Matrix approach of adaptive optics for the imaging of scattering media

Author(s):  
Ulysse Najar ◽  
Paul Balondrade ◽  
Victor Barolle ◽  
Laura A. Cobus ◽  
Claude A. Boccara ◽  
...  
2017 ◽  
Vol 25 (9) ◽  
pp. 10368 ◽  
Author(s):  
Xiaodong Tao ◽  
Tuwin Lam ◽  
Bingzhao Zhu ◽  
Qinggele Li ◽  
Marc R. Reinig ◽  
...  

2018 ◽  
Author(s):  
Jeffrey A. Mulligan ◽  
Xinzeng Feng ◽  
Steven G. Adie

AbstractCellular traction forces (CTFs) play an integral role in both physiological processes and disease, and are a topic of interest in mechanobiology. Traction force microscopy (TFM) is a family of methods used to quantify CTFs in a variety of settings. State-of-the-art 3D TFM methods typically rely on confocal fluorescence microscopy, which can impose limitations on acquisition speed, volumetric coverage, and temporal sampling or coverage. In this report, we present the first quantitative implementation of a new TFM technique: traction force optical coherence microscopy (TF-OCM). TF-OCM leverages the capabilities of optical coherence microscopy and computational adaptive optics (CAO) to enable the quantitative reconstruction of 3D CTFs in scattering media with minute-scale temporal resolution. We applied TF-OCM to quantify CTFs exerted by isolated NIH-3T3 fibroblasts embedded in Matrigel, with five-minute temporal sampling, using images which spanned a 500×500×500 μm3 field-of-view. Due to the reliance of TF-OCM on computational imaging methods, we have provided extensive discussion of the underlying equations, assumptions, and failure modes of these methods. TF-OCM has the potential to advance studies of biomechanical behavior in scattering media, and may be especially well-suited to the study of cell collectives such as spheroids, a prevalent model in mechanobiology research.


2020 ◽  
Vol 10 (2) ◽  
Author(s):  
William Lambert ◽  
Laura A. Cobus ◽  
Mathieu Couade ◽  
Mathias Fink ◽  
Alexandre Aubry

2020 ◽  
Vol 6 (30) ◽  
pp. eaay7170 ◽  
Author(s):  
Amaury Badon ◽  
Victor Barolle ◽  
Kristina Irsch ◽  
A. Claude Boccara ◽  
Mathias Fink ◽  
...  

In optical imaging, light propagation is affected by the inhomogeneities of the medium. Sample-induced aberrations and multiple scattering can strongly degrade the image resolution and contrast. On the basis of a dynamic correction of the incident and/or reflected wavefronts, adaptive optics has been used to compensate for those aberrations. However, it only applies to spatially invariant aberrations or to thin aberrating layers. Here, we propose a global and noninvasive approach based on the distortion matrix concept. This matrix basically connects any focusing point of the image with the distorted part of its wavefront in reflection. A singular value decomposition of the distortion matrix allows to correct for high-order aberrations and forward multiple scattering over multiple isoplanatic modes. Proof-of-concept experiments are performed through biological tissues including a turbid cornea. We demonstrate a Strehl ratio enhancement up to 2500 and recover a diffraction-limited resolution until a depth of 10 scattering mean free paths.


2021 ◽  
Author(s):  
Elsa Giraudat ◽  
Arnaud Burtin ◽  
Alexandre Aubry

<p>Volcanoes are among the most challenging media for seismic imaging given their highly localized and abrupt variations in physical parameters, extreme landforms, fractures, and the presence of magma and other fluids. Because of this high level of heterogeneity and the resulting difficulty to access the wave velocity distribution in the medium, reflection seismic imaging of volcanoes usually suffers from a loss of resolution and contrast. Here, we present a passive seismic imaging technique applied to the case of La Soufrière of Guadeloupe volcano. Inspired by previous works in optics (Badon <em>et al</em>., 2020), in acoustics (Lambert <em>et al</em>., 2020), and recently introduced in seismology (Touma <em>et al</em>., 2020), this technique relies on a matrix approach of passive reflection imaging, which requires only a rough approximation about the medium background velocity. This makes it robust even applied to extreme environments as volcanoes or fault zones. In this approach, the Green’s functions between an array of 76 geophones placed at the surface of the volcano are retrieved by cross-correlation of ambient seismic noise. This set of 2850 inter-element impulse responses forms a reflection matrix. Focusing operations are applied to this reflection matrix at emission and reception to project it in–depth. The focusing process allows to extract body wave components from seismic noise and thus, to retrieve information about reflectivity of in-depth structures. However, at this point, reflectivity images of the subsurface still suffer from phase distortions induced by long-range variations of the seismic velocity. This results in blurred images and hinders appropriate imaging. To overcome these issues, a novel operator is introduced: the distortion matrix. This operator is derived from the focused reflection matrix and connects any point in the medium with the distortion that a wavefront emitted from that point would experience due to heterogeneity. A time-reversal analysis of the distortion matrix allows to retrieve aberrations phase laws and hence to compensate for phase distortions. This correction enables to recover 3D-images of the volcano’s subsurface for the first 10km below the summit with optimized contrast and with an increased resolution. Interestingly, the restored resolution is even at least one half below the diffraction limit imposed by the geophone array angular aperture at the surface. The obtained gain in resolution and contrast allows to unveil internal structures of La Soufrière as hypothetical volcanic vents, magma reservoirs and lateral drainage conduits.</p><p><strong>References</strong></p><p>[Badon <em>et al</em>., 2020] Badon, A., Barolle, V., Irsch, K., Boccara, A. C., Fink, M., and Aubry, A. (2020). Distortion matrix concept for deep optical imaging in scattering media. <em>Science Advances,</em> 6(30).</p><p>[Lambert <em>et al.</em>, 2020] Lambert, W., Cobus, L. A., Frappart, T., Fink, M., and Aubry, A. (2020). Distortion matrix approach for ultrasound imaging of random scattering media. <em>Proceedings of the National Academy of Sciences,</em> 117(26):14645-14656.</p><p>[Touma<em> et al.</em>, 2020] Touma, R., Blondel, T., Derode, A., Campillo, M., & Aubry, A. (2020). A Distortion Matrix Framework for High-Resolution Passive Seismic 3D Imaging: Application to the San Jacinto Fault Zone, California.<em> arXiv preprint arXiv</em>:2008.01608.</p>


2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jonas Kanngiesser ◽  
Maik Rahlves ◽  
Bernhard Roth

Abstract Spectral domain optical coherence tomography (SD-OCT) is a highly versatile method which allows for three dimensional optical imaging in scattering media. A number of recent publications demonstrated the technique to benefit from structured illumination and beam shaping approaches, e.g. to enhance the signal-to-noise ratio or the penetration depth with samples such as biological tissue. We present a compact and easy to implement design for independent wavefront manipulation and beam shaping at the reference and sample arm of the interferometric OCT device. The design requires a single spatial light modulator and can be integrated to existing free space SD-OCT systems by modifying the source arm only. We provide analytical and numerical discussion of the presented design as well as experimental data confirming the theoretical analysis. The system is highly versatile and lends itself for applications where independent phase or wavefront control is required. We demonstrate the system to be used for wavefront sensorless adaptive optics as well as for iterative optical wavefront shaping for OCT signal enhancement in strongly scattering media.


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