Speckle noise suppression of digital holographic microscopy with diffusion glass rotation

2021 ◽  
Author(s):  
Bingcai Liu ◽  
Rui Niu ◽  
Ailing Tian ◽  
Hongjun Wang ◽  
Xueliang Zhu ◽  
...  
2021 ◽  
Vol 484 ◽  
pp. 126701
Author(s):  
Chi-Ching Chang ◽  
Huang-Tian Chan ◽  
Min-Tzung Shiu ◽  
Yang-Kun Chew ◽  
Mark Shey Sussman

2021 ◽  
Vol 9 ◽  
Author(s):  
Kequn Zhuo ◽  
Yu Wang ◽  
Yang Wang ◽  
Kai Wen ◽  
Min Liu ◽  
...  

This paper presents a partially coherent point-diffraction digital holographic microscopy (PC-pDHM) prototype and demonstrates its application in label-free imaging of the dynamic processes of live cells. In PC-pDHM, the light scattered by a rotating diffuser is coupled into a multi-mode fiber, and the output light is used as the partially coherent illumination (PCI), which reduces the speckle noise significantly in PC-pDHM. A polarization-grating is used to remold the object and the reference waves, and the fringe contrast of the generated hologram can be adjusted by changing the polarization of the illumination wave. Using the PC-pDHM prototype, transparent samples and notably the dynamic processes of live cells were imaged with high contrast and in a label-free manner, discovering the pathological mechanisms of biology in the cellular and sub-cellular levels.


2021 ◽  
Vol 84 ◽  
pp. 200-207
Author(s):  
Jiansen Pan ◽  
Qingmei Peng ◽  
Guoliang Zhang ◽  
Qingyi Xie ◽  
Xiangjun Gong ◽  
...  

Photonics ◽  
2021 ◽  
Vol 8 (7) ◽  
pp. 276
Author(s):  
Yun Liu ◽  
Peihua Bu ◽  
Mingxing Jiao ◽  
Junhong Xing ◽  
Ke Kou ◽  
...  

The coherent noise always exists in digital holographic microscopy due to the laser source, degrading the image quality. A method of speckle suppression using the dynamic speckle illumination, produced by double-moving diffusers, is presented in digital holographic microscopy. The space–time correlation functions are theoretically analyzed from the statistics distribution in the doubly and singly scattered system, respectively. The configuration of double-moving diffusers is demonstrated to have better performance in speckle suppression compared with the single diffuser and moving-static double diffusers cases. The experiment results verify the feasibility of the approach. The presented approach only requires a single shot interferogram to realize the speckle reduction, accordingly it has the potential application in real-time measurement.


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