Holography cytology for imaging cellular biomechanics

2021 ◽  
Author(s):  
Adam P. Wax
Keyword(s):  
Cellular Biomechanics

scholarly journals Plexin-B2 facilitates glioblastoma infiltration by modulating cell biomechanics

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Yong Huang ◽  
Rut Tejero ◽  
Vivian K. Lee ◽  
Concetta Brusco ◽  
Theodore Hannah ◽  
...  
Keyword(s):  
Cellular Level ◽  
Brain Parenchyma ◽  
Matrix Stiffness ◽  
Tumor Spread ◽  
Cell Biomechanics ◽  
Fiber Tracts ◽  
Downstream Effectors ◽  
Cellular Biomechanics ◽  
Matrix Interactions ◽  
Stem Cell Lines

AbstractInfiltrative growth is a major cause of high lethality of malignant brain tumors such as glioblastoma (GBM). We show here that GBM cells upregulate guidance receptor Plexin-B2 to gain invasiveness. Deletion of Plexin-B2 in GBM stem cells limited tumor spread and shifted invasion paths from axon fiber tracts to perivascular routes. On a cellular level, Plexin-B2 adjusts cell adhesiveness, migratory responses to different matrix stiffness, and actomyosin dynamics, thus empowering GBM cells to leave stiff tumor bulk and infiltrate softer brain parenchyma. Correspondingly, gene signatures affected by Plexin-B2 were associated with locomotor regulation, matrix interactions, and cellular biomechanics. On a molecular level, the intracellular Ras-GAP domain contributed to Plexin-B2 function, while the signaling relationship with downstream effectors Rap1/2 appeared variable between GBM stem cell lines, reflecting intertumoral heterogeneity. Our studies establish Plexin-B2 as a modulator of cell biomechanics that is usurped by GBM cells to gain invasiveness.

APPLICATION OF BIOMECHANICS TO TISSUE ENGINEERING: A PERSONAL VIEW

2008 ◽  
Vol 08 (02) ◽  
pp. 153-160 ◽  
Author(s):  
BRUCE K. MILTHORPE
Keyword(s):  
Tissue Engineering ◽  
Mechanical Stimuli ◽  
Personal View ◽  
Tissue Engineering Scaffolds ◽  
Cellular Behavior ◽  
Cellular Biomechanics ◽  
Biological Studies ◽  
Biomechanical Stimuli ◽  
Relationship Of

Cellular biomechanics is an area of study that is receiving more attention as time progresses. The response of cells to their mechanical environment, including biomechanical stimuli, has far-reaching ramifications for the area of tissue engineering, especially for tissues designed to withstand mechanical loading (e.g. bone, cartilage, tendons and ligaments, and arteries). The effects of mechanical stimuli on cells are only recently being examined, and the potential role of mechanical stimuli in tissue engineering is still one that is largely ignored in the design of tissue engineering scaffolds. The relationship of mechanical properties of scaffolds or of mechanical stimuli to cell behavior is complex, but vital to the development of the field. Also, understanding the complex interplay of form and environment on cells involves an increase in our knowledge of how cells react to their total environment including mechanical stimuli and material properties. In order to improve tissue engineering outcomes, a nexus must be developed between the mechanical, biochemical, and biological studies of cellular behavior, in the context of extremely complex systems.

scholarly journals Apoptotic human neutrophil peptide-1 anti-tumor activity revealed by cellular biomechanics

2015 ◽  
Vol 1853 (2) ◽  
pp. 308-316 ◽  
Author(s):  
Diana Gaspar ◽  
João M. Freire ◽  
Teresa R. Pacheco ◽  
João T. Barata ◽  
Miguel A.R.B. Castanho
Keyword(s):  
Human Neutrophil ◽  
Cellular Biomechanics ◽  
Anti Tumor Activity ◽  
Human Neutrophil Peptide

scholarly journals MACC1 driven alterations in cellular biomechanics facilitate cell motility in glioblastoma

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Tim Hohmann ◽  
Urszula Hohmann ◽  
Marc R. Kolbe ◽  
Mathias Dahlmann ◽  
Dennis Kobelt ◽  
...  
Keyword(s):  
Cell Motility ◽  
Cellular Biomechanics

scholarly journals Cellular Biomechanics in Drug Screening and Evaluation: Mechanopharmacology

2016 ◽  
Vol 37 (2) ◽  
pp. 87-100 ◽  
Author(s):  
Ramaswamy Krishnan ◽  
Jin-Ah Park ◽  
Chun Y. Seow ◽  
Peter V-S. Lee ◽  
Alastair G. Stewart
Keyword(s):  
Drug Screening ◽  
Cellular Biomechanics
Sign in / Sign up

Export Citation Format

Share Document