High speed volumetric imaging for brain connectomics

2021 ◽  
Author(s):  
Shi-Wei Chu
Keyword(s):  
High Speed ◽  
Volumetric Imaging ◽  
Brain Connectomics

scholarly journals High-speed volumetric imaging of neuronal activity in freely moving rodents

2018 ◽  
Vol 15 (6) ◽  
pp. 429-432 ◽  
Author(s):  
Oliver Skocek ◽  
Tobias Nöbauer ◽  
Lukas Weilguny ◽  
Francisca Martínez Traub ◽  
Chuying Naomi Xia ◽  
...  
Keyword(s):  
Neuronal Activity ◽  
High Speed ◽  
Volumetric Imaging ◽  
Freely Moving

scholarly journals High-speed Fly-Scan Volumetric Imaging

2018 ◽  
Vol 24 (S1) ◽  
pp. 498-499 ◽  
Author(s):  
Doga Gursoy
Keyword(s):  
High Speed ◽  
Volumetric Imaging

In-vivo volumetric imaging of the cellular structure of healthy and pathological human cornea with high-speed UHR-OCT (Conference Presentation)

2018 ◽  
Author(s):  
Zohreh Hosseinaee ◽  
Bingyao Tan ◽  
Kirsten Carter ◽  
Denise Hileeto ◽  
Luigina Sorbara ◽  
...  
Keyword(s):  
Cellular Structure ◽  
High Speed ◽  
Human Cornea ◽  
Volumetric Imaging

scholarly journals Distal convoluted tubule sexual dimorphism revealed by advanced 3D imaging

2020 ◽  
Vol 319 (5) ◽  
pp. F754-F764
Author(s):  
Ebrahim Tahaei ◽  
Richard Coleman ◽  
Turgay Saritas ◽  
David H. Ellison ◽  
Paul A. Welling
Keyword(s):  
Sexual Dimorphism ◽  
High Speed ◽  
Distal Convoluted Tubule ◽  
Volumetric Imaging ◽  
Female Mice ◽  
Cellular Density ◽  
The Face ◽  
Antibody Labeling ◽  
Male Subjects ◽  
Insight Into

The thiazide-sensitive Na+-Cl− cotransporter (NCC) is more abundant in kidneys of female subjects than of male subjects. Because morphological remodeling of the distal convoluted tubule (DCT) is dependent on NCC activity, it has been generally assumed that there is a corresponding sexual dimorphism in the structure of the DCT, leading to a larger female DCT. Until now, this has never been directly examined. Here, optical clearing techniques were combined with antibody labeling of DCT segment markers, state-of-the-art high-speed volumetric imaging, and analysis tools to visualize and quantify DCT morphology in male and female mice and study the DCT remodeling response to furosemide. We found an unexpected sex difference in the structure of the DCT. Compared with the male mice, female mice had a shorter DCT, a higher cellular density of NCC, and a greater capacity to elongate in response to loop diuretics. Our study revealed a sexual dimorphism of the DCT. Female mice expressed a greater density of NCC transporters in a shorter structure to protect Na+ balance in the face of greater basal distal Na+ delivery yet have a larger reserve and structural remodeling capacity to adapt to unique physiological stresses. These observations provide insight into mechanisms that may drive sex differences in the therapeutic responses to diuretics.

Three Dimensional Imaging of LIGA-Made Microcomponents

2004 ◽  
Vol 126 (4) ◽  
pp. 813-821 ◽  
Author(s):  
Douglas Chinn ◽  
Peter Ostendorp ◽  
Mike Haugh ◽  
Russell Kershmann ◽  
Thomas Kurfess ◽  
...  
Keyword(s):  
Computer Aided Design ◽  
High Speed ◽  
Three Dimensional ◽  
Point Clouds ◽  
Three Dimensions ◽  
Cad Model ◽  
Volumetric Imaging ◽  
Data Registration ◽  
Liga Technology ◽  
Coordinate Metrology

Nickel and nickel-alloy microparts sized on the order of 5–1000 microns have been imaged in three dimensions using a new microscopic technique, Digital Volumetric Imaging (DVI). The gears were fabricated using Sandia National Laboratories’ LIGA technology (lithography, molding, and electroplating). The images were taken on a microscope built by Resolution Sciences Corporation by slicing the gear into one-micron thin slices, photographing each slice, and then reconstructing the image with software. The images were matched to the original CAD (computer aided design) model, allowing LIGA designers, for the first time, to see visually how much deviation from the design is induced by the manufacturing process. Calibration was done by imaging brass ball bearings and matching them to the CAD model of a sphere. A major advantage of DVI over scanning techniques is that internal defects can be imaged to very high resolution. In order to perform the metrology operations on the microcomponents, high-speed and high-precision algorithms are developed for coordinate metrology. The algorithms are based on a least-squares approach to data registration the {X,Y,Z} point clouds generated from the component surface onto a target geometry defined in a CAD model. Both primitive geometric element analyses as well as an overall comparison of the part geometry are discussed. Initial results of the micromeasurements are presented in the paper.

scholarly journals A Multifocal Multiphoton Volumetric Imaging Technique for High Speed Time-Resolved FRET Imaging in vivo

2016 ◽  
Vol 110 (3) ◽  
pp. 165a ◽  
Author(s):  
Simon P. Poland ◽  
James A. Levitt ◽  
Nikola Krstajić ◽  
Ahmet Erdogen ◽  
Richard J. Walker ◽  
...  
Keyword(s):  
High Speed ◽  
Imaging Technique ◽  
Time Resolved ◽  
Volumetric Imaging

scholarly journals Three-axis digital holographic microscopy for high speed volumetric imaging

2014 ◽  
Vol 22 (11) ◽  
pp. 13710 ◽  
Author(s):  
F. Saglimbeni ◽  
S. Bianchi ◽  
A. Lepore ◽  
R. Di Leonardo
Keyword(s):  
High Speed ◽  
Digital Holographic Microscopy ◽  
Volumetric Imaging ◽  
Holographic Microscopy

scholarly journals Dual-color volumetric imaging of neural activity of cortical columns

2018 ◽  
Author(s):  
Shuting Han ◽  
Weijian Yang ◽  
Rafael Yuste
Keyword(s):  
Neural Activity ◽  
High Speed ◽  
Neural Circuits ◽  
Emergent Properties ◽  
Spatiotemporal Resolution ◽  
Population Activity ◽  
Volumetric Imaging ◽  
Two Photon ◽  
Dual Color

To capture the emergent properties of neural circuits, high-speed volumetric imaging of neural activity at cellular resolution is desirable. But while conventional two-photon calcium imaging is a powerful tool to study population activity in vivo, it is restrained to two-dimensional planes. Expanding it to 3D while maintaining high spatiotemporal resolution appears necessary. Here, we developed a two-photon microscope with dual-color laser excitation that can image neural activity in a 3D volume. We imaged the neuronal activity of primary visual cortex from awake mice, spanning from L2 to L5 with 10 planes, at a rate of 10 vol/sec, and demonstrated volumetric imaging of L1 long-range PFC projections and L2/3 somatas. Using this method, we map visually-evoked neuronal ensembles in 3D, finding a lack of columnar structure in orientation responses and revealing functional correlations between cortical layers which differ from trial to trial and are missed in sequential imaging. We also reveal functional interactions between presynaptic L1 axons and postsynaptic L2/3 neurons. Volumetric two-photon imaging appears an ideal method for functional connectomics of neural circuits.

scholarly journals Single-Shot Optical Projection tomography for high-speed volumetric imaging

2021 ◽  
Author(s):  
Connor James Darling ◽  
Samuel P.X. Davis ◽  
Sunil Kumar ◽  
Paul M.W. French ◽  
James A McGinty
Keyword(s):  
High Speed ◽  
Low Cost ◽  
Single Shot ◽  
Optical Projection Tomography ◽  
Data Set ◽  
Volumetric Imaging ◽  
Optical Projection ◽  
Volumetric Reconstruction ◽  
Projection Images

We present a single-shot adaptation of Optical Projection Tomography (OPT) for high-speed volumetric snapshot imaging of dynamic mesoscopic samples. Conventional OPT has been applied to in vivo imaging of animal models such as D. rerio but the sequential acquisition of projection images required for volumetric reconstruction typically requires samples to be immobilised during the acquisition of an OPT data set. We present a proof-of-principle system capable of single-shot imaging of a 1 mm diameter volume, demonstrating camera-limited rates of up to 62.5 volumes/second, which we have applied to 3D imaging of a freely-swimming zebrafish embryo. This is achieved by recording 8 projection views simultaneously on 4 low-cost CMOS cameras. With no stage required to rotate the sample, this single-shot OPT system can be implemented with a component cost of under 5,000GBP. The system design can be adapted to different sized fields of view and may be applied to a broad range of dynamic samples, including fluid dynamics.

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