Monte-Carlo simulation of retinal vessel profiles for the interpretation of in-vivo oxymetric measurements by imaging fundus reflectometry

1997 ◽  
Author(s):  
Martin Hammer ◽  
S. Leistritz ◽  
Lutz Leistritz ◽  
Dietrich Schweitzer ◽  
Eike Thamm ◽  
...  
Keyword(s):  
Monte Carlo Simulation ◽  
Monte Carlo ◽  
Retinal Vessel ◽  
Fundus Reflectometry

Study of the Influence of Radionuclide Biokinetics on the Efficiency of In Vivo Counting Using Monte Carlo Simulation

2009 ◽  
Vol 96 (5) ◽  
pp. 558-567 ◽  
Author(s):  
Stephanie Lamart ◽  
Eric Blanchardon ◽  
Andrey Molokanov ◽  
Gary H. Kramer ◽  
David Broggio ◽  
...  
Keyword(s):  
Monte Carlo Simulation ◽  
Monte Carlo

Monte Carlo simulation of near infrared autofluorescence measurements of in vivo skin

2011 ◽  
Vol 105 (3) ◽  
pp. 183-189 ◽  
Author(s):  
Shuang Wang ◽  
Jianhua Zhao ◽  
Harvey Lui ◽  
Qingli He ◽  
Haishan Zeng
Keyword(s):  
Monte Carlo Simulation ◽  
Monte Carlo ◽  
Near Infrared

scholarly journals Replication dynamics of recombination-dependent replication forks

2020 ◽  
Author(s):  
Karel Naiman ◽  
Eduard Campillo-Funollet ◽  
Adam T. Watson ◽  
Alice Budden ◽  
Izumi Miyabe ◽  
...  
Keyword(s):  
Monte Carlo Simulation ◽  
Monte Carlo ◽  
Homologous Recombination ◽  
Replication Fork ◽  
S Phase ◽  
Replication Forks ◽  
Leading Strand ◽  
The Stability ◽  
Lagging Strand

AbstractDNA replication fidelity is essential for maintaining genetic stability. Forks arrested at replication fork barriers can be stabilised by the intra-S phase checkpoint, subsequently being rescued by a converging fork, or resuming when the barrier is removed. However, some arrested forks cannot be stabilised and fork convergence cannot rescue in all situations. Thus, cells have developed homologous recombination-dependent mechanisms to restart persistently inactive forks. To understand HR-restart we use polymerase usage sequencing to visualize in vivo replication dynamics at an S. pombe replication barrier, RTS1, and model replication by Monte Carlo simulation. We show that HR-restarted forks synthesise both strands with Pol δ for up to 30 kb without maturing to a δ/ε configuration and that Pol α is not used significantly on either strand, suggesting the lagging strand template remains as a gap that is filled in by Pol δ later. We further demonstrate that HR-restarted forks progress uninterrupted through a fork barrier that arrests canonical forks. Finally, by manipulating lagging strand resection during HR-restart by deleting pku70, we show that the leading strand initiates replication at the same position, signifying the stability of the 3’ single strand in the context of increased resection.

scholarly journals Replication dynamics of recombination-dependent replication forks

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Karel Naiman ◽  
Eduard Campillo-Funollet ◽  
Adam T. Watson ◽  
Alice Budden ◽  
Izumi Miyabe ◽  
...  
Keyword(s):  
Monte Carlo Simulation ◽  
Monte Carlo ◽  
Homologous Recombination ◽  
Single Strand ◽  
Replication Forks ◽  
Leading Strand ◽  
The Stability ◽  
Lagging Strand

AbstractReplication forks restarted by homologous recombination are error prone and replicate both strands semi-conservatively using Pol δ. Here, we use polymerase usage sequencing to visualize in vivo replication dynamics of HR-restarted forks at an S. pombe replication barrier, RTS1, and model replication by Monte Carlo simulation. We show that HR-restarted forks synthesise both strands with Pol δ for up to 30 kb without maturing to a δ/ε configuration and that Pol α is not used significantly on either strand, suggesting the lagging strand template remains as a gap that is filled in by Pol δ later. We further demonstrate that HR-restarted forks progress uninterrupted through a fork barrier that arrests canonical forks. Finally, by manipulating lagging strand resection during HR-restart by deleting pku70, we show that the leading strand initiates replication at the same position, signifying the stability of the 3′ single strand in the context of increased resection.

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