Correlation analysis of human iPSC-derived neuronal networks using holographic single cell and full field stimulation

2021 ◽  
Author(s):  
Felix Schmieder ◽  
Rouhollah Habibey ◽  
Volker Busskamp ◽  
Lars Büttner ◽  
Jürgen W. Czarske
Keyword(s):  
Correlation Analysis ◽  
Single Cell ◽  
Neuronal Networks ◽  
Field Stimulation ◽  
Full Field ◽  
Human Ipsc

Analysis of human iPSC-derived neuronal networks (hiPSCNN) using holographic single cell and full field optogenetic stimulation

2021 ◽  
Author(s):  
Felix Schmieder ◽  
Rouhollah Habibey ◽  
Volker Busskamp ◽  
Lars Büttner ◽  
Jürgen W. Czarske
Keyword(s):  
Single Cell ◽  
Neuronal Networks ◽  
Full Field ◽  
Optogenetic Stimulation ◽  
Human Ipsc

scholarly journals Threshold vision under full-field stimulation: Revisiting the minimum number of quanta necessary to evoke a visual sensation

2021 ◽  
Vol 180 ◽  
pp. 1-10
Author(s):  
Ashim Dey ◽  
Andrew J. Zele ◽  
Beatrix Feigl ◽  
Prakash Adhikari
Keyword(s):  
Visual Sensation ◽  
Field Stimulation ◽  
Full Field ◽  
Minimum Number

Active pixel sensor array for high spatio-temporal resolution electrophysiological recordings from single cell to large scale neuronal networks

Lab on a Chip ◽  
2009 ◽  
Vol 9 (18) ◽  
pp. 2644 ◽  
Author(s):  
Luca Berdondini ◽  
Kilian Imfeld ◽  
Alessandro Maccione ◽  
Mariateresa Tedesco ◽  
Simon Neukom ◽  
...  
Keyword(s):  
Single Cell ◽  
Temporal Resolution ◽  
Large Scale ◽  
Neuronal Networks ◽  
Sensor Array ◽  
Active Pixel Sensor ◽  
Electrophysiological Recordings ◽  
Active Pixel ◽  
Spatio Temporal

scholarly journals Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Ayshwarya Subramanian ◽  
Eriene-Heidi Sidhom ◽  
Maheswarareddy Emani ◽  
Katherine Vernon ◽  
Nareh Sahakian ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Human Kidney ◽  
Mouse Kidney ◽  
Target Cells ◽  
Rna Seq ◽  
Kidney Capsule ◽  
Time Points ◽  
Human Ipsc ◽  
Kidney Organoids

AbstractHuman iPSC-derived kidney organoids have the potential to revolutionize discovery, but assessing their consistency and reproducibility across iPSC lines, and reducing the generation of off-target cells remain an open challenge. Here, we profile four human iPSC lines for a total of 450,118 single cells to show how organoid composition and development are comparable to human fetal and adult kidneys. Although cell classes are largely reproducible across time points, protocols, and replicates, we detect variability in cell proportions between different iPSC lines, largely due to off-target cells. To address this, we analyze organoids transplanted under the mouse kidney capsule and find diminished off-target cells. Our work shows how single cell RNA-seq (scRNA-seq) can score organoids for reproducibility, faithfulness and quality, that kidney organoids derived from different iPSC lines are comparable surrogates for human kidney, and that transplantation enhances their formation by diminishing off-target cells.

scholarly journals Epileptiform Activities in Cultured Human IPSC-Derived Neuronal Networks

2018 ◽  
Vol 114 (3) ◽  
pp. 672a
Author(s):  
Ikuro Suzuki ◽  
Aoi Odawara ◽  
Naoki Matsuda ◽  
Yuto Ishibashi ◽  
Remi Yokoi
Keyword(s):  
Neuronal Networks ◽  
Human Ipsc

scholarly journals Label-free characterization of vitrification-induced morphology changes in single-cell embryos with full-field optical coherence tomography

2015 ◽  
Vol 20 (9) ◽  
pp. 096004 ◽  
Author(s):  
Livia Zarnescu ◽  
Michael C. Leung ◽  
Michael Abeyta ◽  
Helge Sudkamp ◽  
Thomas Baer ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Single Cell ◽  
Optical Coherence ◽  
Label Free ◽  
Full Field ◽  
Morphology Changes

Self-Aligned Functionalization Approach to Order Neuronal Networks at the Single-Cell Level

Langmuir ◽  
2018 ◽  
Vol 34 (22) ◽  
pp. 6612-6620 ◽  
Author(s):  
Adrien Casanova ◽  
Marie-Charline Blatche ◽  
Cécile A. Ferre ◽  
Hélène Martin ◽  
Daniel Gonzalez-Dunia ◽  
...  
Keyword(s):  
Single Cell ◽  
Neuronal Networks ◽  
Single Cell Level ◽  
Cell Level

Investigation of in vitro human iPSC-derived neuronal networks using holographic stimulation (Conference Presentation)

2020 ◽  
Author(s):  
Felix Schmieder ◽  
Rouhollah Habibey ◽  
Volker Busskamp ◽  
Lars Büttner ◽  
Jürgen W. Czarske
Keyword(s):  
Neuronal Networks ◽  
Human Ipsc
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