Hyperspectral imaging method for noninvasive quantitative detection of vulvar lichen sclerosis: a pilot study

Author(s):  
YingJie Qu ◽  
Sui Feng ◽  
Ronald Xu
2021 ◽  
Vol 2 (43) ◽  
pp. 54-61
Author(s):  
Dmitriy A. Burynin ◽  
◽  
Aleksandr A. Smirnov

Portable spectroradiometers and hyperspectral cameras are increasingly being used to quickly assess the physiological state of plants. The operation of these devices is based on the registration of reflection or reflection and transmission spectra. (Research purpose) The research purpose is in analyzing the technical means and methods of non-invasive monitoring of the plant state based on the registration of the reflection spectra of leaves. (Materials and methods) The article presents a review of the work on the application of hyperspectral imaging methods. Authors classified and analyzed materials on spectroscopic radiometers and hyperspectral cameras, and outlined the prospects for implementation. Authors applied the methods of a systematic approach to the research problem. (Results and discussion) Hyperspectral imaging methods serve as an effective means of monitoring plants. It is possible to determine the pigment composition of plants, lack of nutrition, and detect biotic stress through hyperspectral imaging. The article presents methods of application of portable spectroradiometers and hyperspectral cameras. With the help of these devices it is possible to carry out measurements with high spectral resolution. The difficulty of accurately detecting the content of pigments in the leaves lies in the mutual overlap of the areas of light absorption by them. The main drawback of spectroradiometers is that they measure only at one point on a single sheet. The article presents the difficulties encountered in interpreting the results obtained by the hyperspectral camera. The background reflectivity of the soil, the geometry of the vegetation cover, and the uneven lighting can make errors in the measurements. (Conclusions) The article presents the disadvantages of the hyperspectral imaging method when using only the reflection spectrum. In order to increase the accuracy of the determination of pigments and stresses of various origins, it is necessary to develop a portable device that combines the methods of recording reflection and fluorescence.


2020 ◽  
Vol 1007 ◽  
pp. 105-110
Author(s):  
Xin Xin Wang ◽  
Cheng He ◽  
Pu Zhi Zhao ◽  
Yi Zheng ◽  
Shi Hao Jiang ◽  
...  

In this paper, a new ultrasonic phased array full focus imaging method based on oblique incidence is proposed to solve the problem of the non-destructive testing of the internal defects in the GIS (gas insulated switchgear) shell welds. By using wedge coupling, the measured weld is far away from the near-field range of the transducer, and the detection angle range can be increased by changing the propagation direction of the acoustic beam. Based on Snell's law, the propagation characteristics of the ultrasonic wave in the interface are studied. On the basis of the conventional ultrasonic array matrix and the full focus imaging algorithm, by introducing the energy attenuation calibration coefficient of the acoustic wave propagation through the wedge, the correction amplitude of the specific focus point p(x, z) is obtained, The non-destructive testing of weld defects of GIS shell in the spot is carried out, and the test results show that the qualitative and quantitative detection of the weld defects can be well realized by using this method.


2018 ◽  
Vol 90 (3) ◽  
pp. 493-506 ◽  
Author(s):  
Emeline Pouyet ◽  
Neda Rohani ◽  
Aggelos K. Katsaggelos ◽  
Oliver Cossairt ◽  
Marc Walton

AbstractVisible hyperspectral imaging (HSI) is a fast and non-invasive imaging method that has been adapted by the field of conservation science to study painted surfaces. By collecting reflectance spectra from a 2D surface, the resulting 3D hyperspectral data cube contains millions of recorded spectra. While processing such large amounts of spectra poses an analytical and computational challenge, it also opens new opportunities to apply powerful methods of multivariate analysis for data evaluation. With the intent of expanding current data treatment of hyperspectral datasets, an innovative approach for data reduction and visualization is presented in this article. It uses a statistical embedding method known as t-distributed stochastic neighbor embedding (t-SNE) to provide a non-linear representation of spectral features in a lower 2D space. The efficiency of the proposed method for painted surfaces from cultural heritage is established through the study of laboratory prepared paint mock-ups, and medieval French illuminated manuscript.


2015 ◽  
Vol 19 (5) ◽  
pp. 974-983 ◽  
Author(s):  
Mohammad Hadi Khoshtaghaza ◽  
Mostafa Khojastehnazhand ◽  
Barat Mojaradi ◽  
Mohammad Goodarzi ◽  
Wouter Saeys

2019 ◽  
Vol 16 (2) ◽  
pp. 143
Author(s):  
JR Lessy Eka Putri ◽  
Minarni Minarni ◽  
Feri Candra ◽  
Herman Herman

The hyperspectral imaging method has been widely and intensively used in agriculture to find out various problems that occur in plants. Image processing is very important step in an imaging method. This research aims to create Matlab based program to be used to analyze the leaf image of oil palm plants that has experienced water deficiency. Reflectance intensity values were extracted from the process. The hyperspectral imaging system consisted of a 650 nm diode laser, a spectrograph, monochrome CMOS camera, and Matlab image processing program. The samplesused were 8 month old Tenera variety of oil palm seedlings which were treated to simulate water deficiency in the form of variations in the volume of water, namely 0 mL (without watering), 1000 mL, 2000 mL, and 3000 mL (normal), 3 duplicates for each volume. The samples were given water volume of 1000 mL and 2000 mL for every 7 days in 21 days, while the sampleswith 3000 mL of water were watered every day. Image recording was done on the 21st day for detached leaves at the the bottom part.The results showed that the Matlab program was able to separate each row from 15 images, each of which had a pixel size of 1280 × 1024 and merge each of the same lines into 1024 images with a pixel size of 1280 × 15. The reflectance intensity values were then obtained. The results showed that higher levels of water deficiency in plants produce increasing reflectance intensity values.


2018 ◽  
Author(s):  
Yina Wang ◽  
Bin Yang ◽  
Siyu Feng ◽  
Veronica Pessino ◽  
Bo Huang

AbstractHyperspectral imaging is a powerful technique to simultaneously study multiple fluorophore labels with overlapping emissions. Here we present a computational hyperspectral imaging method, which uses the sample spatial fluorescence information as a reconstruction constraint. Our method addresses both the under-sampling issue of compressive hyperspectral imaging and the low throughput issue of scanning hyperspectral imaging. With simulated and experimental data, we have demonstrated the superior reconstruction precision of our method in two and three-color imaging. We have experimentally validated this method in differentiating cellular structures labeled with two red-colored fluorescent proteins, tdTomato and mCherry, which have highly overlapping emission spectra. Our method has the advantage of totally free wavelength choice and can also be combined with conventional filter-based sequential multi-color imaging to further expand the choices of probes.


2021 ◽  
Author(s):  
Kaytee Pokrzywinski ◽  
Cliff Morgan ◽  
Scott Bourne ◽  
Molly Reif ◽  
Kenneth Matheson ◽  
...  

To assist US Army Corps of Engineers resource managers in monitoring for cyanobacteria bloom events, a laboratory method using hyperspectral imaging has been developed. This method enables the rapid detection of cyanobacteria in large volumes and has the potential to be transitioned to aerial platforms for field deployment. Prior to field data collection, validation of the technology in the laboratory using monocultures was needed. This report describes the development of the detection method using hyperspectral imaging and the stability/reliability of these signatures for identification purposes. Hyperspectral signatures of different cyanobacteria were compared to evaluate spectral deviations between genera to assess the feasibility of using this imaging method in the field. Algorithms were then developed to spectrally deconvolute mixtures of cyanobacteria to determine relative abundances of each species. Last, laboratory cultures of Microcystis aeruginosa and Anabaena sp. were subjected to varying macro (nitrate and phosphate) and micro-nutrient (iron and magnesium) stressors to establish the stability of signatures within each species. Based on the findings, hyperspectral imaging can be a valuable tool for the detection and monitoring of cyanobacteria. However, it should be used with caution and only during stages of active growth for accurate identification and limited interference owing to stress.


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