FDISCO+: a clearing method for robust fluorescence preservation of cleared samples

2021 ◽  
Author(s):  
Peng Wan ◽  
Yusha Li ◽  
Jingtan Zhu ◽  
Jianyi Xu ◽  
Xiaomei Liu ◽  
...  
Keyword(s):  
Clearing Method

Flexible block offers and a three-stage market clearing method for distribution-level electricity markets with grid limits

2021 ◽  
Vol 130 ◽  
pp. 106985
Author(s):  
Shaojun Huang ◽  
Yuming Zhao ◽  
Konstantin Filonenko ◽  
Yun Wang ◽  
Tianlong Xiong ◽  
...  
Keyword(s):  
Electricity Markets ◽  
Market Clearing ◽  
Clearing Method

Capacity Clearing Method Based on Reliability Pricing Model

2021 ◽  
Author(s):  
Tao Zhu ◽  
Yi Wang ◽  
Haohao Wang ◽  
Mingxing Wu ◽  
Qing Chen ◽  
...  
Keyword(s):  
Pricing Model ◽  
Clearing Method

scholarly journals A Hydrophobic Tissue Clearing Method for Rat Brain Tissue

10.3791/61821 ◽  
2020 ◽  
Author(s):  
Kristin N. Kirchner ◽  
Hailong Li ◽  
Adam R. Denton ◽  
Steven B. Harrod ◽  
Charles F. Mactutus ◽  
...  
Keyword(s):  
Rat Brain ◽  
Brain Tissue ◽  
Tissue Clearing ◽  
Clearing Method ◽  
Rat Brain Tissue

scholarly journals EZ Clear for simple, rapid, and robust mouse whole organ clearing

2022 ◽  
Author(s):  
Chih-Wei Hsu ◽  
Juan Cerda ◽  
Jason M Kirk ◽  
Williamson D. Turner ◽  
Tara L. Rasmussen ◽  
...  
Keyword(s):  
Biomedical Research ◽  
Room Temperature ◽  
Adult Mouse ◽  
Dimensional Space ◽  
Three Dimensional ◽  
Fluorescence Signal ◽  
Tissue Architecture ◽  
Tissue Clearing ◽  
Clearing Method ◽  
Three Dimensional Space

Tissue clearing for whole organ cell profiling has revolutionized biology and imaging for exploration of organs in three-dimensional space without compromising tissue architecture. But complicated, laborious procedures, or expensive equipment, as well as the use of hazardous, organic solvents prevents the widespread adoption of these methods. Here we report a simple and rapid tissue clearing method, EZ Clear, that can clear whole adult mouse organs in 48 hours in just three simple steps. Samples stay at room temperature and remain hydrated throughout the clearing process, preserving endogenous and synthetic fluorescence, without altering sample size. After wholemount clearing and imaging, EZ Cleared samples can be further processed for downstream embedding and cryosectioning followed by standard histology or immunostaining, without loss of endogenous or synthetic fluorescence signal. Overall, the simplicity, speed, and flexibility of EZ Clear make it easy to adopt and apply to diverse approaches in biomedical research.

scholarly journals FDISCO+: a clearing method for robust fluorescence preservation of cleared samples

2021 ◽  
Vol 8 (03) ◽  
Author(s):  
Peng Wan ◽  
Yusha Li ◽  
Jingtan Zhu ◽  
Jianyi Xu ◽  
Xiaomei Liu ◽  
...  
Keyword(s):  
Clearing Method

scholarly journals Dust Pollution Reduced Stomatal Conductance and Photosynthetic Pigments of Selected Medicinal Plants Growing at Lokpa Ukwu Quarry Site in Abia State, Nigeria

2020 ◽  
pp. 1-11
Author(s):  
C. E. Ogbonna ◽  
F. I. Nwafor ◽  
N. O. Nweze
Keyword(s):  
Stomatal Conductance ◽  
Medicinal Plants ◽  
Photosynthetic Pigments ◽  
Total Chlorophyll ◽  
Negative Effects ◽  
Dust Pollution ◽  
Standard Procedures ◽  
Maximum Conductance ◽  
Clearing Method ◽  
Β Carotene

Aims: We investigated the influence of dust pollution on stomatal conductance and photosynthetic pigments in some medicinal plants growing at Lokpa Ukwu quarry site, Abia State, Nigeria. Place and Duration of Study: Samples were collected from Lokpa Ukwu, Abia State while laboratory analyses were carried out in the Department of Pharmacognosy and Environmental Medicines, University of Nigeria, Nsukka between February and April, 2019. Methodology: A total of nine (9) plants were sampled for the study. Leaf epidermises were prepared by clearing method and stomata were observed and studied quantitatively. Stomatal conductance was estimated from the anatomical variables following standard procedures. Total chlorophyll and β-carotene contents were also analysed and compared with control groups. Results: We observed some physiological changes in the plants from dust-polluted site such as stretched epidermal cells, deformed stomata and plasmolysed guard cells. It reduced the potential conductance indices (PCI) of the plants by 87.4% in Aspilia africana and 67% in Chromolaena odorata. The least reduction in PCI was observed in Celosia trigyna (7.2%). Operating conductance (gop) and maximum conductance (gmax) were reduced by 69.2% and 72.3% in C. odorata and A. africana respectively. Celosia trigyna was least affected with percentage reductions of 18.3% and 1.4% for gop and gmax respectively. Reduction in PCI and gmax followed the order: C. trigyna ˂ C. papaya ˂ P. discoideus ˂ D. oliveri ˂ T. rhomboidea ˂ T. orientalis ˂ V. doniana ˂ C. odorata ˂ A. africana. Total chlorophyll and β-carotene contents were reduced the most in V. doniana (45.73%) and C. odorata (40.31%) respectively and least reduced in T. orientalis by 19.54% and 13.24% respectively. Conclusion: Our findings validate previous reports of negative effects of dust pollution from quarry industries on both humans and plants alike.

scholarly journals Studies on the lymphnode metastasis of gastric cancer and the sinus histiocytosis of the lymphnode by the clearing method.

1987 ◽  
Vol 20 (8) ◽  
pp. 1837-1843
Author(s):  
Akira TANAKA ◽  
Haruhiko INUFUSA ◽  
Hiromi TAKAGI ◽  
Masaki HATTA ◽  
Tetsuhiko NAKAMURA ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Sinus Histiocytosis ◽  
Lymphnode Metastasis ◽  
Clearing Method
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