Fluorescence lifetime analysis and measurement in sub-100 nm gold nanoapertures

2021 ◽  
Author(s):  
Changhun Lee ◽  
Wonsang Hwang ◽  
Dugyoung Kim ◽  
Donghyun Kim
Keyword(s):  
Fluorescence Lifetime ◽  
Lifetime Analysis

scholarly journals Analysis of Fluorescence Decay Kinetics of Indocyanine Green Monomers and Aggregates in Brain Tumor Model In Vivo

Nanomaterials ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3185
Author(s):  
Dina Farrakhova ◽  
Igor Romanishkin ◽  
Yuliya Maklygina ◽  
Lina Bezdetnaya ◽  
Victor Loschenov
Keyword(s):  
Brain Tumor ◽  
Indocyanine Green ◽  
Fluorescence Lifetime ◽  
Tumor Model ◽  
Fluorescence Decay ◽  
Decay Kinetics ◽  
Lifetime Analysis ◽  
Fluorescence Decay Kinetics ◽  
Brain Tumor Model

Spectroscopic approach with fluorescence time resolution allows one to determine the state of a brain tumor and its microenvironment via changes in the fluorescent dye’s fluorescence lifetime. Indocyanine green (ICG) is an acknowledged infra-red fluorescent dye that self-assembles into stable aggregate forms (ICG NPs). ICG NPs aggregates have a tendency to accumulate in the tumor with a maximum accumulation at 24 h after systemic administration, enabling extended intraoperative diagnostic. Fluorescence lifetime analysis of ICG and ICG NPs demonstrates different values for ICG monomers and H-aggregates, indicating promising suitability for fluorescent diagnostics of brain tumors due to their affinity to tumor cells and stability in biological tissue.

scholarly journals In Vivo Time Domain Optical Imaging of Renal Ischemia-Reperfusion Injury: Discrimination Based on Fluorescence Lifetime

2007 ◽  
Vol 6 (5) ◽  
pp. 7290.2007.00030 ◽  
Author(s):  
Abedelnasser Abulrob ◽  
Eric Brunette ◽  
Jacqueline Slinn ◽  
Ewa Baumann ◽  
Danica Stanimirovic
Keyword(s):  
Optical Imaging ◽  
Fluorescence Lifetime ◽  
Fluorescence Intensity ◽  
Time Domain ◽  
Ex Vivo ◽  
Renal Ischemia ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Lifetime Analysis

Fluorescence lifetime is an intrinsic parameter of the fluorescent probe, independent of the probe concentration but sensitive to changes in the surrounding microenvironment. Therefore, fluorescence lifetime imaging could potentially be applied to in vivo diagnostic assessment of changes in the tissue microenvironment caused by disease, such as ischemia. The aim of this study was to evaluate the utility of noninvasive fluorescence lifetime imaging in distinguishing between normal and ischemic kidney tissue in vivo. Mice were subjected to 60-minute unilateral kidney ischemia followed by 6-hour reperfusion. Animals were then injected with the near-infrared fluorescence probe Cy5.5 or saline and imaged using a time-domain small-animal optical imaging system. Both fluorescence intensity and lifetime were acquired. The fluorescence intensity of Cy5.5 was clearly reduced in the ischemic compared with the contralateral kidney, and the fluorescence lifetime of Cy5.5 was not detected in the ischemic kidney, suggesting reduced kidney clearance. Interestingly, the two-component lifetime analysis of endogenous fluorescence at 700 nm distinguished renal ischemia in vivo without the need for Cy5.5 injection for contrast enhancement. The average fluorescence lifetime of endogenous tissue fluorophores was a sensitive indicator of kidney ischemia ex vivo. The study suggests that fluorescence lifetime analysis of endogenous tissue fluorophores could be used to discriminate ischemic or necrotic tissues by noninvasive in vivo or ex vivo organ imaging.

On-the-Fly Fluorescence Lifetime Detection of Humic Substances in Capillary Electrophoresis

2003 ◽  
Vol 57 (3) ◽  
pp. 256-265 ◽  
Author(s):  
Joseph D. Hewitt ◽  
Linda B. McGown
Keyword(s):  
Humic Substances ◽  
Fluorescence Lifetime ◽  
Spectral Characteristics ◽  
Emission Spectra ◽  
Fulvic Acids ◽  
Fluorescence Decay ◽  
Fluorescence Excitation ◽  
Lifetime Analysis ◽  
Decay Profile ◽  
Fluorescence Lifetime Detection

On-the-fly fluorescence lifetime detection was investigated as a tool for studying humic substances in capillary zone electrophoresis (CZE). Humic substances are complex, heterogeneous mixtures of natural products that tend to migrate in a single, broad CZE peak. The intrinsic fluorescence lifetime of five humic substances from the International Humic Substances Society (IHSS) was monitored using excitation at 488 or 364 nm to produce intensity-lifetime electropherograms for each of the substances. Each frequency-domain lifetime measurement, collected at sub-second intervals during the CZE run, contains the equivalent of a complete decay profile. Lifetime analysis of each decay profile was used to construct a lifetime-resolved electropherogram for each lifetime component, from which the variation in relative intensity contributions of each lifetime across the broad CZE peak could be determined. Absorption spectra, fluorescence excitation–emission spectra, and lifetime profiles of batch solutions of the samples were determined as well. It was found that, whereas absorption and fluorescence spectral characteristics tended to discriminate between humic acids and fulvic acids, the batch solution lifetime profiles discriminated instead between samples from different sources, regardless of fraction. On-the-fly lifetime detection provided a more detailed view of the fluorescence decay of the samples, including greater resolution of lifetimes for two of the fulvic acids and greater discrimination among samples based on lifetime profiles across the CZE peaks.

Fluorescence Lifetime Analysis of Nitrite Reductase fromAlcaligenes xylosoxidansat the Single-Molecule Level Reveals the Enzyme Mechanism

2011 ◽  
Vol 17 (43) ◽  
pp. 12015-12019 ◽  
Author(s):  
Leandro C. Tabares ◽  
Dorota Kostrz ◽  
Abdalmohsen Elmalk ◽  
Alessio Andreoni ◽  
Christopher Dennison ◽  
...  
Keyword(s):  
Single Molecule ◽  
Fluorescence Lifetime ◽  
Nitrite Reductase ◽  
Enzyme Mechanism ◽  
Single Molecule Level ◽  
Lifetime Analysis

scholarly journals Three-Color Single-Molecule FRET and Fluorescence Lifetime Analysis of Fast Protein Folding

2019 ◽  
Vol 116 (3) ◽  
pp. 138a-139a
Author(s):  
Janghyun Yoo ◽  
John M. Louis ◽  
Irina V. Gopich ◽  
Hoi Sung Chung
Keyword(s):  
Protein Folding ◽  
Single Molecule ◽  
Fluorescence Lifetime ◽  
Single Molecule Fret ◽  
Lifetime Analysis

Fluorescence Lifetime Analysis of Graphene Quantum Dots

2014 ◽  
Vol 118 (51) ◽  
pp. 30282-30290 ◽  
Author(s):  
Magnus Röding ◽  
Siobhan J. Bradley ◽  
Magnus Nydén ◽  
Thomas Nann
Keyword(s):  
Quantum Dots ◽  
Fluorescence Lifetime ◽  
Graphene Quantum Dots ◽  
Lifetime Analysis
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