Rapid observation of unfixed, unstained human skin biopsy specimens with confocal microscopy and visualization

1997 ◽  
Vol 2 (4) ◽  
pp. 437 ◽  
Author(s):  
Barry R. Masters
Keyword(s):  
Confocal Microscopy ◽  
Skin Biopsy ◽  
Human Skin ◽  
Biopsy Specimens ◽  
Human Skin Biopsy

scholarly journals 677 Human skin biopsy culture model maintains psoriasis disease features and demonstrates Pathway Engagement by Dexamethasone

2017 ◽  
Vol 137 (5) ◽  
pp. S116
Author(s):  
S. Huang ◽  
S. Grosskurth ◽  
L. Miller ◽  
H. Patel ◽  
L. Olson ◽  
...  
Keyword(s):  
Skin Biopsy ◽  
Human Skin ◽  
Culture Model ◽  
Human Skin Biopsy

scholarly journals Glomerular Immunodeposits of Patients with IgA Nephropathy Are Enriched for IgG Autoantibodies Specific for Galactose-Deficient IgA1

2019 ◽  
Vol 30 (10) ◽  
pp. 2017-2026 ◽  
Author(s):  
Dana V. Rizk ◽  
Manish K. Saha ◽  
Stacy Hall ◽  
Lea Novak ◽  
Rhubell Brown ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Iga Nephropathy ◽  
Kidney Biopsy ◽  
Immunofluorescence Microscopy ◽  
Kidney Tissue ◽  
Antigenic Specificity ◽  
Biopsy Specimens ◽  
Phospholipase A2 Receptor ◽  
Sds Page ◽  
Specific Igg

BackgroundIgA nephropathy (IgAN) is the leading primary GN worldwide. The disease is thought to result from glomerular deposition of circulating immune complexes of IgG bound to galactose-deficient IgA1 (Gd-IgA1). However, routine immunofluorescence microscopy fails to detect IgG in many kidney biopsies from patients with IgAN and the specificity of IgG in immunodeposits has not been tested.MethodsWe used remnant frozen kidney-biopsy specimens from 34 patients with IgAN; 14 were IgG-positive and 20 were IgG-negative by routine immunofluorescence microscopy. Six patients with primary membranous nephropathy (MN) and eight with lupus nephritis (LN) served as controls. IgG in the kidney tissue was extracted and its amount determined by ELISA. IgG molecular integrity was assessed by SDS-PAGE immunoblotting. Antigenic specificity of extracted IgG was determined by ELISA using phospholipase A2 receptor (PLA2R) or Gd-IgA1 as antigen. In addition, ten other IgAN cases, six IgG-positive and four IgG-negative by routine immunofluorescence, were used for colocalization studies by confocal microscopy.ResultsIgG extracted from MN but not IgAN immunodeposits reacted with PLA2R. Conversely, IgG extracted from IgAN but not MN or LN immunodeposits reacted with Gd-IgA1. Even IgAN kidney-biopsy specimens without IgG by routine immunofluorescence microscopy had IgG specific for Gd-IgA1. Confocal microscopy confirmed the presence of IgG in the IgAN biopsies with colocalization of glomerular IgA and IgG.ConclusionsThese results reveal for the first time that IgAN kidney biopsies, with or without IgG by routine immunofluorescence, contain Gd-IgA1–specific IgG autoantibodies. These findings support the importance of these autoantibodies in the pathogenesis of IgAN.

scholarly journals Inability of Polymerase Chain Reaction, Pyrosequencing, and Culture of Infected and Uninfected Site Skin Biopsy Specimens to Identify the Cause of Cellulitis

2015 ◽  
pp. civ655 ◽  
Author(s):  
Jonathan G. Crisp ◽  
Sukhjit S. Takhar ◽  
Gregory J. Moran ◽  
Anusha Krishnadasan ◽  
Scot E. Dowd ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Skin Biopsy ◽  
Chain Reaction ◽  
Biopsy Specimens ◽  
Polymerase Chain
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