Deep learning for tracking of intracellular vesicles in time-lapse microscopy images

2021 ◽  
Author(s):  
Zhichao Liu ◽  
Luhong Jin ◽  
Alexander Nedzved ◽  
Sergey Ablameyko ◽  
Yingke Xu
Keyword(s):  
Deep Learning ◽  
Time Lapse ◽  
Time Lapse Microscopy ◽  
Intracellular Vesicles ◽  
Microscopy Images

scholarly journals SpinX: Time-resolved 3D Analysis of Mitotic Spindle Dynamics using Deep Learning Techniques and Mathematical Modelling

2021 ◽  
Author(s):  
David Dang ◽  
Christoforos Efstathiou ◽  
Dijue Sun ◽  
Nishanth Sastry ◽  
Viji M Draviam
Keyword(s):  
Deep Learning ◽  
Mathematical Object ◽  
Time Lapse ◽  
Cell Cortex ◽  
3D Analysis ◽  
Time Resolved ◽  
3 Dimensional ◽  
Time Lapse Microscopy ◽  
Exciting Opportunity ◽  
Spindle Dynamics

Time-lapse microscopy movies have transformed the study of subcellular dynamics. However, manual analysis of movies can introduce bias and variability, obscuring important insights. While automation can overcome such limitations, spatial and temporal discontinuities in time-lapse movies render methods such as object segmentation and tracking difficult. Here we present SpinX, a framework for reconstructing gaps between successive frames by combining Deep Learning and mathematical object modelling. By incorporating expert feedback through selective annotations, SpinX identifies subcellular structures, despite confounding neighbour-cell information, non-uniform illumination and variable marker intensities. The automation and continuity introduced allows precise 3-Dimensional tracking and analysis of spindle movements with respect to the cell cortex for the first time. We demonstrate the utility of SpinX using distinct spindle markers and drug treatments. In summary, SpinX provides an exciting opportunity to study spindle dynamics in a sophisticated way, creating a framework for step changes in studies using time-lapse microscopy.

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