Biosensor studies of collagen and laminin binding with immobilized Escherichia coli O157:H7 and inhibition with naturally occurring food additives

1999 ◽  
Author(s):  
Marjorie B. Medina
Keyword(s):  
Escherichia Coli ◽  
Food Additives ◽  
Escherichia Coli O157 ◽  
Naturally Occurring ◽  
Laminin Binding

Fate of Naturally Occurring Escherichia coli O157:H7 and Other Zoonotic Pathogens during Minimally Managed Bovine Feedlot Manure Composting Processes†

2013 ◽  
Vol 76 (8) ◽  
pp. 1308-1321 ◽  
Author(s):  
ELAINE D. BERRY ◽  
PATRICIA D. MILLNER ◽  
JAMES E. WELLS ◽  
NORASAK KALCHAYANAND ◽  
MICHAEL N. GUERINI
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
High Temperatures ◽  
Thermophilic Bacteria ◽  
Foodborne Illness ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Naturally Occurring ◽  
On Farm ◽  
Campylobacter Spp

Reducing Escherichia coli O157:H7 in livestock manures before application to cropland is critical for reducing the risk of foodborne illness associated with produce. Our objective was to determine the fate of naturally occurring E. coli O157:H7 and other pathogens during minimally managed on-farm bovine manure composting processes. Feedlot pen samples were screened to identify E. coli O157:H7–positive manure. Using this manure, four piles of each of three different composting formats were constructed in each of two replicate trials. Composting formats were (i) turned piles of manure plus hay and straw, (ii) static stockpiles of manure, and (iii) static piles of covered manure plus hay and straw. Temperatures in the tops, toes, and centers of the conical piles (ca. 6.0 m3 each) were monitored. Compost piles that were turned every 2 weeks achieved higher temperatures for longer periods in the tops and centers than did piles that were left static. E. coli O157:H7 was not recovered from top samples of turned piles of manure plus hay and straw at day 28 and beyond, but top samples from static piles were positive for the pathogen up to day 42 (static manure stockpiles) and day 56 (static covered piles of manure plus hay and straw). Salmonella, Campylobacter spp., and Listeria monocytogenes were not found in top or toe samples at the end of the composting period, but E. coli O157:H7 and Listeria spp. were recovered from toe samples at day 84. Our findings indicate that some minimally managed composting processes can reduce E. coli O157:H7 and other pathogens in bovine manure but may be affected by season and/or initial levels of indigenous thermophilic bacteria. Our results also highlight the importance of adequate C:N formulation of initial mixtures for the production of high temperatures and rapid composting, and the need for periodic turning of the piles to increase the likelihood that all parts of the mass are subjected to high temperatures.

Soil versus Pond Ash Surfacing of Feedlot Pens: Occurrence of Escherichia coli O157:H7 in Cattle and Persistence in Manure†

2010 ◽  
Vol 73 (7) ◽  
pp. 1269-1277 ◽  
Author(s):  
ELAINE D. BERRY ◽  
JAMES E. WELLS ◽  
TERRANCE M. ARTHUR ◽  
BRYAN L. WOODBURY ◽  
JOHN A. NIENABER ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sampling Period ◽  
Escherichia Coli O157 ◽  
Surface Material ◽  
Beef Heifers ◽  
Pond Ash ◽  
E Coli ◽  
Cattle Feces ◽  
Naturally Occurring ◽  
Survival Differences

Reducing Escherichia coli O157:H7 in cattle and their manure is critical for reducing the risk for human foodborne and waterborne illness. The objective of this study was to evaluate the effects of soil and pond ash surfaces for feedlot pens on the prevalence, levels, and/or persistence of naturally occurring E. coli O157:H7 and total E. coli in cattle (feces and hides) and manure. Cattle (128 beef heifers) were sorted among 16 pens: 8 surfaced with soil and 8 surfaced with pond ash. The prevalence of E. coli O157:H7 in feces decreased (P < 0.0001) during the study from 57.0% on day 0 to 3.9% on day 84 but did not differ (P ≥ 0.05) between cattle on soil and on pond ash pens at any sampling period. The prevalence of the pathogen on hides and in feedlot surface material (FSM) also decreased (P < 0.0001), with no effect of soil or pond ash surface (P ≥ 0.05). Similarly, levels of E. coli in FSM did not differ (P ≥ 0.05) at any sampling period, and there were no clear trends for survival differences of E. coli O157:H7 or E. coli in FSM between pond ash and soil surfaces, although E. coli populations survived at 5.0 log CFU/g of FSM on the pen surfaces 6 weeks after the cattle were removed. These results indicate that housing cattle on pens surfaced with pond ash versus pens surfaced with soil does not affect E. coli O157:H7 in cattle or their manure.

scholarly journals Deposition and disinfection of Escherichia coli O157:H7 on naturally occurring photoactive materials in a parallel plate chamber

2014 ◽  
Vol 16 (2) ◽  
pp. 194-202 ◽  
Author(s):  
Alicia A. Taylor ◽  
Indranil Chowdhury ◽  
Amy S. Gong ◽  
David M. Cwiertny ◽  
Sharon L. Walker
Keyword(s):  
Escherichia Coli ◽  
Parallel Plate ◽  
Escherichia Coli O157 ◽  
Naturally Occurring ◽  
Photoactive Materials

Soil Solarization Reduces Escherichia coli O157:H7 and Total Escherichia coli on Cattle Feedlot Pen Surfaces†

2012 ◽  
Vol 75 (1) ◽  
pp. 7-13 ◽  
Author(s):  
ELAINE D. BERRY ◽  
JAMES E. WELLS
Keyword(s):  
Escherichia Coli ◽  
Immunomagnetic Separation ◽  
Sampling Time ◽  
Soil Solarization ◽  
Food Crops ◽  
Escherichia Coli O157 ◽  
Cattle Production ◽  
E Coli ◽  
Log Reduction ◽  
Naturally Occurring

Feedlot pen soil is a source for transmission of Escherichia coli O157:H7, and therefore a target for preharvest strategies to reduce this pathogen in cattle. The objective of this study was to determine the ability of soil solarization to reduce E. coli O157:H7 in feedlot surface material (FSM). A feedlot pen was identified in which naturally occurring E. coli O157:H7 was prevalent and evenly distributed in the FSM. Forty plots 3 by 3 m were randomly assigned such that five plots of each of the solarization times of 0, 1, 2, 3, 4, 6, 8, and 10 weeks were examined. Temperature loggers were placed 7.5 cm below the surface of each plot, and plots to be solarized were covered with clear 6-mil polyethylene. At each sampling time, five FSM samples were collected from each of five solarized and five unsolarized plots. E. coli concentrations and E. coli O157:H7 presence by immunomagnetic separation and plating were determined for each FSM sample. Initial percentages of E. coli O157:H7–positive samples in control and solarized FSM were 84 and 80%, respectively, and did not differ (P > 0.05). E. coli O157:H7 was no longer detectable by 8 weeks of solarization, but was still detected in unsolarized FSM at 10 weeks. The average initial concentration of E. coli in FSM was 5.56 log CFU/g and did not differ between treatments (P > 0.05). There was a 2.0-log decrease of E. coli after 1 week of solarization, and a >3.0-log reduction of E. coli by week 6 of solarization (P < 0.05). E. coli levels remained unchanged in unsolarized FSM (P > 0.05). Daily peak FSM temperatures were on average 8.7°C higher for solarized FSM compared with unsolarized FSM, and reached temperatures as high as 57°C. Because soil solarization reduces E. coli O157:H7, this technique may be useful for reduction of persistence and transmission of this pathogen in cattle production, in addition to remediation of E. coli O157:H7–contaminated soil used to grow food crops.

Inactivation of Escherichia coli O157:H7 and Other Naturally Occurring Microorganisms in Apple Cider by Electron Beam Irradiation

2004 ◽  
Vol 67 (8) ◽  
pp. 1574-1577 ◽  
Author(s):  
HUI WANG ◽  
CHERYLL A. REITMEIER ◽  
BONITA A. GLATZ
Keyword(s):  
Escherichia Coli ◽  
Electron Beam ◽  
Electron Beam Irradiation ◽  
Escherichia Coli O157 ◽  
Beam Irradiation ◽  
Accelerator Facility ◽  
Apple Cider ◽  
E Coli ◽  
Naturally Occurring ◽  
D Values

Two Escherichia coli O157:H7 strains, SEA 13 B88 gfp 73ec and B6-914 gfp 90ec, together with two bacteria, three yeasts, and two molds that were randomly selected from a collection of microorganisms found on apples or in apple cider, were inoculated into apple cider and subjected to electron beam irradiation at several doses between 0.0 and 2.3 kGy at the Iowa State University Linear Accelerator Facility. The D-values for the E. coli O157:H7 strains ranged between 0.25 and 0.34 kGy; the D-values for most of the normal flora from apples ranged between 0.24 and 0.59 kGy. By taking into account possible variations in treatment conditions, it was calculated that irradiation at 2.47 kGy should achieve a 5-log reduction of E. coli O157:H7 in apple cider at the 95% confidence level. Naturally occurring yeasts might survive such irradiation treatment.

scholarly journals Transmission and Infectious Dose of Escherichia coli O157:H7 in Swine

2004 ◽  
Vol 70 (9) ◽  
pp. 5331-5335 ◽  
Author(s):  
N. A. Cornick ◽  
A. F. Helgerson
Keyword(s):  
Escherichia Coli ◽  
Gastrointestinal Tract ◽  
Management Practices ◽  
Reservoir Host ◽  
Escherichia Coli O157 ◽  
Infectious Dose ◽  
Fecal Shedding ◽  
E Coli ◽  
Naturally Occurring ◽  
Infected Donor

ABSTRACT Escherichia coli O157:H7 is only occasionally isolated from healthy swine, but some experimentally infected animals will shed the organism in their feces for at least 2 months. Potential explanations for the paucity of naturally occurring infections in swine, as compared to cattle, include a lack of animal-to-animal transmission so that the organism cannot be maintained within a herd, a high infectious dose, or herd management practices that prevent the maintenance of the organism in the gastrointestinal tract. We hypothesized that donor pigs infected with E. coli O157:H7 would transmit the organism to naïve pigs. We also determined the infectious dose and whether housing pigs individually on grated floors would decrease the magnitude or duration of fecal shedding. Infected donor pigs shedding <104 CFU of E. coli O157:H7 per g transmitted the organism to 6 of 12 naïve pigs exposed to them. The infectious dose of E. coli O157:H7 for 3-month-old pigs was approximately 6 × 103 CFU. There was no difference in the magnitude and duration of fecal shedding by pigs housed individually on grates compared to those housed two per pen on cement floors. These results suggest that swine do not have an innate resistance to colonization by E. coli O157:H7 and that they could serve as a reservoir host under suitable conditions.

Microbial Antagonists of Escherichia coli O157:H7 on Fresh-Cut Lettuce and Spinach

2009 ◽  
Vol 72 (7) ◽  
pp. 1569-1575 ◽  
Author(s):  
MICHAEL A. JOHNSTON ◽  
MARK A. HARRISON ◽  
RUTH A. MORROW
Keyword(s):  
Escherichia Coli ◽  
Inhibitory Activity ◽  
Antagonistic Activity ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Iceberg Lettuce ◽  
Naturally Occurring ◽  
Pathogen Load ◽  
Natural Microflora ◽  
Fresh Cut

Fresh-cut lettuce and spinach can become contaminated with pathogens at numerous points from the field to the retail market. Natural microflora present on fresh produce may help reduce the pathogen load. The objective of this study was to isolate natural microflora from fresh-cut iceberg lettuce and baby spinach and to determine whether these bacteria were antagonistic toward Escherichia coli O157:H7. Samples were collected under conditions that mimicked actual practices between production and retail sale. Evidence of naturally occurring microorganisms on fresh lettuce (295 isolates) and spinach (200 isolates) and of possible antagonistic activity toward E. coli O157:H7 was documented. Inhibitory activity by several isolates was due to either acid production or antimicrobial peptides. Bacteria with inhibitory activity were isolated from every step in the processing and handling of the fresh-cut iceberg lettuce and baby spinach.

scholarly journals Inhibitory Effect of Sodium Citrate, Sodium Nitrite and Cinnamaldehyde on Biofilm-forming Escherichia coli O157:H7

2021 ◽  
Vol 4 (2) ◽  
pp. 11-16
Author(s):  
A.H. Jauro ◽  
I. Shu’aibu ◽  
G. Lawan ◽  
M.T. Adamu ◽  
M.Y. Iliyasu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sodium Nitrite ◽  
Food Processing ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Sodium Citrate ◽  
Food Additives ◽  
Inhibitory Effect ◽  
Latex Agglutination ◽  
Escherichia Coli O157

The development of biofilms by the foodborne pathogens attached to surfaces in the food processing environments results in the deterioration of products, persistence of pathogenic bacteria and transmission of food-associated diseases. In addition, biofilms are more resistant to antimicrobials than their planktonic counterparts which make their elimination from food and the food processing facilities a great challenge. This study aim was to determine the inhibitory effect of food additives on biofilm forming Escherichia coli O157:H7. The isolate obtained was subjected to Gram’s staining and various biochemical identifications and later confirmed by latex agglutination test. Biofilm formation potential was done on Congo red media and the confirmed biofilm former was subjected to biofilm formation at 10℃ and 37℃ for 168hrs. Antimicrobial susceptibility testing, MIC, MBC, and antibiofilm effect was determined following CLSI 2017 guideline. The highest zone of growth inhibition of 31 mm was exhibited by cinnamaldehyde, sodium nitrite with 26 mm and sodium citrate with 13 mm. The MIC 2.5 mg/mL was recorded for sodium citrate, 0.25 mg/mL for sodium nitrite and 0.125 μl/mL for cinnamaldehyde. Strong biofilm was formed at 37 ℃ with 7.82 x 109 CFU/mL viable cells at 168hrs while 6.79 x 109 CFU/mL were obtained at 10 ℃. All the three additives showed antibiofilm effect (at 10℃ and 37℃), cinnamaldehyde exhibited 70%-90.1%, sodium nitrite; 70%-88.2% inhibition and sodium nitrite; 75%-88% inhibition respectively. This study showed that sodium citrate, sodium nitrite and cinnamaldehyde exerted strong antimicrobial and antibiofilm properties indicating their potential as good preservatives.

Inactivation of Escherichia coli O157:H7 on Cattle Hides by Caprylic Acid and β-Resorcylic Acid

2013 ◽  
Vol 76 (2) ◽  
pp. 318-322 ◽  
Author(s):  
SANGEETHA ANANDA BASKARAN ◽  
VARUNKUMAR BHATTARAM ◽  
INDU UPADHYAYA ◽  
ABHINAV UPADHYAY ◽  
ANUP KOLLANOOR-JOHNY ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
Caprylic Acid ◽  
Escherichia Coli O157 ◽  
Antimicrobial Effects ◽  
E Coli ◽  
Naturally Occurring ◽  
Presence And Absence ◽  
Bovine Feces

Two naturally occurring, generally recognized as safe compounds, namely, caprylic acid (CA) (1%) and β-resorcylic acid (BR) (1%), and their combination, applied at 23 and 60°C were evaluated for their antimicrobial effects against Escherichia coli O157:H7 on cattle hides in the presence and absence of bovine feces. Fresh cleaned cattle hides were cut into pieces (5 cm2), air dried, and inoculated with a five-strain mixture of nalidixic acid–resistant (50 μg/ml) E. coli O157:H7 (~8.0 log CFU). The hide samples were air dried under a biosafety hood for 2 h and sprayed with 95% ethanol, 1% CA, 1% BR, or a mixture of 1% CA and 1% BR at 23 or 60°C. The hide samples were kept at 23°C, and E. coli O157:H7 populations were determined at 2 and 5 min after treatment. Both CA and BR were effective in decreasing E. coli O157:H7 populations on hides by 3 to 4 log CFU/cm2 (P &lt; 0.05). Sterile bovine feces had no effect on the decontaminating property of CA and BR on cattle hides (P &gt; 0.05). Results of this study indicate that CA and BR could potentially be used to decontaminate cattle hides, but follow-up research under slaughterhouse conditions is warranted.

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