULO R. M. DE BITTENCOURT ◽  
MARCOS C. SANDMANN ◽  
MARLUS S. MORO ◽  
JOÃO C. DE ARAÚJO

We revised 16 patients submitted to epilepsy surgery using a new method of digital, real-time, portable electrocorticography. Patients were operated upon over a period of 28 months. There were no complications. The exam was useful in 13 cases. The low installation and operational costs, the reliability and simplicity of the method, indicate it may be useful for defining the epileptogenic regions in a variety of circumnstances, including surgery for tumors, vascular malformations, and other cortical lesions associated with seizure disorders.


2017 ◽  
Vol 5 (2) ◽  
pp. 293-310 ◽  
Author(s):  
Ryan A. Kromer ◽  
Antonio Abellán ◽  
D. Jean Hutchinson ◽  
Matt Lato ◽  
Marie-Aurelie Chanut ◽  
...  

Abstract. We present an automated terrestrial laser scanning (ATLS) system with automatic near-real-time change detection processing. The ATLS system was tested on the Séchilienne landslide in France for a 6-week period with data collected at 30 min intervals. The purpose of developing the system was to fill the gap of high-temporal-resolution TLS monitoring studies of earth surface processes and to offer a cost-effective, light, portable alternative to ground-based interferometric synthetic aperture radar (GB-InSAR) deformation monitoring. During the study, we detected the flux of talus, displacement of the landslide and pre-failure deformation of discrete rockfall events. Additionally, we found the ATLS system to be an effective tool in monitoring landslide and rockfall processes despite missing points due to poor atmospheric conditions or rainfall. Furthermore, such a system has the potential to help us better understand a wide variety of slope processes at high levels of temporal detail.


Author(s):  
Robert Rice ◽  
Rand Decker ◽  
Newel Jensen ◽  
Ralph Patterson ◽  
Stanford Singer

The growth of winter travel on alpine roads in the western United States, a result of the demand for reliable winter access, has increased the hazard to motorists and highway maintenance personnel from snow avalanches. Configurations are presented for systems that can detect and provide, in real time, warnings to motorists and highway maintainers of roadway avalanches. These warnings include on-site traffic control signing, in-vehicle audio alarms for winter maintenance vehicles, and notifying maintenance facilities or centralized agency dispatchers. These avalanche detection and warning systems can detect an existing avalanche and use the avalanche’s remaining time of descent to initiate on-site alarms. Alternatively, real-time knowledge and notification of the onset of avalanching may be used to proactively manage the evolving hazard over an affected length or corridor of highway. These corridors can be several tens of kilometers in length and may be very remote, low-volume rural highways. As a consequence, these detection and warning systems must be cost-effective alternatives to existing avalanche hazard reduction technology. Results and experiences from the winters of 1997–1998 and 1998–1999 are presented, along with recommendations and criteria for future deployment of these automated natural hazard reduction systems for rural transportation corridors.


2014 ◽  
Vol 2014 ◽  
pp. 1-8
Author(s):  
Pricila da Silva Cunha ◽  
Heloisa B. Pena ◽  
Carla Sustek D’Angelo ◽  
Celia P. Koiffmann ◽  
Jill A. Rosenfeld ◽  
...  

Monosomy 1p36 is considered the most common subtelomeric deletion syndrome in humans and it accounts for 0.5–0.7% of all the cases of idiopathic intellectual disability. The molecular diagnosis is often made by microarray-based comparative genomic hybridization (aCGH), which has the drawback of being a high-cost technique. However, patients with classic monosomy 1p36 share some typical clinical characteristics that, together with its common prevalence, justify the development of a less expensive, targeted diagnostic method. In this study, we developed a simple, rapid, and inexpensive real-time quantitative PCR (qPCR) assay for targeted diagnosis of monosomy 1p36, easily accessible for low-budget laboratories in developing countries. For this, we have chosen two target genes which are deleted in the majority of patients with monosomy 1p36:PRKCZandSKI. In total, 39 patients previously diagnosed with monosomy 1p36 by aCGH, fluorescentin situhybridization (FISH), and/or multiplex ligation-dependent probe amplification (MLPA) all tested positive on our qPCR assay. By simultaneously using these two genes we have been able to detect 1p36 deletions with 100% sensitivity and 100% specificity. We conclude that qPCR ofPRKCZandSKIis a fast and accurate diagnostic test for monosomy 1p36, costing less than 10 US dollars in reagent costs.


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