Influence of He-Ne laser radiation on biogenic amines content and cytochemical parameters of polymorphonuclear leukocytes in short-term stress

1997 ◽  
Author(s):  
Gregory E. Brill ◽  
Gennady A. Dobrovolsky ◽  
Tatyana P. Romanova ◽  
Svetlana G. Porozova ◽  
Alexander G. Brill
Keyword(s):  
Laser Radiation ◽  
Biogenic Amines ◽  
Polymorphonuclear Leukocytes ◽  
Short Term ◽  
Cytochemical Parameters

ESTIMATION OF THE SAFETY OF LOW-INTENSITY LASER RADIATION IN TREATMENT OF PATIENTS WITH PROFESSIONAL POLYNEUROPATHIES OF THE UPPER LIMBS

2021 ◽  
Author(s):  
S.V. Grebenkov ◽  
◽  
N.Y. Malkova ◽  
O.A. Kochetova ◽  
Keyword(s):  
Laser Radiation ◽  
Side Effects ◽  
Upper Extremities ◽  
Projection Area ◽  
Outpatient Basis ◽  
Short Term ◽  
Low Intensity ◽  
Number Of Patients ◽  
Intensity Laser ◽  
Local Side

Abstract: The inclusion of low-intensity laser radiation in the complex treatment of professional polyneuropathies of the upper extremities, due to its numerous biological effects, contributes to a statistically significant reduction in pain in the hands of patients, and improves the conduction of nerve impulses along the peripheral nerves of the hands. In addition to describing the positive clinical and neurophysiological trends achieved during treatment, the issue of the safety of the developed therapy method is important. The aim of the study is to assess the safety of the proposed method of low-intensity laser therapy (LILT) in patients with professional polyneuropathies of the upper extremities. Material and methods: on the basis of the department of occupational pathology, 116 patients (60 men, 56 women) with occupational polyneuropathies of the upper extremities were treated using LILT. Therapeutic measures were carried out using a semiconductor laser apparatus for red spectrum radiation (650 nm) with fiber-optic light guides for percutaneous therapy "ALP-01-Laton" in the sitting position of patients. Diffuse scattered laser radiation of the red region of the spectrum with an energy illumination of 0.7-1.1 mW / cm2 directly affected the projection area of the exit of the median nerve from the carpal tunnel to the hand. There were 5-10 procedures per course, the energy exposure of laser radiation and the number of sessions were determined individually. Results: To ensure the safety of the therapy, patients were included in the study strictly in accordance with the developed inclusion and non-inclusion criteria. Patients were treated in a specially equipped room with the use of personal protective equipment; safety rules were followed during the operation of the device. During treatment, all 116 patients treated with LILT did not have systemic side effects. A number of patients developed short-term complaints associated with local reactions: short-term tingling sensations and "goose bumps" in the hands, warmth, slight burning sensation, etc. All recorded local side effects occurred directly during LILT-procedures and were completely stopped within 1-5 minutes after their completion. In general, LILT-exposure did not cause serious side effects and was well tolerated by patients. 13.5% of patients noted a decrease in pain in the arms directly during the LILT treatment procedures. Conclusion. The developed method of LILT of professional polyneuropathies of the upper extremities is simple and convenient for use in clinical practice: it is applicable both in a hospital and on an outpatient basis. LILT has a limited list of contraindications, and is generally well tolerated by patients with minimal local side effects.

scholarly journals Impact of M49, Mrp, Enn, and C5a Peptidase Proteins on Colonization of the Mouse Oral Mucosa by Streptococcus pyogenes

1998 ◽  
Vol 66 (11) ◽  
pp. 5399-5405 ◽  
Author(s):  
Yinduo Ji ◽  
Norbert Schnitzler ◽  
Eric DeMaster ◽  
Patrick Cleary
Keyword(s):  
Oral Mucosa ◽  
Streptococcus Pyogenes ◽  
Polymorphonuclear Leukocytes ◽  
Group A Streptococcus ◽  
M Protein ◽  
Insertion Mutation ◽  
Short Term ◽  
Group A ◽  
Isogenic Strains

ABSTRACT Resistance to phagocytosis is a hallmark of virulentStreptococcus pyogenes (group A streptococcus). Surface-bound C5a peptidase reduces recruitment of phagocytes to the site of infection, and hyaluronic acid capsules and/or the M protein limit the uptake of streptococci. In this study the relative impact of M and M-like proteins and the C5a peptidase on the virulence of a serotype M49 strain was assessed. The capacities of isogenic strains with an insertion mutation in emm49; with a deletion mutation in scpA49 (C5a peptidase gene); and with a deletion that removes all three M-like genes, mrp49,emm49, and enn49, to colonize mice and resist phagocytosis were compared. Experiments confirmed results obtained in an earlier study, which showed that the M49 protein was not required for in vitro resistance to phagocytosis, and also showed that the M protein was not required for colonization of mice. Failure to produce all three M-like proteins, M49, Mrp, and Enn49, significantly reduced the ability of these streptococci to resist phagocytosis in vitro but did not significantly alter the persistence of streptococci on the oral mucosa. In vitro experiments indicate that M+ streptococci are phagocytized by polymorphonuclear leukocytes that have been activated with phorbol-12-myristate 13-acetate or recombinant human C5a. This observation may explain the finding that expression of M49 protein is not essential for short-term colonization of the mouse oral mucosa.

Cholesterol feeding enhances vasoconstrictor effects of products from rabbit polymorphonuclear leukocytes

1995 ◽  
Vol 269 (1) ◽  
pp. H1-H6
Author(s):  
J. L. Hart ◽  
C. G. Sobey ◽  
O. L. Woodman
Keyword(s):  
Polymorphonuclear Leukocytes ◽  
Plasma Cholesterol ◽  
Rabbit Aorta ◽  
Cholesterol Diet ◽  
Cholesterol Feeding ◽  
Short Term ◽  
Receptor Reserve ◽  
Atherosclerotic Lesions ◽  
Cholesterol Levels ◽  
Before And After

We have studied the vasoactive properties of products released from rabbit polymorphonuclear leukocytes (PMNs) before and after short-term (4 and 8 wk) dietary supplementation with 1% cholesterol. Plasma cholesterol levels were similar after 4 and 8 wk of cholesterol diet, whereas gross atherosclerotic lesions were present at 4 wk but significantly more extensive after 8 wk. PMN products from all rabbits caused endothelium-dependent contraction of isolated, control (nonatherosclerotic) rabbit aorta submaximally contracted with phenylephrine. However, both 4 and 8 wk of cholesterol feeding resulted in equivalent contractions by PMN products, which were significantly greater than contractions by control PMNs. Endothelium-dependent contraction (by PMN products) and relaxation (by acetylcholine) were attenuated by 8 wk of cholesterol feeding. PMN products attenuated acetylcholine-induced relaxation of aorta from cholesterol-fed rabbits and of control aorta treated with phenoxybenzamine to reduce muscarinic receptor reserve. We conclude that elevation of plasma cholesterol results in increased release of a PMN product(s) that causes endothelium-dependent constriction.

Influence of low-power laser radiation on mast cells function and biogenic amines content in stress

1997 ◽  
Author(s):  
Gregory E. Brill ◽  
Gennady A. Dobrovolsky ◽  
Tatyana P. Romanova ◽  
Alexander G. Brill
Keyword(s):  
Laser Radiation ◽  
Mast Cells ◽  
Low Power ◽  
Biogenic Amines ◽  
Power Laser

scholarly journals STUDIES ON THE LOCALIZATION OF CIRCULATING ANTIGEN-ANTIBODY COMPLEXES AND OTHER MACROMOLECULES IN VESSELS

1963 ◽  
Vol 118 (4) ◽  
pp. 489-502 ◽  
Author(s):  
Charles G. Cochrane
Keyword(s):  
Basement Membrane ◽  
Anaphylactic Shock ◽  
Vessel Wall ◽  
Polymorphonuclear Leukocytes ◽  
Blood Stream ◽  
Soluble Antigen ◽  
Short Term ◽  
Circulating Antigen ◽  
Vessel Walls ◽  
Antigen Antibody

A short term model in which circulating antigen-antibody complexes and host complement localized in vessel walls of guinea pigs was analyzed. Localization was accomplished by subjecting the animals to anaphylactic shock. The circulating macromolecules, such as antigen-antibody complexes, appeared to localize by being trapped in the vessel wall along the basement membrane that acted as a filter during a state of increased permeability of the vessel. It was suggested that this point of localization between the endothelial cell and the basement membrane may well represent the earliest focus of inflammation in diseases caused by the deposition of injurious macromolecules such as soluble antigen-antibody complexes from the blood stream. Complexes localized in the vessel walls did not provoke Arthus-type vasculonecrotic reactions even though in both these vessels and in cutaneous Arthus reactions antibody, antigen, and host complement (C'3c) were deposited in the vessel walls. The possibility was presented that since circulating macromolecules and probably complexes deposited in (a) relatively small amounts, and (b) in a position beneath endothelial cells, they were not strongly chemotactic toward circulating polymorphonuclear leukocytes. Vasculonecrotic reactions, therefore, were not observed. It was brought out that this may be similar to the situation in glomerulonephritis induced by localized immune complexes, in which severe necrosis is not observed. In the Arthus vascular reaction, host complement was found microscopically accumulated with the immune reactants in affected vessel walls.

Modification of lipid peroxidation by HeNe-laser radiation in short-term immobilization stress

1993 ◽  
Author(s):  
Gregory E. Brill ◽  
Olga V. Proshina ◽  
Valentina N. Zhigalina ◽  
Lyudmila S. Filimonovskaya
Keyword(s):  
Lipid Peroxidation ◽  
Laser Radiation ◽  
Immobilization Stress ◽  
Short Term ◽  
Hene Laser

Liquid Chromatographic Method for Determination of Biogenic Amines in Fish and Fish Products

1995 ◽  
Vol 78 (4) ◽  
pp. 1045-1050 ◽  
Author(s):  
María Teresa Veciana-Nogues ◽  
Teresa Hernandez-Jover ◽  
Abel Marine-Font ◽  
María Del Carmen Vedal-Carou
Keyword(s):  
Biogenic Amines ◽  
Chromatographic Method ◽  
Frozen Storage ◽  
Short Term ◽  
Fish Products ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic ◽  
Postcolumn Derivatization

Abstract A liquid chromatographic method with postcolumn derivatization is described for determination of biogenic amines in fish and fish products. Histamine, tyramine, serotonin, β-phenylethylamine, tryptamine, putrescine, cadaverine, agmatine, spermine, and spermidine can be determined in less than 60 min. Routine sample preinjection treatment implies only 2 extractions with 0.6N perchloric acid and filtration through a 0.45 μm filter. Lack of interferences from volatile amines, amino acids, and dipeptides was verified. Results of reliability study were satisfactory. The proposed method was linear for each amine between 0.25 and 8.00 mg/L. Average recoveries ranged from 92 to 103%. Precisions (coefficients of variation) ranged from 0.70 to 9.75%. Determination limits were ≤1 mg/kg. A modification in LC conditions was necessary to apply the method to ripened fish products to avoid interferences from food matrixes. In addition, stability of biogenic amines in fresh anchovies and hake during short-term frozen storage was studied. Results showed that, except for agmatine, frozen storage is suitable for keeping samples before analysis.

scholarly journals Method development to secure the stability of the parameters of pulsed and continuous radiation in laser systems with semiconductor pumping

2021 ◽  
Vol 2086 (1) ◽  
pp. 012126
Author(s):  
S R Abdurakhmanova ◽  
G D Bukharov ◽  
Z S Gheisser ◽  
V V Davydov ◽  
V Yu Rud
Keyword(s):  
Laser Radiation ◽  
Method Development ◽  
Pulsed Laser ◽  
High Accuracy ◽  
Short Term ◽  
Pulsed Laser Radiation ◽  
Main Requirement ◽  
Continuous Radiation ◽  
Laser Systems ◽  
The Stability

Abstract The necessity of increasing the stability of the parameters of pulsed and cw laser radiation when operating at peak powers is substantiated. Particular attention is paid to the parameters of pulsed laser radiation in systems with semiconductor pumping. The main requirement in this case is the repeatability of the parameters of the output pulses with high accuracy. This condition is ensured by the stability of the diode supply drivers, especially at peak powers, which are required for scientific research. It was found that even a short-term excess (about 1 ms) of the upper range of the permissible current leads to damage to the laser diode, which instantly degrades the parameters of laser radiation. The developed technique can significantly reduce the likelihood of these phenomena and increase the reliability of the laser.

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