Multiphoton excitation fluorescence imaging applied to the study of embryo development

1998 ◽  
Author(s):  
David L. Wokosin ◽  
John G. White
Keyword(s):  
Fluorescence Imaging ◽  
Embryo Development ◽  
Multiphoton Excitation

All-solid-state ultrafast lasers facilitate multiphoton excitation fluorescence imaging

1996 ◽  
Vol 2 (4) ◽  
pp. 1051-1065 ◽  
Author(s):  
D.L. Wokosin ◽  
V. Centonze ◽  
J.G. White ◽  
D. Armstrong ◽  
G. Robertson ◽  
...  
Keyword(s):  
Solid State ◽  
Fluorescence Imaging ◽  
Ultrafast Lasers ◽  
Multiphoton Excitation

Fluorescence Imaging Techniques for Studying Drosophila Embryo Development

2008 ◽  
Vol 39 (1) ◽  
Author(s):  
Manos Mavrakis ◽  
Richa Rikhy ◽  
Mary Lilly ◽  
Jennifer Lippincott‐Schwartz
Keyword(s):  
Fluorescence Imaging ◽  
Embryo Development ◽  
Imaging Techniques ◽  
Drosophila Embryo

The influence of confocal microscope design on imaging performance

1993 ◽  
Vol 51 ◽  
pp. 144-145
Author(s):  
C J R Sheppard
Keyword(s):  
Image Quality ◽  
Fluorescence Imaging ◽  
Confocal Microscope ◽  
Industrial Applications ◽  
Three Dimensions ◽  
Design Parameters ◽  
Weak Signals ◽  
Size And Shape ◽  
Imaging Performance ◽  
Fundamental Limitation

The confocal microscope is now widely used in both biomedical and industrial applications for imaging, in three dimensions, objects with appreciable depth. There are now a range of different microscopes on the market, which have adopted a variety of different designs. The aim of this paper is to explore the effects on imaging performance of design parameters including the method of scanning, the type of detector, and the size and shape of the confocal aperture.It is becoming apparent that there is no such thing as an ideal confocal microscope: all systems have limitations and the best compromise depends on what the microscope is used for and how it is used. The most important compromise at present is between image quality and speed of scanning, which is particularly apparent when imaging with very weak signals. If great speed is not of importance, then the fundamental limitation for fluorescence imaging is the detection of sufficient numbers of photons before the fluorochrome bleaches.

Ubiquinone-BODIPY nanoparticles for tumor redox-responsive fluorescence imaging and photodynamic activity

2021 ◽  
Author(s):  
Byunghee Hwang ◽  
Tae-Il Kim ◽  
Hyunjin Kim ◽  
Sungjin Jeon ◽  
Yongdoo Choi ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Selective Activation ◽  
Turn On ◽  
Intracellular Environment ◽  
Redox Responsive ◽  
Photodynamic Activity

A ubiquinone-BODIPY photosensitizer self-assembles into nanoparticles (PS-Q-NPs) and undergoes selective activation within the highly reductive intracellular environment of tumors, resulting in “turn-on” fluorescence and photosensitizing activities.

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