Quantitative fluorescence measurements from tissue using confocal detection

1997 ◽  
Author(s):  
Brian W. Pogue ◽  
Tayyaba Hasan
Keyword(s):  
Fluorescence Measurements ◽  
Quantitative Fluorescence ◽  
Confocal Detection

scholarly journals Quantitative fluorescence in intracranial tumor: implications for ALA-induced PpIX as an intraoperative biomarker

2011 ◽  
Vol 115 (1) ◽  
pp. 11-17 ◽  
Author(s):  
Pablo A. Valdés ◽  
Frederic Leblond ◽  
Anthony Kim ◽  
Brent T. Harris ◽  
Brian C. Wilson ◽  
...  
Keyword(s):  
Brain Tissue ◽  
Fluorescence Imaging ◽  
Low Grade ◽  
Normal Brain ◽  
Intracranial Tumors ◽  
Neoplastic Tissue ◽  
Fluorescence Measurements ◽  
Significant Difference ◽  
Quantitative Fluorescence

Object Accurate discrimination between tumor and normal tissue is crucial for optimal tumor resection. Qualitative fluorescence of protoporphyrin IX (PpIX), synthesized endogenously following δ-aminolevulinic acid (ALA) administration, has been used for this purpose in high-grade glioma (HGG). The authors show that diagnostically significant but visually imperceptible concentrations of PpIX can be quantitatively measured in vivo and used to discriminate normal from neoplastic brain tissue across a range of tumor histologies. Methods The authors studied 14 patients with diagnoses of low-grade glioma (LGG), HGG, meningioma, and metastasis under an institutional review board–approved protocol for fluorescence-guided resection. The primary aim of the study was to compare the diagnostic capabilities of a highly sensitive, spectrally resolved quantitative fluorescence approach to conventional fluorescence imaging for detection of neoplastic tissue in vivo. Results A significant difference in the quantitative measurements of PpIX concentration occurred in all tumor groups compared with normal brain tissue. Receiver operating characteristic (ROC) curve analysis of PpIX concentration as a diagnostic variable for detection of neoplastic tissue yielded a classification efficiency of 87% (AUC = 0.95, specificity = 92%, sensitivity = 84%) compared with 66% (AUC = 0.73, specificity = 100%, sensitivity = 47%) for conventional fluorescence imaging (p < 0.0001). More than 81% (57 of 70) of the quantitative fluorescence measurements that were below the threshold of the surgeon's visual perception were classified correctly in an analysis of all tumors. Conclusions These findings are clinically profound because they demonstrate that ALA-induced PpIX is a targeting biomarker for a variety of intracranial tumors beyond HGGs. This study is the first to measure quantitative ALA-induced PpIX concentrations in vivo, and the results have broad implications for guidance during resection of intracranial tumors.

Quantitative fluorescence measurements of ao-stained normal and malignant bladder cells

1983 ◽  
Vol 31 (5) ◽  
pp. 577-585 ◽  
Author(s):  
George P. Hemstreet ◽  
Seymour S. West ◽  
W. Lamar Weems ◽  
Candace K. Echols ◽  
Sandra McFarland ◽  
...  
Keyword(s):  
Fluorescence Measurements ◽  
Bladder Cells ◽  
Quantitative Fluorescence

Toward quantitative fluorescence measurements with multicolor flow cytometry

2008 ◽  
Vol 73A (4) ◽  
pp. 279-288 ◽  
Author(s):  
Lili Wang ◽  
Adolfas K. Gaigalas ◽  
Gerald Marti ◽  
Fatima Abbasi ◽  
Robert A. Hoffman
Keyword(s):  
Flow Cytometry ◽  
Multicolor Flow Cytometry ◽  
Fluorescence Measurements ◽  
Quantitative Fluorescence
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