SEPSIS biomarker detection through lensless fiber-based planar antennas

2021 ◽  
Author(s):  
Pietro Ernesto Lombardi ◽  
Ambra E. Giannetti ◽  
Paolo Cecchi ◽  
Francesco E. Chiavaioli ◽  
Steffen Howitz ◽  
...  
Keyword(s):  
Biomarker Detection ◽  
Planar Antennas

SEPSIS biomarker detection through fiber-based planar antennas (Conference Presentation)

2020 ◽  
Author(s):  
Ambra Giannetti ◽  
Paolo Cecchi ◽  
Francesco Chiavaioli ◽  
Steffen Howitz ◽  
Pietro Lombardi ◽  
...  
Keyword(s):  
Biomarker Detection ◽  
Planar Antennas

scholarly journals Single Step In Situ Detection of Surface Protein and MicroRNA in Clustered Extracellular Vesicles Using Flow Cytometry

2021 ◽  
Vol 10 (2) ◽  
pp. 319
Author(s):  
Hee Cheol Yang ◽  
Won Jong Rhee
Keyword(s):  
Extracellular Vesicles ◽  
Liquid Biopsy ◽  
Molecular Beacon ◽  
Disease Diagnosis ◽  
Single Step ◽  
Flow Cytometer ◽  
Surface Proteins ◽  
Biomarker Detection ◽  
In Situ Detection

Because cancers are heterogeneous, it is evident that multiplexed detection is required to achieve disease diagnosis with high accuracy and specificity. Extracellular vesicles (EVs) have been a subject of great interest as sources of novel biomarkers for cancer liquid biopsy. However, EVs are nano-sized particles that are difficult to handle; thus, it is necessary to develop a method that enables efficient and straightforward EV biomarker detection. In the present study, we developed a method for single step in situ detection of EV surface proteins and inner miRNAs simultaneously using a flow cytometer. CD63 antibody and molecular beacon-21 were investigated for multiplexed biomarker detection in normal and cancer EVs. A phospholipid-polymer-phospholipid conjugate was introduced to induce clustering of the EVs analyzed using nanoparticle tracking analysis, which enhanced the detection signals. As a result, the method could detect and distinguish cancer cell-derived EVs using a flow cytometer. Thus, single step in situ detection of multiple EV biomarkers using a flow cytometer can be applied as a simple, labor- and time-saving, non-invasive liquid biopsy for the diagnosis of various diseases, including cancer.

scholarly journals Characteristics and Clinical Application of Extracellular Vesicle-Derived DNA

Cancers ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 3827
Author(s):  
Jae Young Hur ◽  
Kye Young Lee
Keyword(s):  
Therapeutic Potential ◽  
Extracellular Vesicle ◽  
Clinical Settings ◽  
Biomarker Detection ◽  
Diagnosis And Prognosis ◽  
Double Stranded Dna ◽  
Fundamental Research ◽  
The Stability ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing

Extracellular vesicles (EVs) carry RNA, proteins, lipids, and diverse biomolecules for intercellular communication. Recent studies have reported that EVs contain double-stranded DNA (dsDNA) and oncogenic mutant DNA. The advantage of EV-derived DNA (EV DNA) over cell-free DNA (cfDNA) is the stability achieved through the encapsulation in the lipid bilayer of EVs, which protects EV DNA from degradation by external factors. The existence of DNA and its stability make EVs a useful source of biomarkers. However, fundamental research on EV DNA remains limited, and many aspects of EV DNA are poorly understood. This review examines the known characteristics of EV DNA, biogenesis of DNA-containing EVs, methylation, and next-generation sequencing (NGS) analysis using EV DNA for biomarker detection. On the basis of this knowledge, this review explores how EV DNA can be incorporated into diagnosis and prognosis in clinical settings, as well as gene transfer of EV DNA and its therapeutic potential.

Superradiative Plasmonic Nanoantenna Biosensors Enable Sensitive Immunoassay Using Naked Eyes

Nanoscale ◽  
2021 ◽  
Author(s):  
Yang Xiong ◽  
Tong Fu ◽  
Daxiao Zhang ◽  
Shunping Zhang ◽  
Hongxing Xu
Keyword(s):  
Disease Prediction ◽  
Biomarker Detection

Easy-to-use and sensitive quantification of biomarkers has great significance in disease prediction, diagnosis, and monitoring. Here, we report a biosensor for simple and sensitive biomarker detection based on the strong...

Precise and Convenient Size Barcode on Microfluidic Chip for Multiplex Biomarker Detection

The Analyst ◽  
2021 ◽  
Author(s):  
Man Tang ◽  
Jinyao Chen ◽  
Jia Lei ◽  
Zhao Ai ◽  
Feng Liu ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Biomarker Detection ◽  
High Demand ◽  
Coding Method

The existed multiplex biomarker detections are limited by the high demand for coding material and expensive detection equipment. This paper has proposed a convenient and precise coding method based on...

scholarly journals Metal Nanoparticle and Quantum Dot Tags for Signal Amplification in Electrochemical Immunosensors for Biomarker Detection

Chemosensors ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 85
Author(s):  
Anton Popov ◽  
Benediktas Brasiunas ◽  
Asta Kausaite-Minkstimiene ◽  
Almira Ramanaviciene
Keyword(s):  
Signal Amplification ◽  
Electrochemical Immunosensor ◽  
Metal Nanoparticle ◽  
Biomarker Detection ◽  
Analytical Instruments ◽  
Electrochemical Immunosensors ◽  
Highly Sensitive ◽  
Recent Developments ◽  
Electrochemical Signal ◽  
Detection Of Biomarkers

With the increasing importance of healthcare and clinical diagnosis, as well as the growing demand for highly sensitive analytical instruments, immunosensors have received considerable attention. In this review, electrochemical immunosensor signal amplification strategies using metal nanoparticles (MNPs) and quantum dots (Qdots) as tags are overviewed, focusing on recent developments in the ultrasensitive detection of biomarkers. MNPs and Qdots can be used separately or in combination with other nanostructures, while performing the function of nanocarriers, electroactive labels, or catalysts. Thus, different functions of MNPs and Qdots as well as recent advances in electrochemical signal amplification are discussed. Additionally, the methods most often used for antibody immobilization on nanoparticles, immunoassay formats, and electrochemical methods for indirect biomarker detection are overviewed.

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