Two-step preparation and properties of polyimide / zirconium composite free-supporting membrane

2021 ◽  
Author(s):  
Ren-Chen Liu ◽  
JING LU
Keyword(s):  
Supporting Membrane

Gold labeling of teichoic acid on adjacent surfaces of staphylococcus aureus

1990 ◽  
Vol 48 (3) ◽  
pp. 578-579
Author(s):  
Margaret Hukee
Keyword(s):  
Staphylococcus Aureus ◽  
Teichoic Acid ◽  
Protein Labeling ◽  
Minimal Amount ◽  
Section Thickness ◽  
Double Labeling ◽  
Parallel Surfaces ◽  
Supporting Membrane

Gold labeling of two antigens (double labeling) is often done on two section surfaces separated by section thickness. Whether labeling is done on both sides of the same section or on two parallel surfaces separated by section thickness (PSSST), comparable results are dependent on an equal number of epitopes being exposed at each surface. We propose a method to study protein labeling within the same field of proteins, by examining two directly adjacent surfaces that were split during sectioning. The number of labeling sites on adjacent surfaces (AS) were compared to sites on PSSST surfaces in individual bacteria.Since each bacteria needed to be recognizable in all three section surfaces, one-hole grids were used for labeling. One-hole grids require a supporting membrane and excessive handling during labeling often ruptures the membrane. To minimize handling, a labeling chamber was designed that is inexpensive, disposable, minimizes contamination, and uses a minimal amount of solution.

scholarly journals Yeast vacuolar HOPS, regulated by its kinase, exploits affinities for acidic lipids and Rab:GTP for membrane binding and to catalyze tethering and fusion

2015 ◽  
Vol 26 (2) ◽  
pp. 305-315 ◽  
Author(s):  
Amy Orr ◽  
William Wickner ◽  
Scott F. Rusin ◽  
Arminja N. Kettenbach ◽  
Michael Zick
Keyword(s):  
Protein Sorting ◽  
Rab Gtpase ◽  
Attachment Protein ◽  
Functional Relationships ◽  
Sensitive Factor ◽  
Protein Receptors ◽  
Membrane Tethering ◽  
Rab Effector ◽  
Supporting Membrane ◽  
Acidic Lipids

Fusion of yeast vacuoles requires the Rab GTPase Ypt7p, four SNAREs (soluble N-ethylmaleimide–sensitive factor attachment protein receptors), the SNARE disassembly chaperones Sec17p/Sec18p, vacuolar lipids, and the Rab-effector complex HOPS (homotypic fusion and vacuole protein sorting). Two HOPS subunits have direct affinity for Ypt7p. Although vacuolar fusion has been reconstituted with purified components, the functional relationships between individual lipids and Ypt7p:GTP have remained unclear. We now report that acidic lipids function with Ypt7p as coreceptors for HOPS, supporting membrane tethering and fusion. After phosphorylation by the vacuolar kinase Yck3p, phospho-HOPS needs both Ypt7p:GTP and acidic lipids to support fusion.

scholarly journals Construction of Sandwich-like cell sheets with natural electrospun gelatin/chitosan nanofibrous delivered plasmid VEGF for accelerated wound healing

2020 ◽  
Author(s):  
Yanxia Zhu ◽  
Yuqi Liao ◽  
Yuanyuan Zhang ◽  
Xinyi Wang ◽  
Jianhao Zhang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Physical Properties ◽  
Skin Substitute ◽  
Cell Sheets ◽  
Effective Technique ◽  
Support Cell ◽  
And Migration ◽  
Electrospun Gelatin ◽  
Supporting Membrane ◽  
Proliferation And Migration

Abstract Background Development of natural biodegradable electrospun nanofibrous with appropriate physical properties and biocompatibility is highly desirable to support multi-layer cell sheets construction for wound healing. Results We developed a series of electrospun gelatin/chitosan nanofibrous with different gelatin/chitosan ratios and controlled pore sizes, and impregnated plasmid VEGF into membrane, which as supporting membrane to construct sandwich-like adipose-derived stem cells (ADSCs) cell sheets with a simple and effective technique for accelerated wound healing. We found that the physical properties of the electrospun nanofibrous including water retention, stiffness, strength, elasticity and degradation could be tailored by changing the proportion of gelatin/chitosan. We further observed that the optimized electrospun nanofibrous with the optimal ratio of gelatin to chitosan (7:3) which were soft and elastic could most effectively support cell adhesion, proliferation and migration into the whole nanofibrous membranes. Nanofibrous delivered plasmid VEGF facilitating multi-layer ADSC cell sheets formation and promoting regeneration of cutaneous tissues within two weeks. Conclusions Such natural biodegradable and biocompatible electrospun gelatin/chitosan nanofibrous with plasmid have the potential to become fully cellularized and support sandwich-like ADSC cell sheets formation, which will make it suitable for widespread applications such as skin substitute or wound dressing.

Effects of halothane and supporting membrane lipids on the activity of acetylcholinesterase

1998 ◽  
Vol 100-101 ◽  
pp. 447-450 ◽  
Author(s):  
I. Tsukamoto ◽  
S. Yokono ◽  
H. Komatsu ◽  
K. Ogli
Keyword(s):  
Membrane Lipids ◽  
Supporting Membrane

Immobilization of laccase on functionalized multiwalled carbon nanotube membranes and application for dye decolorization

RSC Advances ◽  
2016 ◽  
Vol 6 (115) ◽  
pp. 114690-114697 ◽  
Author(s):  
Abdelmageed M. Othman ◽  
Elena González-Domínguez ◽  
Ángeles Sanromán ◽  
Miguel Correa-Duarte ◽  
Diego Moldes
Keyword(s):  
Carbon Nanotubes ◽  
Carbon Nanotube ◽  
Dye Decolorization ◽  
Myceliophthora Thermophila ◽  
Multiwalled Carbon ◽  
Continuous Processes ◽  
Nanotube Membranes ◽  
Carbon Nanotube Membranes ◽  
Functionalized Multiwalled Carbon Nanotubes ◽  
Supporting Membrane

Myceliophthora thermophilalaccase was covalently immobilized on functionalized multiwalled carbon nanotubes (MWNT) arranged over a supporting membrane to obtain a permeable bio-barrier that could be applied in multibatch or continuous processes.

A polymeric ionic liquid functionalized temperature-responsive composite membrane with tunable responsive behavior

2015 ◽  
Vol 3 (15) ◽  
pp. 7919-7928 ◽  
Author(s):  
Ying Tang ◽  
Beibei Tang ◽  
Peiyi Wu
Keyword(s):  
Ionic Liquid ◽  
Composite Membrane ◽  
Polymeric Ionic Liquid ◽  
Temperature Responsive ◽  
Responsive Behavior ◽  
Supporting Membrane

A temperature-responsive composite membrane with tunable responsive behavior was obtained by chemically grafting a PIL functionalized temperature-responsive copolymer onto the BPPO supporting membrane.

Reconstitution of bacteriorhodopsin with cationic poly(dimethylaminoethyl acrylate)-block-poly(methylacrylate) for bio-hybrid materials

RSC Advances ◽  
2016 ◽  
Vol 6 (10) ◽  
pp. 7955-7960
Author(s):  
Weiwei He ◽  
Jiaqi Ma ◽  
Xiaowen Shu ◽  
Jun Qian ◽  
Daoben Hua
Keyword(s):  
Membrane Proteins ◽  
Hybrid Materials ◽  
Amphiphilic Copolymer ◽  
Supporting Membrane

A kind of cationic amphiphilic copolymer was synthesized for supporting membrane proteins to prepare bio-hybrid materials.

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