Optical property revealing vascular network and pathological lesion in human brain tissues with as-PSOCT

2021 ◽  
Author(s):  
Hui Wang ◽  
Robert J. Jones ◽  
Divya Varadarajan ◽  
Caroline Magnain ◽  
Anastasia Yendiki ◽  
...  
Keyword(s):  
Optical Property ◽  
Human Brain ◽  
Vascular Network ◽  
Pathological Lesion ◽  
Brain Tissues

Optical properties of selected native and coagulated human brain tissues in vitro in the visible and near infrared spectral range

2002 ◽  
Vol 47 (12) ◽  
pp. 2059-2073 ◽  
Author(s):  
A N Yaroslavsky ◽  
P C Schulze ◽  
I V Yaroslavsky ◽  
R Schober ◽  
F Ulrich ◽  
...  
Keyword(s):  
Optical Properties ◽  
Human Brain ◽  
Spectral Range ◽  
Near Infrared ◽  
Infrared Spectral Range ◽  
Infrared Spectral ◽  
Brain Tissues

Determination of metal elements concentrations in human brain tissues using PIXE and EDX methods

2018 ◽  
Vol 318 (3) ◽  
pp. 2313-2319
Author(s):  
Ján Pánik ◽  
Martin Kopáni ◽  
Jakub Zeman ◽  
Miroslav Ješkovský ◽  
Jakub Kaizer ◽  
...  
Keyword(s):  
Human Brain ◽  
Metal Elements ◽  
Brain Tissues

Double-overdamped-oscillator model of terahertz complex dielectric permittivity of human brain tissues

2021 ◽  
Author(s):  
Guzel R. Musina ◽  
Nikita V. Chernomyrdin ◽  
Irina N. Dolganova ◽  
Vladimir N. Kurlov ◽  
Pavel V. Nikitin ◽  
...  
Keyword(s):  
Dielectric Permittivity ◽  
Human Brain ◽  
Oscillator Model ◽  
Complex Dielectric Permittivity ◽  
Brain Tissues

scholarly journals From fix to fit into the autoptic human brains

2018 ◽  
Author(s):  
Beatrice Paradiso ◽  
Michele Simonato ◽  
Gaetano Thiene ◽  
Anna Lavezzi
Keyword(s):  
Human Brain ◽  
Rna Extraction ◽  
Sudden Infant Death ◽  
Adult Brain ◽  
Immunofluorescent Staining ◽  
Nuclear Antigen ◽  
Sudden Infant ◽  
Postmortem Human Brain ◽  
Modified Method ◽  
Brain Tissues

Formalin-fixed, paraffinembedded (FFPE) human brain tissues are very often stored in formalin for long time. Formalin fixation reduces immunostaining, and the DNA/RNA extraction from FFPE brain tissue becomes suboptimal. At present, there are different protocols of fixation and several procedures and kits to extract DNA/RNA from paraffin embedding tissue, but a gold standard protocol remains distant. In this study, we analyzed four types of fixation systems and compared histo and immuno-staining. Based on our results, we propose a modified method of combined fixation in formalin and formic acid for the autoptic adult brain to obtain easy, fast, safe and efficient immunolabelling of long-stored FFPE tissue. In particular, we have achieved an improved preservation of cellular morphology and obtained success in postmortem immunostaining for NeuN. This nuclear antigen is an important marker for mapping neurons, for example, to evaluate the histopathology of temporal lobe epilepsy or to draw the topography of cardiorespiratory brainstem nuclei in sudden infant death syndrome (SIDS). However, NeuN staining is frequently faint or lost in postmortem human brain tissues. In addition, we attained Fluoro Jade C staining, a marker of neurodegeneration, and immunofluorescent staining for stem cell antigens in the postnatal human brain, utilizing custom fit fixation procedures.

Convergent lines of evidence support NOTCH4 as a schizophrenia risk gene

2020 ◽  
pp. jmedgenet-2020-106830
Author(s):  
Yan Zhang ◽  
Shiwu Li ◽  
Xiaoyan Li ◽  
Yongfeng Yang ◽  
Wenqiang Li ◽  
...  
Keyword(s):  
Human Brain ◽  
Large Scale ◽  
Meta Analysis ◽  
Eqtl Analysis ◽  
Risk Gene ◽  
And Migration ◽  
Eqtl Data ◽  
Lines Of Evidence ◽  
Brain Tissues

The association between NOTCH4 and schizophrenia has been repeatedly reported. However, the results from different genetic studies are inconsistent, and the role of NOTCH4 in schizophrenia pathogenesis remains unknown. Here, we provide convergent lines of evidence that support NOTCH4 as a schizophrenia risk gene. We first performed a meta-analysis and found that a genetic variant (rs2071287) in NOTCH4 was significantly associated with schizophrenia (a total of 125 848 subjects, p=8.31×10−17), with the same risk allele across all tested samples. Expression quantitative trait loci (eQTL) analysis showed that rs2071287 was significantly associated with NOTCH4 expression (p=1.08×10−14) in human brain tissues, suggesting that rs2071287 may confer schizophrenia risk through regulating NOTCH4 expression. Sherlock integrative analysis using a large-scale schizophrenia GWAS and eQTL data from human brain tissues further revealed that NOTCH4 was significantly associated with schizophrenia (p=4.03×10−7 in CMC dataset and p=3.06×10−6 in xQTL dataset), implying that genetic variants confer schizophrenia risk through modulating NOTCH4 expression. Consistently, we found that NOTCH4 was significantly downregulated in brains of schizophrenia patients compared with controls (p=2.53×10−3), further suggesting that dysregulation of NOTCH4 may have a role in schizophrenia. Finally, we showed that NOTCH4 regulates proliferation, self-renewal, differentiation and migration of neural stem cells, suggesting that NOTCH4 may confer schizophrenia risk through affecting neurodevelopment. Our study provides convergent lines of evidence that support the involvement of NOTCH4 in schizophrenia. In addition, our study also elucidates a possible mechanism for the role of NOTCH4 in schizophrenia pathogenesis.

P2-108: Evaluation of a radiolabelled butyrylcholinesterase ligand, N-methyl-4-piperdinyl-4-[18F]-fluorobenzoate, in human brain tissues

2013 ◽  
Vol 9 ◽  
pp. P385-P385
Author(s):  
Courtney Jollymore ◽  
Ian Macdonald ◽  
Ian Pottie ◽  
Earl Martin ◽  
Sultan Darvesh
Keyword(s):  
Human Brain ◽  
Brain Tissues
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