Stitching technique applied to UV intrinsic fluorescence imaging in an in vivo wound-healing model

2021 ◽  
Author(s):  
Axel Aparicio-Hernandez ◽  
Mariana Alfaro-Gomez ◽  
Enoch Gutierrez-Herrera
Keyword(s):  
Wound Healing ◽  
Fluorescence Imaging ◽  
Intrinsic Fluorescence ◽  
Wound Healing Model ◽  
Healing Model

The Chick Embryo Chorioallantoic Membrane as an in vivo Wound Healing Model

1996 ◽  
Vol 192 (10) ◽  
pp. 1068-1076 ◽  
Author(s):  
D. Ribatti ◽  
A. Vacca ◽  
G. Ranieri ◽  
S. Sorino ◽  
L. Roncali
Keyword(s):  
Wound Healing ◽  
Chick Embryo ◽  
Chorioallantoic Membrane ◽  
Chick Embryo Chorioallantoic Membrane ◽  
Wound Healing Model ◽  
Healing Model

scholarly journals Single-Cell Gene Expression Analysis and Evaluation of the Therapeutic Function of Murine Adipose-Derived Stromal Cells (ASCs) from the Subcutaneous and Visceral Compartment

2018 ◽  
Vol 2018 ◽  
pp. 1-9
Author(s):  
Dominik Pförringer ◽  
Matthias M. Aitzetmüller ◽  
Elizabeth A. Brett ◽  
Khosrow S. Houschyar ◽  
Richard Schäfer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wound Healing ◽  
Single Cell ◽  
Stromal Cells ◽  
Single Cell Level ◽  
Cell Level ◽  
Adipose Derived Stromal Cells ◽  
Wound Healing Model ◽  
Healing Model

Introduction. Adipose-derived stromal cells (ASCs) are a promising resource for wound healing and tissue regeneration because of their multipotent properties and cytokine secretion. ASCs are typically isolated from the subcutaneous fat compartment, but can also be obtained from visceral adipose tissue. The data on their equivalence diverges. The present study analyzes the cell-specific gene expression profiles and functional differences of ASCs derived from the subcutaneous (S-ASCs) and the visceral (V-ASCs) compartment. Material and Methods. Subcutaneous and visceral ASCs were obtained from mouse inguinal fat and omentum. The transcriptional profiles of the ASCs were compared on single-cell level. S-ASCs and V-ASCs were then compared in a murine wound healing model to evaluate their regenerative functionality. Results. On a single-cell level, S-ASCs and V-ASCs displayed distinct transcriptional profiles. Specifically, significant differences were detected in genes associated with neoangiogenesis and tissue remodeling (for example, Ccl2, Hif1α, Fgf7, and Igf). In addition, a different subpopulation ecology could be identified employing a cluster model. Nevertheless, both S-ASCs and V-ASCs induced accelerated healing rates and neoangiogenesis in a mouse wound healing model. Conclusion. With similar therapeutic potential in vivo, the significantly different gene expression patterns of ASCs from the subcutaneous and visceral compartments suggest different signaling pathways underlying their efficacy. This study clearly demonstrates that review of transcriptional results in vivo is advisable to confirm the tentative effect of cell therapies.

scholarly journals Effect of Unloading Condition on the Healing Process and Effectiveness of Platelet Rich Plasma as a Countermeasure: Study on In Vivo and In Vitro Wound Healing Models

2020 ◽  
Vol 21 (2) ◽  
pp. 407 ◽  
Author(s):  
Francesca Cialdai ◽  
Alessandra Colciago ◽  
Desiré Pantalone ◽  
Angela Maria Rizzo ◽  
Stefania Zava ◽  
...  
Keyword(s):  
Wound Healing ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Physical Factors ◽  
Medical Evacuation ◽  
Fibroblast Migration ◽  
Wound Healing Model ◽  
Healing Model

Wound healing is a very complex process that allows organisms to survive injuries. It is strictly regulated by a number of biochemical and physical factors, mechanical forces included. Studying wound healing in space is interesting for two main reasons: (i) defining tools, procedures, and protocols to manage serious wounds and burns eventually occurring in future long-lasting space exploration missions, without the possibility of timely medical evacuation to Earth; (ii) understanding the role of gravity and mechanical factors in the healing process and scarring, thus contributing to unravelling the mechanisms underlying the switching between perfect regeneration and imperfect repair with scarring. In the study presented here, a new in vivo sutured wound healing model in the leech (Hirudo medicinalis) has been used to evaluate the effect of unloading conditions on the healing process and the effectiveness of platelet rich plasma (PRP) as a countermeasure. The results reveal that microgravity caused a healing delay and structural alterations in the repair tissue, which were prevented by PRP treatment. Moreover, investigating the effects of microgravity and PRP on an in vitro wound healing model, it was found that PRP is able to counteract the microgravity-induced impairment in fibroblast migration to the wound site. This could be one of the mechanisms underlying the effectiveness of PRP in preventing healing impairment in unloading conditions.

148
In Vivo Adenoviral Gene Transfer of SPARC in a Skin-Humanized Mouse Wound Healing Model

2008 ◽  
Vol 13 (2) ◽  
pp. A28-A48
Author(s):  
M.J. Escámez ◽  
M. Carretero ◽  
F. Prada ◽  
F. Larcher ◽  
M. García ◽  
...  
Keyword(s):  
Wound Healing ◽  
Gene Transfer ◽  
Humanized Mouse ◽  
Adenoviral Gene Transfer ◽  
Wound Healing Model ◽  
Healing Model

Validation of an in vivo Wound Healing Model for the Quantification of Pharmacological Effects on Epidermal Regeneration

Dermatology ◽  
1995 ◽  
Vol 190 (2) ◽  
pp. 136-141 ◽  
Author(s):  
J.J. Lévy ◽  
J. von Rosen ◽  
J. Gaßmüller ◽  
R. Kleine Kuhlmann ◽  
L. Lange
Keyword(s):  
Wound Healing ◽  
Pharmacological Effects ◽  
Wound Healing Model ◽  
Epidermal Regeneration ◽  
Healing Model

scholarly journals Galvanic microparticles increase migration of human dermal fibroblasts in a wound-healing model via reactive oxygen species pathway

2014 ◽  
Vol 320 (1) ◽  
pp. 79-91 ◽  
Author(s):  
Nina Tandon ◽  
Elisa Cimetta ◽  
Aranzazu Villasante ◽  
Nicolette Kupferstein ◽  
Michael D. Southall ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Wound Healing ◽  
Reactive Oxygen ◽  
Dermal Fibroblasts ◽  
Oxygen Species ◽  
Human Dermal Fibroblasts ◽  
Wound Healing Model ◽  
Healing Model

scholarly journals Transcriptomic Analysis of a Diabetic Skin-Humanized Mouse Model Dissects Molecular Pathways Underlying the Delayed Wound Healing Response

Genes ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 47
Author(s):  
Carlos León ◽  
Francisco García-García ◽  
Sara Llames ◽  
Eva García-Pérez ◽  
Marta Carretero ◽  
...  
Keyword(s):  
Wound Healing ◽  
Functional Enrichment ◽  
Preclinical Model ◽  
Published Data ◽  
Diabetic Patients ◽  
Ulcer Formation ◽  
Histological Features ◽  
Depth Analysis ◽  
Wound Healing Model ◽  
Healing Model

Defective healing leading to cutaneous ulcer formation is one of the most feared complications of diabetes due to its consequences on patients’ quality of life and on the healthcare system. A more in-depth analysis of the underlying molecular pathophysiology is required to develop effective healing-promoting therapies for those patients. Major architectural and functional differences with human epidermis limit extrapolation of results coming from rodents and other small mammal-healing models. Therefore, the search for reliable humanized models has become mandatory. Previously, we developed a diabetes-induced delayed humanized wound healing model that faithfully recapitulated the major histological features of such skin repair-deficient condition. Herein, we present the results of a transcriptomic and functional enrichment analysis followed by a mechanistic analysis performed in such humanized wound healing model. The deregulation of genes implicated in functions such as angiogenesis, apoptosis, and inflammatory signaling processes were evidenced, confirming published data in diabetic patients that in fact might also underlie some of the histological features previously reported in the delayed skin-humanized healing model. Altogether, these molecular findings support the utility of such preclinical model as a valuable tool to gain insight into the molecular basis of the delayed diabetic healing with potential impact in the translational medicine field.

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